Amplification and up-regulation of microRNA-30b in oral squamous cell cancers

Shao, Chenying; Yu, Yili; Yu, Li-Ying; Yuan, Pengbo; Feng, QiPing; Chu, Fei; Fang, Zhengyu; Zhou, Yi

doi:10.1016/j.archoralbio.2012.04.002

Cited by 21 publications

(18 citation statements)

References 27 publications

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“…However, studies of cervical and esophageal cancer noticed that miR-130a upregulation was associated with tumor development and poor prognosis (49,53), in line with our results in tumor vs. healthy cells. Similarly, in a study of 107 samples of oral cavity SCC, the copy number of mir-30b was frequently increased in advanced tumors, in correlation with hsa-miR-30b overexpression (54). …”

Section: Discussionmentioning

confidence: 94%

Distinctive pattern of let-7 family microRNAs in aggressive carcinoma of the oral tongue in young patients

et al. 2016

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Oral cavity squamous cell carcinoma may be more aggressive at presentation and recurrence in young patients compared with older patients. Dysregulation of microRNAs (miRNAs or miRs) has been associated with the development and prognosis of oral cavity cancer. The present study investigated miRNA expression in carcinoma of the oral tongue in young patients. miRNA expression profiles were evaluated in formalin-fixed, paraffin-embedded samples of tumor and normal mucosa from 12 patients aged <30 years old with squamous cell carcinoma of the tongue. The levels of let-7f-5p, miR-30b-5p and let-7e-5p were upregulated in tumors (P<0.05). The expression of let-7f-5p was upregulated in non-aggressive tumors, while the expression of let-7e-5p was upregulated in aggressive tumors, compared with the corresponding normal tissue. Aggressive tumors had higher levels of let-7c, miR-130a-3p, miR-361-5p, miR-99a-5p, miR-29c-3p and let-7d-5p than non-aggressive tumors (P<0.05). The findings remained significant for let-7c upon false-discovery rate correction. An excellent correlation was noticed on validation of NanoString counts by quantitative polymerase chain reaction. The comparison with published findings in adults demonstrated a unique miRNA signature in young patients with aggressive disease. Aggressive oral cavity cancer in patients <30 years old is associated with a distinctive expression pattern of the let-7 family. Larger studies including direct comparison with older patients are warranted.

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Section: Discussionmentioning

confidence: 94%

Distinctive pattern of let-7 family microRNAs in aggressive carcinoma of the oral tongue in young patients

et al. 2016

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“…Recent data, however, link serum miR-19a with bad prognosis in NSCLC [45]. Similarly, miR-30b is also suggested as a possible oncomiR as it was shown to be overexpressed in oral SCC [46] and in the serum of rats bearing pancreatic ductal adenocarcinoma [47]. …”

Section: Discussionmentioning

confidence: 99%

Comparisons of microRNA Patterns in Plasma before and after Tumor Removal Reveal New Biomarkers of Lung Squamous Cell Carcinoma

Zborovskaya²,

et al. 2013

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Lung cancer is the major human malignancy, accounting for 30% of all cancer-related deaths worldwide. Poor survival of lung cancer patients, together with late diagnosis and resistance to classic chemotherapy, highlights the need for identification of new biomarkers for early detection. Among different cancer biomarkers, small non-coding RNAs called microRNAs (miRNAs) are considered the most promising, owing to their remarkable stability, their cancer-type specificity, and their presence in body fluids. However, results of multiple previous attempts to identify circulating miRNAs specific for lung cancer are inconsistent, likely due to two main reasons: prominent variability in blood miRNA content among individuals and difficulties in distinguishing tumor-relevant miRNAs in the blood from their non-tumor counterparts. To overcome these impediments, we compared circulating miRNA profiles in patients with lung squamous cell carcinoma (SCC) before and after tumor removal, assuming that the levels of all tumor-relevant miRNAs would drop after the surgery. Our results revealed a specific panel of the miRNAs (miR-205, -19a, -19b, -30b, and -20a) whose levels decreased strikingly in the blood of patients after lung SCC surgery. Interestingly, miRNA profiling of plasma fractions of lung SCC patients revealed high levels of these miRNA species in tumor-specific exosomes; additionally, some of these miRNAs were also found to be selectively secreted to the medium by cultivated lung cancer cells. These results strengthen the notion that tumor cells secrete miRNA-containing exosomes into circulation, and that miRNA profiling of the exosomal plasma fraction may reveal powerful cancer biomarkers.

