Identification of a set of miRNAs differentially expressed in transiently TIA-depleted HeLa cells by genome-wide profiling

Sánchez-Jiménez, Carmen; Carrascoso, Isabel; Barrero, Juan J.; Izquierdo, José M.

doi:10.1186/1471-2199-14-4

Cited by 30 publications

(31 citation statements)

References 89 publications

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“…Hypoxia and ischemia in the tendon might have induced miR-498 which downregulated collagen type 1 expression but, additional studies are required to validate this aspect. In addition, miRNA-498 was found to be upregulated to control the metastasis of several cancer types including liver cancer, adenocarcinoma and retinoblastoma [45]. Also, increased expression of miR-498 has been correlated with the ECM damage associated with psoriasis [46].…”

Section: Discussionmentioning

confidence: 99%

“…The tissue sections were analyzed for the expression of Collagen I and Collagen III and MMP-2 and MMP-9 expression by immuno-double staining following our previously established standard protocols [13, 45, 50]. HIER buffer (Heat Induced Epitope Retrieval) was used for antigen retrieval at 95°C for 20 mins and the sections blocked in PBS containing 0.25% Triton X-100 and 5% horse serum for 2 hr at room temperature.…”

Section: Methodsmentioning

confidence: 99%

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MicroRNAs Associated with Shoulder Tendon Matrisome Disorganization in Glenohumeral Arthritis

et al. 2016

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The extracellular matrix (ECM) provides core support which is essential for the cell and tissue architectural development. The role of ECM in many pathological conditions has been well established and ECM-related abnormalities leading to serious consequences have been identified. Though much has been explored in regards to the role of ECM in soft tissue associated pathologies, very little is known about its role in inflammatory disorders in tendon. In this study, we performed microRNA (miRNA) expression analysis in the long head of the human shoulder biceps tendon to identify key genes whose expression was altered during inflammation in patients with glenohumeral arthritis. We identified differential regulation of matrix metalloproteinases (MMPs) that could be critical in collagen type replacement during tendinopathy. The miRNA profiling showed consistent results between the groups and revealed significant changes in the expression of seven different miRNAs in the inflamed tendons. Interestingly, all of these seven miRNAs were previously reported to have either a direct or indirect role in regulating the ECM organization in other pathological disorders. In addition, these miRNAs were also found to alter the expression levels of MMPs, which are the key matrix degrading enzymes associated with ECM-related abnormalities and pathologies. To our knowledge, this is the first report which identifies specific miRNAs associated with inflammation and the matrix reorganization in the tendons. Furthermore, the findings also support the potential role of these miRNAs in altering the collagen type ratio in the tendons during inflammation which is accompanied with differential expression of MMPs.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

MicroRNAs Associated with Shoulder Tendon Matrisome Disorganization in Glenohumeral Arthritis

et al. 2016

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“…TIA proteins are known to target genes with relevant biological implications in apoptosis, inflammation, cell responses to stress, viral infections and oncogenesis [1], [2], [18], [20]–[24]. Further, these proteins seem to have an important role during embryogenesis.…”

Section: Introductionmentioning

confidence: 99%

T-cell Intracellular Antigen (TIA)-Proteins Deficiency in Murine Embryonic Fibroblasts Alters Cell Cycle Progression and Induces Autophagy

