Abstract:The present study reported a single step synthesis of silver nanoparticles using ampicillin (Amp-AgNps), a second-generation β lactam antibiotic to get nanoformulation having dual properties that of antibiotic and silver. The Amp-AgNps was characterized by UV-VIS spectroscopy, TEM, XRD, FTIR and TGA. FTIR and TGA results suggested that amine group of Ampicllin reduce the metalic silver into nano form. These results were further validated by computational molecular dynamics simulation. The antibacterial potenti… Show more
“…Moreover, the effect of Fe 3 O 4 NPs also depends on the type of stabilizer. Stabilization of NPs by various compounds enhances an antimicrobial activity of NPs 8 , 26 , 31 . Figure 1 Specific growth rates of E. coli K-12 ( A ) kanamycin-resistant E. coli pARG-25 ( B ), and S. typhimurium MDC1759 ( C ) strains in the presence of citric acid coated Fe 3 O 4 and Ag NPs various concentrations.…”
The current research reports the antibacterial effects of silver (Ag) and citric acid coated iron oxide (Fe
3
O
4
) NPs on
Escherichia coli
wild type and kanamycin-resistant strains, as well as on
Salmonella typhimurium
MDC1759. NPs demonstrated significant antibacterial activity against these bacteria, but antibacterial effect of Ag NPs is more pronounced at low concentrations. Ag NPs inhibited 60–90% of
S. typhimurium
and drug-resistant
E. coli.
The latter is more sensitive to Fe
3
O
4
NPs than wild type strain: the number of bacterial colonies is decreased ~ 4-fold. To explain possible mechanisms of NPs action, H
+
-fluxes through the bacterial membrane and the H
+
-translocating F
O
F
1
-ATPase activity of bacterial membrane vesicles were studied.
N,N′-
Dicyclohexylcarbodiimide (DCCD)-sensitive ATPase activity was increased up to ~ 1.5-fold in the presence of Fe
3
O
4
NPs. ATPase activity was not detected by Ag NPs even in the presence of DCCD, which confirms the bactericidal effect of these NPs. The H
+
-fluxes were changed by NPs and by addition of DCCD. H
2
yield was inhibited by NPs; the inhibition by Ag NPs is stronger than by Fe
3
O
4
NPs. NPs showed antibacterial effect in bacteria studied in concentration-dependent manner by changing in membrane permeability and membrane-bound enzyme activity. The F
O
F
1
-ATPase is suggested might be a target for NPs.
“…Moreover, the effect of Fe 3 O 4 NPs also depends on the type of stabilizer. Stabilization of NPs by various compounds enhances an antimicrobial activity of NPs 8 , 26 , 31 . Figure 1 Specific growth rates of E. coli K-12 ( A ) kanamycin-resistant E. coli pARG-25 ( B ), and S. typhimurium MDC1759 ( C ) strains in the presence of citric acid coated Fe 3 O 4 and Ag NPs various concentrations.…”
The current research reports the antibacterial effects of silver (Ag) and citric acid coated iron oxide (Fe
3
O
4
) NPs on
Escherichia coli
wild type and kanamycin-resistant strains, as well as on
Salmonella typhimurium
MDC1759. NPs demonstrated significant antibacterial activity against these bacteria, but antibacterial effect of Ag NPs is more pronounced at low concentrations. Ag NPs inhibited 60–90% of
S. typhimurium
and drug-resistant
E. coli.
The latter is more sensitive to Fe
3
O
4
NPs than wild type strain: the number of bacterial colonies is decreased ~ 4-fold. To explain possible mechanisms of NPs action, H
+
-fluxes through the bacterial membrane and the H
+
-translocating F
O
F
1
-ATPase activity of bacterial membrane vesicles were studied.