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“…Since little experimental information is available on potential target genes and miRNA cluster identified in our study and we have not experimentally carried out analysis of gain- and loss-of function, we used experimental data on miRNA function to understand the potential implication and relevance of these miRNAs in the development and progression of (patho)-physiological conditions. For example, miRNA-30b has been implicated in angiogenesis [40], TRAIL-induced apoptosis in glioma cells [41] and oral squamous cell cancers [42]. miR-193a-5p targets YY1-APC regulatory axis in human endometrioid endometrial adenocarcinoma [43].…”

Section: Discussionmentioning

confidence: 99%

Identification of a set of miRNAs differentially expressed in transiently TIA-depleted HeLa cells by genome-wide profiling

et al. 2013

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BackgroundT-cell intracellular antigen (TIA) proteins function as regulators of cell homeostasis. These proteins control gene expression globally at multiple levels in response to dynamic regulatory changes and environmental stresses. Herein we identified a micro(mi)RNA signature associated to transiently TIA-depleted HeLa cells and analyzed the potential role of miRNAs combining genome-wide analysis data on mRNA and miRNA profiles.ResultsUsing high-throughput miRNA expression profiling, transient depletion of TIA-proteins in HeLa cells was observed to promote significant and reproducible changes affecting to a pool of up-regulated miRNAs involving miR-30b-3p, miR125a-3p, miR-193a-5p, miR-197-3p, miR-203a, miR-210, miR-371-5p, miR-373-5p, miR-483-5p, miR-492, miR-498, miR-503-5p, miR-572, miR-586, miR-612, miR-615-3p, miR-623, miR-625-5p, miR-629-5p, miR-638, miR-658, miR-663a, miR-671-5p, miR-769-3p and miR-744-5p. Some up-regulated and unchanged miRNAs were validated and previous results confirmed by reverse transcription and real time PCR. By target prediction of the miRNAs and combined analysis of the genome-wide expression profiles identified in TIA-depleted HeLa cells, we detected connections between up-regulated miRNAs and potential target genes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) database analysis suggest that target genes are related with biological processes associated to the regulation of DNA-dependent transcription, signal transduction and multicellular organismal development as well as with the enrichment of pathways involved in cancer, focal adhesion, regulation of actin cytoskeleton, endocytosis and MAPK and Wnt signaling pathways, respectively. When the collection of experimentally defined differentially expressed genes in TIA-depleted HeLa cells was intersected with potential target genes only 7 out of 68 (10%) up- and 71 out of 328 (22%) down-regulated genes were shared. GO and KEGG database analyses showed that the enrichment categories of biological processes and cellular pathways were related with innate immune response, signal transduction, response to interleukin-1, glomerular basement membrane development as well as neuroactive ligand-receptor interaction, endocytosis, lysosomes and apoptosis, respectively.ConclusionAll this considered, these observations suggest that individual miRNAs could act as potential mediators of the epigenetic switch linking transcriptomic dynamics and cell phenotypes mediated by TIA proteins.

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Amplification and up-regulation of microRNA-30b in oral squamous cell cancers

Cited by 21 publications

References 27 publications

Distinctive pattern of let-7 family microRNAs in aggressive carcinoma of the oral tongue in young patients

Distinctive pattern of let-7 family microRNAs in aggressive carcinoma of the oral tongue in young patients

Comparisons of microRNA Patterns in Plasma before and after Tumor Removal Reveal New Biomarkers of Lung Squamous Cell Carcinoma

Identification of a set of miRNAs differentially expressed in transiently TIA-depleted HeLa cells by genome-wide profiling

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