Amplified fragment length polymorphism analysis of grapevine rootstock genotypes in Turkey

Abstract

ABSTRACT. Amplified fragment length polymorphism (AFLP) technique was used to assess genetic relationships among 20 grapevine rootstocks in Turkey. Discrimination of the rootstocks with 10 primer combinations yielded 1366 bands on AFLP gels; 65% of them were polymorphic. The rootstocks revealed two main clusters; one of them comprised two (Malégue and Harmony) and the other 18 genotypes. The Ber x Rip hybrids Cosmo 2 and Cosmo 10 formed a group with a high internal similarity ratio (0.909); they also formed a group with other Ber x Rip hybrids, 5C, 8B, SO4, and 420A Mgt, with a similarity ratio higher than 0.690 (subcluster II). Rootstock 5BB was placed in another subcluster (subcluster III). Among five Ber x Rup rootstocks, 110R-99R (0.853) and 1103P-140Ru (0.837), which were located in different subclusters, formed a dual group, as expected. Rootstock 779P, which had almost 0.800 similarity with the dual group of 110R-99R, formed another group. The 44-53 Malégue and Harmony rootstocks formed a group with the lowest similarity ratio (0.668) (subcluster I) and 41B-Fercal formed another dual group with a high similarity ratio (0.813). The distinction capacity of single-and double-EcoRI-MseI primers was evaluated; primers AC/CTA, TC/CAC, AG/CTC, and AG/CAG discriminated the 20 rootstocks, with a similarity value below 0.910. The best primers for discrimination of rootstock varieties were AG/ CAG and AG/CTC, while the primers TC/CAC and AC/CTA could also be useful for clonal discrimination of genotypes.

Molecular characterization of papaya genotypes using AFLP markers

“…Among the tested primers, E-ACT x M-CTG, E-ACT x M-CTT, E-AAC x M-CAA, E-AAC x M-CAC, E-AAC x M-CAG and E-AAC x M-CAT were important combinations for either germplasm screening, landrace accessions, inbred lines and other studies like genetic mapping and molecular assisted selection. These results are in agreement with others crop such as grapevine (ERGÜL et al, 2010), where 4 single and 20 double AFLP-primer combinations were shown to be successful discriminate 20 rootstocks; and soybean, where it was possible to discriminate each one of the 44 soybean genotypes using five AFLP-primer combinations (SINGH et al, 2010).…”

High genetic differentiation of grapevine rootstock varieties determined by molecular markers and artificial neural networks

“…Molecular characterization in rootstocks is mainly employed to compare other V. vinifera cultivars from collections in the largest germplasm banks of Vitis. Molecular markers, such as restriction fragment length polymorphism (RFLP) (Bourquin, Otten, & Walter, 1991;Bourquin, Tournier, Otten, & Walter, 1992;Guerra & Meredith, 1995), inter simple sequence repeat (ISSR) (Alizadeh, Singh, Jhang, & Sharma, 2008), amplified fragments length polymorphism (AFLP) (Ergül, Aras, & Söylemezoğlu, 2010;Sabir, Doğan, Tangolar, & Kafkas, 2010), simple repeated sequences (SSR) (Sefc, Regner, Glössl, & Steinkellner, 1998;Dzhambazova et al, 2007;Laucou et al, 2011;Jahnke, Majer, Varga, & Szöke, 2011;Andre et al, 2012;Sant'Ana et al, 2012;Emanuelli et al, 2013;Barankova, 2014), and some methods based on retrotransposons (Guo, Guo, Hou, & Zhang, 2014), have been used to identify polymorphic loci for the measurement of genetic divergence or similarity between rootstock varieties.…”