Sánchez-Jiménez

Izquierdo

2013

PLoS ONE

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Mice lacking either T-cell intracellular antigen 1 (TIA1) or TIA1 related/like protein (TIAR/TIAL1) show high rates of embryonic lethality, suggesting a relevant role for these proteins during embryonic development. However, intrinsic molecular and cellular consequences of either TIA1 or TIAR deficiency remain poorly defined. By using genome-wide expression profiling approach, we demonstrate that either TIA1 or TIAR inactivation broadly alter normal development-associated signalling pathways in murine embryonic fibroblasts (MEF). Indeed, these analyses highlighted alterations of cytokine-cytokine and ECM-receptor interactions and Wnt, MAPK, TGF-beta dependent signalling pathways. Consistent with these results, TIA1 and TIAR knockout (KO) MEF show reduced rates of cell proliferation, cell cycle progression delay and increased cell size. Furthermore, TIA-proteins deficiency also caused metabolic deficiencies, increased ROS levels and DNA damage, promoting a gentle rise of cell death. Concomitantly, high rates of autophagy were detected in both TIA1 and TIAR KO MEF with induction of the formation of autophagosomes, as evidenced by the up-regulation of the LC3B protein, and autolysosomes, measured by colocalization of LC3B and LAMP1, as a survival mechanism attempt. Taken together, these observations support that TIA proteins orchestrate a transcriptome programme to activate specific developmental decisions. This program is likely to contribute to mouse physiology starting at early stages of the embryonic development. TIA1/TIAR might function as cell sensors to maintain homeostasis and promote adaptation/survival responses to developmental stress.

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“…People with a high level of miR-586 have relatively low expression levels of these genes, which leads to the high risk of aGVHD. Some target genes of miR-586 including SFPQ, KCTD12, FAM129A, TNRC6B, SLC12A2, and VPS13A were predicted using a bioinformatic tool [21]. However, the genes related to aGVHD need further elucidation.…”

Section: Discussionmentioning

confidence: 99%

Plasma microRNA-586 is a new biomarker for acute graft-versus-host disease

Wang

Zhao

et al. 2015

Ann Hematol

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Acute graft-versus-host disease (aGVHD) is one of the major causes of morbidity and mortality in patients receiving allogeneic hematopoietic cell transplantation (allo-HSCT). MicroRNAs (miRs) were found to have the potential to be the new biomarkers of aGVHD. In this study, we collected samples from 98 patients who underwent allo-HSCT; 63 patients developed aGVHD, and 35 patients did not. Plasma samples were collected at three time points (before aGVHD, at the onset of aGVHD, and after aGVHD) from 52 patients, and the miR-586 expression level was detected by quantitative real-time PCR. We found that the plasma miR-586 level was decreased at the onset of grade I-II aGVHD (P = 0.074). In contrast, when infections were detected, plasma miR-586 level was increased. Moreover, we detected the miR-586 expression level in patients who had infections but did not have aGVHD, and we found that miR-586 was upregulated (P = 0.005). We also compared the plasma miR-586 level at day 7 after transplantation between aGVHD patients and control patients. In the aGVHD group, there was a considerably higher miR-586 expression in comparison with the non-aGVHD group (P < 0.05). A more significant difference between the two groups was found when the patients with infections were excluded (P = 0.004). Furthermore, receive operating characteristic (ROC) analysis indicated that a higher expression level of miR-586 at day 7 could predict impending aGVHD. The optimal cutoff value of miR-586 to predict aGVHD was 2200 copies/μL with a sensitivity of 87.5 % and specificity of 55.0 %, and the area under the curve (AUC) was 0.739 (95 % CI 0.598-0.880, P = 0.004). Our study suggests that miR-586 might participate in the occurrence of aGVHD and could be a putative target for novel aGVHD therapy. The plasma level of miR-586 at day 7 after allo-HSCT would be a potential biomarker for predicting the occurrence of aGVHD.

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Identification of a set of miRNAs differentially expressed in transiently TIA-depleted HeLa cells by genome-wide profiling

Cited by 30 publications

References 89 publications

MicroRNAs Associated with Shoulder Tendon Matrisome Disorganization in Glenohumeral Arthritis

MicroRNAs Associated with Shoulder Tendon Matrisome Disorganization in Glenohumeral Arthritis

T-cell Intracellular Antigen (TIA)-Proteins Deficiency in Murine Embryonic Fibroblasts Alters Cell Cycle Progression and Induces Autophagy

Plasma microRNA-586 is a new biomarker for acute graft-versus-host disease

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