Hydroxysteroid Dehydrogenases – Localization, Function and Regulation in the Testis

Kotula–Balak, Małgorzata; Hejmej, Anna; Bilińska, Barbara

doi:10.5772/47740

Cited by 4 publications

(1 citation statement)

References 141 publications

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“…Next, to confirm that the AAV9-inducible Cre/ LoxP system specifically targets Leydig cells within the testis, we co-localised the Leydig cell marker 3βHSD and RFP [ 32 ]. RFP localisation was restricted to 3βHSD-positive Leydig cells in the testis ( Figure 3 C).…”

Section: Resultsmentioning

confidence: 99%

A Novel Model Using AAV9-Cre to Knockout Adult Leydig Cell Gene Expression Reveals a Physiological Role of Glucocorticoid Receptor Signalling in Leydig Cell Function

Gannon

Darbey

Chensee

et al. 2022

IJMS

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Glucocorticoids are steroids involved in key physiological processes such as development, metabolism, inflammatory and stress responses and are mostly used exogenously as medications to treat various inflammation-based conditions. They act via the glucocorticoid receptor (GR) expressed in most cells. Exogenous glucocorticoids can negatively impact the function of the Leydig cells in the testis, leading to decreased androgen production. However, endogenous glucocorticoids are produced by the adrenal and within the testis, but whether their action on GR in Leydig cells regulates steroidogenesis is unknown. This study aimed to define the role of endogenous GR signalling in adult Leydig cells. We developed and compared two models; an inducible Cre transgene driven by expression of the Cyp17a1 steroidogenic gene (Cyp17-iCre) that depletes GR during development and a viral vector-driven Cre (AAV9-Cre) to deplete GR in adulthood. The delivery of AAV9-Cre ablated GR in adult mouse Leydig cells depleted Leydig cell GR more efficiently than the Cyp17-iCre model. Importantly, adult depletion of GR in Leydig cells caused reduced expression of luteinising hormone receptor (Lhcgr) and of steroidogenic enzymes required for normal androgen production. These findings reveal that Leydig cell GR signalling plays a physiological role in the testis and highlight that a normal balance of glucocorticoid activity in the testis is important for steroidogenesis.

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Section: Resultsmentioning

confidence: 99%

A Novel Model Using AAV9-Cre to Knockout Adult Leydig Cell Gene Expression Reveals a Physiological Role of Glucocorticoid Receptor Signalling in Leydig Cell Function

Gannon

Darbey

Chensee

et al. 2022

IJMS

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Interrelationship between NO and androgenic activity in mice, Mus musculus, following temporal phase relation of serotonergic and dopaminergic neural oscillations

Singh

Chaturvedi

2014

Endocrine

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The present study was designed to monitor the correlation of gonadal steroidogenic activity and nitric oxide (NO) of plasma and testis of male laboratory mouse, Mus musculus, under (1) control/basal and (2) experimental conditions. In the first study, male mice (n = 6) of three age groups (3-week-old sexually immature, 9-week-old sexually mature and 1.5-year-old) were assayed for plasma testosterone and level of NO in the plasma and testis. The immunoreactivity of 3-β-hydroxysteroid dehydrogenase (3-β-HSD) and androgen receptors (ARs) was also detected in testis and epididymis, respectively. In the second study, the reproductive state of mice was altered by injecting precursors of serotonin 5-hydroxytryptophan (5-HTP) and dopamine L-dihydroxyphenylalanine (L-DOPA) daily for 13 days. In one subgroup, mice received their daily injections 8 h apart and in another subgroup 12 h apart. A third subgroup of control mice received two daily injections of normal saline. After the completion of the experiment, the mentioned parameters were measured. The results showed a significant increase in levels of NO and a lowering of the immune reactivity of 3-β-HSD and AR, in both leydig cells and the epididymis in the sexually immature, old-age mice as well as in the mice that received 5-HTP and L-DOPA in the 8-h temporal relation, whereas opposite effects were observed in the sexually mature mice as well as in the mice that received 5-HTP and L-DOPA in the 12-h temporal relation. These findings led us to conclude that an inverse correlation exists between testicular steroidogenic activity and NO activity of laboratory mice under control and experimentally modulated gonadal conditions.

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Buchholzia coriacea seed induce antifertility by interfering with steroidogenic enzymes and inflammatory cytokines in rat testis

Obembe

Ojetola

Atere

et al. 2023

Journal of Reproductive Immunology

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Hydroxysteroid Dehydrogenases – Localization, Function and Regulation in the Testis

Cited by 4 publications

References 141 publications

A Novel Model Using AAV9-Cre to Knockout Adult Leydig Cell Gene Expression Reveals a Physiological Role of Glucocorticoid Receptor Signalling in Leydig Cell Function

A Novel Model Using AAV9-Cre to Knockout Adult Leydig Cell Gene Expression Reveals a Physiological Role of Glucocorticoid Receptor Signalling in Leydig Cell Function

Interrelationship between NO and androgenic activity in mice, Mus musculus, following temporal phase relation of serotonergic and dopaminergic neural oscillations

Buchholzia coriacea seed induce antifertility by interfering with steroidogenic enzymes and inflammatory cytokines in rat testis

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