N,N′-
Dicyclohexylcarbodiimide (DCCD)-sensitive ATPase activity was increased up to ~ 1.5-fold in the presence of Fe
3
O
4
NPs. ATPase activity was not detected by Ag NPs even in the presence of DCCD, which confirms the bactericidal effect of these NPs. The H
+
-fluxes were changed by NPs and by addition of DCCD. H
2
yield was inhibited by NPs; the inhibition by Ag NPs is stronger than by Fe
3
O
4
NPs. NPs showed antibacterial effect in bacteria studied in concentration-dependent manner by changing in membrane permeability and membrane-bound enzyme activity. The F
O
F
1
-ATPase is suggested might be a target for NPs.
“…Thus, allowing sufficient drugs to accumulate in the cytoplasm resulting in remarkable improvement in chemotherapy efficiency. [76][77][78][79][80][81]…”
Section: Nanoparticle Drug Delivery Systemmentioning
Colorectal cancer is one of the most common cancer diseases with the increase of cases prevalence >5% every year. Multidrug resistance mechanisms and non-localized therapy become primary problems of chemotherapy drugs for curing colorectal cancer disease. Therefore, the enteric-coated nanoparticle system has been studied and proved to be able to resolve those problems with good performance for colorectal cancer. The highlight of our review aims to summarize and discuss the enteric-coated nanoparticle drug delivery system specific for colorectal cancer disease. The main and supporting literatures were collected from published research articles of journals indexed in Scopus and PubMed databases. In the oral route of administration, Eudragit pH-sensitive copolymer as a coating agent prevents the degradation of the nanoparticle system from the gastric fluid and releases drug to intestinal-colon track. Therefore, it provides a colon-specific targeting ability. Impressively, enteric-coated nanoparticles having a sustained release profile significantly increase the cytotoxic effect of chemotherapeutic drugs and achieve cell-specific target delivery. The enteric-coated nanoparticle drug delivery system represents an excellent modification to improve the effectiveness and performance of anticancer drugs for colorectal cancer disease in terms of the oral route of administration.
“…Briefly, acidified KMnO 4 solution (0.630 mM, 10 ml) was prepared in 0.5 M H 2 SO 4 . Simultaneously, ascorbic acid standard solutions of varying concentrations (1,4,8,12,16, and 20 μg ml −1 ) were also prepared. Then, 0.1 ml of KMnO 4 was added to 1 ml of ascorbic acid solutions and incubated at room temperature for 5 min.…”
Section: Estimation Of Ascorbic Acid Content In Ig Extractmentioning
confidence: 99%
“…In this regard, different metallic nanoparticles (NPs) including silver (Ag) [4][5][6], gold (Au) [7], zinc (Zn) [8], selenium (Se) [9,10], titanium [11], ceria [12] have been explored [13]. Se is an essential metalloid element that has been extensively used in medical applications owing to its antioxidative, antimicrobial and antitumor activities [14].…”
The exploitation of plant extracts for the synthesis of nano selenium having antibacterial and antioxidant activities is an exciting approach to counteract the prevalence of infections caused by antibiotic-resistant bacteria, which holds relevance for medical and food industries. In the present work, a green and facile method for the preparation of nano selenium (nSe) using the fruit extract of Indian gooseberry (Phyllanthus Emblica) has been reported. The optical and structural properties of the as-synthesized nSe were studied through various characterization techniques. Eventually, the antioxidant potential of nSe was investigated via 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl free radical scavenging assays. Parallely, the antibacterial activity of nSe against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa was evaluated. The antioxidant assays indicated that even low dosage of nSe showed excellent activity with EC 50 values of 0.21 μg ml −1 and 3.34 μg ml −1 , respectively. Moreover, nSe exhibited significant inhibition in bacterial growth at low minimum inhibitory concentration (MIC) values against Escherichia coli (16 μg ml −1 ), Staphylococcus aureus (32 μg ml −1 ) and Pseudomonas aeruginosa (48 μg ml −1 ) compared to MIC values for standard drug ampicillin. Importantly, nSe did not induce any cytotoxic effects on normal human keratinocytes (HaCaT) at the tested concentrations; representing their biocompatible nature. The data obtained demonstrated the versatility of phytogreen nSe as a potent antioxidant and antibacterial agent to effectively prevent as well as treat multidrug-resistant bacterial infections.
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