Amphotericin B-Induced Oxidative Damage and Killing of Candida albicans

Sokol-Anderson, Marcia L.; Brajtburg, J; Medoff, Gerald

doi:10.1093/infdis/154.1.76

Cited by 259 publications

(167 citation statements)

References 20 publications

Supporting

Mentioning

148

Contrasting

Unclassified

Order By: Relevance

“…Our results revealed that SOD activity was signifi cantly increased in C. dubliniensis and C. albicans strains that were resistant to amphotericin B and fl uconazole compared to sensitive strains. The increase in SOD activity in response to amphotericin B may be due to the production of oxidative damage in Candida through lipid peroxidation 15 . In our study, yeast cultures were exposed to increasing concentrations of amphotericin B, up to a maximum of 2 µg ml -1 .…”

Section: Discussionmentioning

confidence: 99%

“…The enzyme catalase converts hydrogen peroxide (H 2 O 2 ) to oxygen (O 2 ) and water (H 2 O) 16 . In Candida, catalase has been suggested to be involved in the resistance to amphotericin B and is involved in hypha formation 15,28 . Catalase activity has not been previously studied in fl uconazole-resistant C. albicans and C. dubliniensis strains.…”

Section: Discussionmentioning

confidence: 99%

“…Ergosterol, a sterol present in the fungal plasma membrane, is the target of amphotericin B. The binding of amphotericin B to ergosterol produces channels that leak potassium ions, resulting in the disruption of the proton gradient 14,15 . The antioxidant enzymes SOD and catalase may act synergistically to protect cells from oxidative damage 16 .…”

mentioning

confidence: 99%

See 2 more Smart Citations

Fluconazole and amphotericin-B resistance are associated with increased catalase and superoxide dismutase activity in Candida albicans and Candida dubliniensis

Linares

Giacomelli

Altenhofen

et al. 2013

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

Introduction:Candida dubliniensis, a new species of Candida that has been recovered from several sites in healthy people, has been associated with recurrent episodes of oral candidiasis in AIDS and HIV-positive patients. This species is closely related to C. albicans. The enzymatic activity of C. dubliniensis in response to oxidative stress is of interest for the development of drugs to combat C. dubliniensis. Methods: Fluconazole-and amphotericin B-resistant strains were generated as described by FeketeForgács et al. (2000). Superoxide dismutase (SOD) and catalase assays were performed as described by McCord and Fridovich (1969) and Aebi (1984), respectively. Results: We demonstrated that superoxide dismutase (SOD) and cata lase activities were signifi cantly higher (p<0.05) in the fl uconazole-and amphotericin B-resistant strains of C. dubliniensis and C. albicans than in the sensitive strains. The catalase and SOD activities were also signifi cantly (p<0.01) higher in the sensitive and resistant C. albicans strains than in the respective C. dubliniensis strains. Conclusions: These data suggest that C. albicans is better protected from oxidative stress than C. dubliniensis and that fl uconazole, like amphotericin B, can induce oxidative stress in Candida; oxidative stress induces an adaptive response that results in a coordinated increase in catalase and SOD activities.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Fluconazole and amphotericin-B resistance are associated with increased catalase and superoxide dismutase activity in Candida albicans and Candida dubliniensis

Linares

Giacomelli

Altenhofen

et al. 2013

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

show abstract

“…Because AmB stimulates Fc-mediated ingestion and because it is known to exert some of its biological and toxic effects by the generation of reactive oxygen metabolites (9), we have investigated activation of the respiratory burst as a possible mechanism for regulation of phagocytic function. Superoxide and hydrogen peroxide production are known to accompany phagocytosis (10).…”

Section: Introductionmentioning

confidence: 99%

Studies on the molecular mechanisms of human Fc receptor-mediated phagocytosis. Amplification of ingestion is dependent on the generation of reactive oxygen metabolites and is deficient in polymorphonuclear leukocytes from patients with chronic granulomatous disease.

Gresham

Mcgarr²,

Shackelford³

et al. 1988

J. Clin. Invest.

View full text Add to dashboard Cite

Human PMN and monocytes both possess a mechanism for amplifying Fc receptor-mediated phagocytic function, which is dependent on activation of the respiratory burst. The pathway for augmentation of phagocytosis requires superoxide anion, hydrogen peroxide, and lactoferrin and is independent of the hydrogen peroxide-MPO-halide system. In neither cell type is this mechanism induced upon exposure to the opsonized target. PMN require an additional signal for stimulation of the respiratory burst; this is not true of monocytes. On the other hand, monocytes require an exogenous source of lactoferrin in order to activate this pathway for enhanced ingestion. The dependence of this pathway for both PMN and monocytes on superoxide anion, hydrogen peroxide, and cell-bound lactoferrin is consistent with a role for locally generated reactive oxygen metabolites, possibly hydroxyl radicals, in phagocytosis amplification. Patients with chronic granulomatous disease, who are genetically deficient in the ability to activate the respiratory burst, are unable to amplify Fc receptor-mediated phagocytosis. Thus, these patients may have a previously unrecognized defect in the recruitment of phagocytic function at inflammatory sites.

show abstract

“…27 A second effect of amphotericin B is thought to be cell damage secondary to drug autooxidation. 33 …”

Section: Mode Of Actionmentioning

confidence: 99%

Systemic Antifungal Therapy: Past, Present and Future

Al-Mohsen

Hughes

1998

Ann Saudi Med

View full text Add to dashboard Cite

Amphotericin B-Induced Oxidative Damage and Killing of Candida albicans

Cited by 259 publications

References 20 publications

Fluconazole and amphotericin-B resistance are associated with increased catalase and superoxide dismutase activity in Candida albicans and Candida dubliniensis

Fluconazole and amphotericin-B resistance are associated with increased catalase and superoxide dismutase activity in Candida albicans and Candida dubliniensis

Studies on the molecular mechanisms of human Fc receptor-mediated phagocytosis. Amplification of ingestion is dependent on the generation of reactive oxygen metabolites and is deficient in polymorphonuclear leukocytes from patients with chronic granulomatous disease.

Systemic Antifungal Therapy: Past, Present and Future

Contact Info

Product

Resources

About