Amplified androstenedione enzymeimmunoassay for the diagnosis of cryptorchidism in the male horse: comparison with testosterone and estrone sulphate methods

Illera, J.C.; Silván, Gema; Munro, Coralie; Lorenzo, P.L.; Illera, M.; Liu, I.K.M.

doi:10.1016/s0960-0760(03)00057-8

Cited by 20 publications

(26 citation statements)

References 22 publications

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“…Illera et al . () evaluated the use of serum androstenedione but inconsistency in their results significantly weakens the evidence provided by this paper. The most recent experimental study by Claes et al .…”

Section: Quantity and Quality Of Evidencementioning

confidence: 80%

“…), serum androstenedione (Illera et al . ) and faecal oestrogens (Palme et al . ) to distinguish between gelded, entire and cryptorchid horses.…”

Section: Quantity and Quality Of Evidencementioning

confidence: 99%

“…; Illera et al . ) and accuracy may be improved by testosterone measurement up to 72 h post hCG (Silberzahn et al . ).…”

Section: Clinical Relevancementioning

confidence: 99%

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What is the best test for cryptorchidism?

Parker¹

2015

Equine Veterinary Education

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Section: Quantity and Quality Of Evidencementioning

confidence: 80%

“…), serum androstenedione (Illera et al . ) and faecal oestrogens (Palme et al . ) to distinguish between gelded, entire and cryptorchid horses.…”

Section: Quantity and Quality Of Evidencementioning

confidence: 99%

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What is the best test for cryptorchidism?

Parker¹

2015

Equine Veterinary Education

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“…Markedly reduced expression of 3 -HSD has been also reported in rats with experimentally induced cryptorchidism (Wisner & Gomes, 1978). No significant changes in T levels have been detected in patients with cryptorchidism as well as other mammalian species Farrer et al, 1985;Illera et al, 2003;Kawakami et al, 1999;Ren et al, 2006;Ryan et al, 1986). …”

Section: Temperature and 3β-hsd Activitymentioning

confidence: 93%

Hydroxysteroid Dehydrogenases – Localization, Function and Regulation in the Testis

Kotula–Balak¹,

Hejmej²,

Bilińska³

2012

Dehydrogenases

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“…The DHEA, androstenedione, testosterone and 17β-oestradiol concentrations were measured on serum samples after storing at -70 ºC for less than two weeks. Hormones were assayed by amplified enzyme immunoassay EIA as described by Illera et al (2003) and were expressed in nmol/l, except for 17β-oestradiol (in pmol/l).…”

Section: Methodsmentioning

confidence: 99%

Changes in serum dehydroepiandrosterone, androstenedione, testosterone, and 17β-oestradiol levels associated with disease and surgery in the horse

Ayala

Martos

2013

Acta Vet. Brno

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The aim of this work was to measure serum concentrations of dehydroepiandrosterone, androstenedione, testosterone and 17β-oestradiol in horses with various diseases and after surgery. We hypothesize that diseases and castration could potentially affect concentrations of steroid reproductive hormones. Blood samples were obtained from six groups of horses comprising a total of 119 horses (75 males and 44 females, 5-15 years old) with laminitis, acute abdominal syndrome, acute diseases, chronic diseases, after castration and healthy control. Hormone concentrations in serum were determined for each group using competitive enzyme immunoassay. Significant increases compared to control were found for dehydroepiandrosterone in horses with castration (P < 0.01), acute abdominal syndrome and acute diseases (P < 0.05). Besides, significant increases were observed for androstenedione in horses with laminitis, castration and acute diseases (P < 0.01), and in acute abdominal syndrome and chronic diseases (P < 0.05). Significant increases were also found for testosterone in horses with castration (P < 0.01) and with laminitis, acute abdominal syndrome and chronic diseases (P < 0.05). The lowest values of testosterone were found in the control group. Compared to control, 17b-oestradiol serum concentrations showed significant decreases (P < 0.01) in horses with laminitis, acute abdominal syndrome, acute and chronic diseases. Significant differences (P < 0.05) for the four studied hormones were found between males and females in each group. Our results showed that there were significant differences in steroid reproductive hormone concentrations in diseased horses and in those after surgery, compared to controls. Equine, steroid reproductive hormone levels, serum, EIA No detailed comparative data are available on steroid reproductive hormone concentrations in horses with various diseases and after surgery. We hypothesize that these pathological situations or surgery could potentially affect concentrations of steroid reproductive hormones. In fact, the response of the hypothalamic-pituitary-adrenal (HPA) axis and adrenal medulla has been assessed in horses with various diseases and following surgery (Ayala et al. 2011). Several HPA axis components are modulated by gonadal steroids and pregnolone is the precursor to cortisol and cortisone (Figueiredo et al. 2002). The aim of the present study was to measure serum concentrations of steroid reproductive hormones in horses with various diseases (acute and chronic) or after surgery, whilst avoiding the potential influence of other factors (time of sampling, transport, therapy and season), and to provide data for assessing the relationship between disease and reproductive endocrine system activity. Materials and Methods A total of 95 Spanish-bred horses diseased or referred for castration (Table 1) admitted to the Veterinary Clinical Hospital of the University of Murcia in Spain were studied, in addition to 24 horses in the control group. Only clinical cases admitted during morning hours...

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Amplified androstenedione enzymeimmunoassay for the diagnosis of cryptorchidism in the male horse: comparison with testosterone and estrone sulphate methods

Cited by 20 publications

References 22 publications

What is the best test for cryptorchidism?

What is the best test for cryptorchidism?

Hydroxysteroid Dehydrogenases – Localization, Function and Regulation in the Testis

Changes in serum dehydroepiandrosterone, androstenedione, testosterone, and 17β-oestradiol levels associated with disease and surgery in the horse

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