Amplified Photodegradation of Cell‐Laden Hydrogels via an Addition–Fragmentation Chain Transfer Reaction

Brown, Tobin E.; Marozas, Ian A.; Anseth, Kristi S.

doi:10.1002/adma.201605001

Cited by 92 publications

(84 citation statements)

References 58 publications

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“…[21] Recent advances have demonstrated the delivery of proteins cued by longer wavelengths (600 – 900 nm) using a red-shifted azobenzene guest-β-cyclodextran host interaction or upconverting nanoparticles, [22,23] and the development of a hydrogel that responds rapidly to near-UV light. [24] However, there remain significant challenges to combine longer-wavelength-absorbing phototriggers with rapid hydrogel responses, as well as improving chemical syntheses in such materials systems.…”

mentioning

confidence: 99%

Ruthenium‐Crosslinked Hydrogels with Rapid, Visible‐Light Degradation

Rapp

Highley

Manor

et al. 2018

Chemistry A European J

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Incorporation of photoresponsive molecules within soft materials can provide spatiotemporal control over bulk properties and address challenges in targeted delivery and mechanical variability. However, the kinetics of in situ photochemical reactions are often slow and typically employ ultraviolet wavelengths. Here, we present a novel photoactive crosslinker Ru(bipyridine) (3-pyridinaldehyde) (RuAldehyde), which was reacted with hydrazide-functionalized hyaluronic acid to form hydrogels capable of encapsulating protein cargo. Visible light irradiation (400-500 nm) initiated rapid ligand exchange on the ruthenium center, which degraded the hydrogel within seconds to minutes, depending on gel thickness. An exemplar enzyme cargo, TEM1 β-lactamase, was loaded into and photoreleased from the Ru-hydrogel. To expand their applications, Ru-hydrogels were also processed into microgels using a microfluidic platform.

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mentioning

confidence: 99%

Ruthenium‐Crosslinked Hydrogels with Rapid, Visible‐Light Degradation

Rapp

Highley

Manor

et al. 2018

Chemistry A European J

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“…Depending on which cell-material interactions one wishes to control or the end application of the resulting biomaterial, photodegradation has been used to create spatiotemporal differences in material stiffness or biochemical composition, 189 varied topolographical features, 190,191 or complete dissolution of a preformed gel. 192 …”

Section: Photocleavage Reactionsmentioning

confidence: 99%

“…222–224 Of these, the strain-promoted azide alkyne cycloaddition (SPAAC) has, so far, proven most useful. 88,96,150,192,207,212,213,220 …”

Section: Photocleavage Reactionsmentioning

confidence: 99%

“…In these reports, functional groups that are susceptible to radical cleavage, such as disulfides 225 and allyl sulfides, 192 are incorporated into the hydrogel backbone (Figure 11a). When a soluble photoinitiator is introduced and upon exposure to light, photogenerated radicals cause photocleavage reactions.…”

Section: Photocleavage Reactionsmentioning

confidence: 99%

“…For example, Brown et al demonstrated the erosion of a 1 cm thick hydrogel sample in 1 minute of irradiation with 10 mW cm −2 of 365 nm light (Figure 11c). 192 The radical-based photodegradation mechanism can also be used to allow network reorganization during irradiation, for example, the creation of topographical features by patterned stress relaxation during impression with a textured surface (Figure 11b). 225 This photodegradation strategy is likely to find use in cases where thicker samples are used and/or when longer irradiation times are not practical.…”

Section: Photocleavage Reactionsmentioning

confidence: 99%

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Spatiotemporal hydrogel biomaterials for regenerative medicine

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4D Biomaterials for Light‐Guided Angiogenesis

Farrukh

Paez

Campo

2018

Adv Funct Materials

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A strategy for spatial and temporal regulation of ligand presentation within a biomaterial, and the consequent site‐ and time‐specific cellular responses in 4D cell cultures are presented. The key molecular component is a light‐activatable adhesive peptidomimetic (cyclo Arg‐Gly‐Asp‐phe‐Cys, RGDfC) modified with the two‐photon photocleavable group (p‐methoxynitrobiphenyl, PMNB) used to functionalize a hydrogel. A scanning laser at 740 nm defines the 4D presentation of active RGD ligands within the gel, and directs basic cellular processes of embedded cells in situ. The excellent photochemical properties of the PMNB photoremovable group allows direct photomanipulation of the cellular environment without appreciable damage of the embedded cells. Light‐directed migration of fibroblasts within a crosslinked poly(ethylene glycol) (PEG) hydrogel, and sequential, light‐regulated angiogenesis with human umbilical vein endothelial cells (HUVECs) in 4D constructs is demonstrated. The materials presented here represent unique microenvironments to reconstruct dynamic changes in the composition of the extracellular space of cells that occur in in vivo tissues.

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Amplified Photodegradation of Cell‐Laden Hydrogels via an Addition–Fragmentation Chain Transfer Reaction

Cited by 92 publications

References 58 publications

Ruthenium‐Crosslinked Hydrogels with Rapid, Visible‐Light Degradation

Ruthenium‐Crosslinked Hydrogels with Rapid, Visible‐Light Degradation

Spatiotemporal hydrogel biomaterials for regenerative medicine

4D Biomaterials for Light‐Guided Angiogenesis

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