Fluconazole and amphotericin-B resistance are associated with increased catalase and superoxide dismutase activity in Candida albicans and Candida dubliniensis

Linares, Carlos Eduardo Blanco; Giacomelli, Sandro Rogério; Altenhofen, Delsi; Alves, Sydney Hartz; Morsch, Vera Maria; Schetinger, Maria Rosa Chitolina

doi:10.1590/0037-8682-0190-2013

Cited by 44 publications

(41 citation statements)

References 35 publications

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“…Given that different strains had different antioxidant capacity, mainly reflected in the activities of antioxidant enzymes, we suggested that the T. asahii clinical group stains may have higher antioxidant capacity than the environment group strains. According to previous studies, we also obtained that the mean SOD activity of Clinical group strains of T. asahii (10.45 ± 3.87 U/mg) was equivalent to that of Cryptococcus (12 ± 0.5 U/mg) [16], and below that of Candida dubliniensis (27.87 ± 20.82 U/mg) [15], significantly lower than that of C. albicans (151.8 ± 73.27 U/mg) [15]. The mean catalase activity of T. asahi Clinical group strains (48.51 ± 7.60 U/ mg) was significantly higher than that of A. fumigatus (1.75 ± 0.75 U/mg), Aspergillus terreus (3.5 ± 0.8 U/mg) [17].…”

Section: Discussionsupporting

confidence: 62%

“…At the 9th generation of reply induction, the mean SOD activity of strains was 3.53 ± 0.84 U/mg, the mean catalase activity was 47.15 ± 25.88 U/mg. These results implied that fluconazole may simulate or induce the expression of antioxidant enzymatic activity of T. asahii, leaving the antioxidant capacity of these strains further promoted [15]. Because that the activities of SOD and catalase in T. asahii could go down to normal level without fluconazole exposure, we suggested that the induction of antioxidant enzymatic activity may be regulatory [1].…”

Section: Discussionmentioning

confidence: 82%

“…Because that the activities of SOD and catalase in T. asahii could go down to normal level without fluconazole exposure, we suggested that the induction of antioxidant enzymatic activity may be regulatory [1]. There were other antifungal agents that also could induce antioxidant stress response except fluconazole and amphotericin B [15]. Hoehamer et al [2] confirmed that the expression of the proteins involved in antioxidant mechanism elevated after the exposure of the azole (ketoconazole) and echinocandin [17] pointed out that Aspergillus terreus who had inherent resistance to amphotericin B was closely related with its higher catalase activity and had no obvious correlation to its cell wall components and lipid peroxidation level when compared with the A. fumigatus.…”

Section: Discussionmentioning

confidence: 93%

“…The preparation was employed based on the assay of Linares et al [15]. The strains were cultured on PDA for 48 h at 35°C.…”

Section: Fungal Cells and Enzyme Extracts Preparationmentioning

confidence: 99%

“…They were resuspended by potassium phosphate buffer (PH 7.0, 50 mmol/l), and added 0.5 g glass beads (Sigma, USA). Before the centrifugation, the suspensions above were shaking strongly for 6 times, then the supernatant was collected for the enzymatic assays [15].…”

Section: Fungal Cells and Enzyme Extracts Preparationmentioning

confidence: 99%

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Study on Antioxidant Enzymatic Activities of Trichosporon asahii

Zhang

Wang

2016

Indian J Microbiol

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Superoxide dismutase (SOD) and catalase are considered the most important antioxidant enzymes which protect fungus from the oxidant damage of reactive oxygen species. In this study, we collected 44 strains of () from different sources and investigated their SOD and catalase activities. The results showed that the SOD and catalase activities of Clinical group were significantly higher than those of Environment group ( < 0.01). The SOD and catalase activities of in Internal passage group went up gradually after passage in mice, and were significantly higher in 5th generation of Internal passage group ( < 0.05). The SOD and catalase activities of Fluconazole-resistant group strains also increased after resistant induction, and the SOD and catalase activities were significantly higher in the 10th generation of Fluconazole-resistant group ( < 0.05). This implied that has stronger antioxidant ability. The strains of from different sources have different antioxidant abilities, which mainly manifest in the difference of antioxidant enzymatic activities. Clinical group strains have the strongest antioxidant capacity; Internal passage group strains and Fluconazole resistant group strains better; Environmental group strains the lowest. These results also suggested that the antioxidant defensive response of might be relevant to its infection mechanism and drug resistance mechanism.

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Section: Discussionsupporting

confidence: 62%

Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 93%

“…The preparation was employed based on the assay of Linares et al [15]. The strains were cultured on PDA for 48 h at 35°C.…”

Section: Fungal Cells and Enzyme Extracts Preparationmentioning

confidence: 99%

Section: Fungal Cells and Enzyme Extracts Preparationmentioning

confidence: 99%

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Study on Antioxidant Enzymatic Activities of Trichosporon asahii

Zhang

Wang

2016

Indian J Microbiol

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Photoinactivation of Catalase Sensitizes Candida albicans and Candida auris to ROS‐Producing Agents and Immune Cells

et al. 2022

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Microbes have developed their own specific strategies to cope with reactive oxygen species (ROS). Catalase, a heme-containing tetramer expressed in a broad range of aerobic fungi, shows remarkable efficiency in degrading hydrogen peroxide (H 2 O 2 ) for fungal survival and host invasion. Here, it is demonstrated that catalase inactivation by blue light renders fungal cells highly susceptible to ROS attack. To confirm catalase as a major molecular target of blue light, wild type Candida albicans are systematically compared with a catalase-deficient mutant strain regarding their susceptibility to ROS through 410 nm treatment. Upon testing a wide range of fungal species, it is found that intracellular catalase can be effectively and universally inactivated by 410 nm blue light. It is also found that photoinactivation of catalase in combination with ROS-generating agents is highly effective in total eradication of various fungal species, including multiple Candida auris strains, the causative agent of the global fungal epidemic. In addition, photoinactivation of catalase is shown to facilitate macrophage killing of intracellular Candida albicans. The antifungal efficacy of catalase photoinactivation is further validated using a C. albicans-induced mouse model of skin abrasion. Taken together, the findings offer a novel catalase-photoinactivation approach to address multidrug-resistant Candida infections.

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Modulation of antioxidant defence system in response to berberine in Candida albicans

Poopedi

Marimani

Alomar

et al. 2020

Yeast

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Emergence of multidrug resistant species of Candida is evolving, which advocates an urgent need for the development of new therapeutic strategies and antifungal drugs. Activation of antioxidant defence system in Candida albicans is known as forefront mechanism to escape drug toxicity. This study evaluated the role of antioxidant defence genes in the susceptibility to fluconazole in C. albicans and also determined the effect of berberine on growth, antioxidant enzymes and the expression of their genes in C. albicans isolates. Expression of major antioxidant genes was significantly increased in fluconazole-resistant isolates in comparison with the susceptible group. Antifungal susceptibility against berberine showed MIC values ranging from 125 to 500 μg/ml. Berberine treatment caused upregulation of mRNA expression and enzymatic activities of the targeted major antioxidants. Interestingly, C. albicans exhibited efficient antioxidant response at lower concentrations but could not sufficiently alleviate berberine-induced oxidative stress occurring at concentrations greater than 250 μg/ml. Therefore, berberine could serve as a potent Reactive Oxygen Species (ROS)-inducing agent, disrupting the antioxidant system especially in fluconazoleresistant C. albicans to overcome antifungal drug resistance. Take Aways • Evaluated the role of antioxidant enzymes in FLC resistance in C. albicans • Studied the effect of berberine on growth of different C. albicans isolates • Investigated the modulation of antioxidant enzymes by berberine in C. albicans • Studied the effect of berberine on antioxidant gene expression in C. albicans

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Fluconazole and amphotericin-B resistance are associated with increased catalase and superoxide dismutase activity in Candida albicans and Candida dubliniensis

Cited by 44 publications

References 35 publications

Study on Antioxidant Enzymatic Activities of Trichosporon asahii

Study on Antioxidant Enzymatic Activities of Trichosporon asahii

Photoinactivation of Catalase Sensitizes Candida albicans and Candida auris to ROS‐Producing Agents and Immune Cells

Modulation of antioxidant defence system in response to berberine in Candida albicans

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