Amphotericin B and 5-Fluorocytosine: In Vitro Effects on Lymphocyte Function

Roselle, Gary A.; Kauffman, Carol A.

doi:10.1128/aac.14.3.398

Cited by 15 publications

(5 citation statements)

References 13 publications

(10 reference statements)

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“…Stewart et al (21) (27). Roselle and Kauffman (19) found that human antigen-specific T cells stimulated by antigen were more sensitive to Fungizone than lymphocytes stimulated by mitogen were, although much higher concentrations were required to inhibit proliferation of both than were required in our study. However, Tamrvik and Ansehn (24) Regardless of the reason for the differences in the effects on the fresh lymphocytes stimulated by specific antigen and the line cells, the former may be more relevant to the immunosuppressive effects in vivo.…”

Section: Discussioncontrasting

confidence: 55%

“…Similar results were obtained for an anti-KLH cell line and an anti-MBP cell line. These concentrations were similar to those of Fungizone and liposomal AMB (MLVs and SUVs) required to increase cation release from erythrocytes, affect macrophage properties, and inhibit proliferation of a myeloma cell line and of lymphocytes in response to mitogens (6,11,12,18,19,21,22,24,27).…”

mentioning

confidence: 54%

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Liposomal amphotericin B inhibits in vitro T-lymphocyte response to antigen

Boggs

Chang

Goundalkar

1991

Antimicrob Agents Chemother

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The effects of free amphotericin B (as Fungizone) and amphotericin B (AMB) incorporated into liposomes on the proliferation of lymphocytes were determined. Freshly obtained guinea pig and rat antigen-specific lymphocytes were compared with rat T-lymphocyte cell lines cultured for a long period of time. Incorporation of AMB into multilayered vesicles significantly reduced its effect relative to that of Fungizone on cultured T-cell lines, as reported by others for mammalian cells. In contrast, the effects on freshly obtained antigen-specific lymphocytes were different. Fungizone inhibited proliferation of antigen-specific lymph node cells freshly obtained from immunized guinea pigs at fungicidal concentrations, and incorporation into multilayered lipid vesicles did not have much of a protective effect. Higher concentrations of Fungizone were required to inhibit proliferation of fresh rat lymph node cells, but incorporation into multilayered lipid vesicles still did not have much of a protective effect. Some T lymphocytes in the peripheral circulation of guinea pigs and in the lymph nodes of rats were more resistant to liposomal AMB than another more sensitive T-lymphocyte population was. Proliferation of lymphocytes in response to mitogens was inhibited less than that in response to specific antigen was. Thus, sensitivity to AMB depended on the species, the strength of the stimulus used to activate the lymphocytes, and on some other property of the lymphocytes, possibly their state of differentiation. Regardless of the reason for the difference in effects on freshly obtained lymph node lymphocytes and cultured line cells, the former may be more relevant to effects in vivo and should be considered in a complete evaluation of the in vivo toxicity of these forms of the drug. Incorporation into sonicated unilamellar vesicles had more of a protective effect, while equimolar drug-lipid complexes had even more of a protective effect. These forms of AMB might have less of an immunosuppressive potential than multilayered vesicles containing low amounts of AMB do.

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Section: Discussioncontrasting

confidence: 55%

mentioning

confidence: 54%

Liposomal amphotericin B inhibits in vitro T-lymphocyte response to antigen

Boggs

Chang

Goundalkar

1991

Antimicrob Agents Chemother

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“…In vitro studies on progenitor bone marrow cells shows that amphotericin B suppresses growth (Meeker et al 1983), but in studies at our institution low dose amphotericin B (0.1 mg/kg/day) had no influence on time to bone marrow engraftment (Perfect et al 1991). Other in vitro and animal models suggest that amphotericin B may suppress human lymphocyte function (Roselle & Kauffman 1978), but also increase the number of antibody-producing cells, and generally enhance cell-mediated immunity through the phagocytic and cidal mechanisms of macrophages (Kucers & Bennett 1987;. Thrombocytopenia has also been rarely reported (Chan et al 1982;Charak et al 1990;Kulpa et al 1981).…”

Section: Haematological Effectsmentioning

confidence: 62%

Adverse Drug Reactions to Systemic Antifungals Prevention and Management

1992

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Systemic administration of antifungal agents for invasive mycoses has dramatically increased over the past 10 years in many fields of medicine. The increase has been due both to an increasing immune compromised population and to potent antibacterial agents which allow these fungi to invade tissue. It is apparent that our understanding of the use of both the old and new antifungal agents has significantly increased in the last few years. In this review, we attempt to document our extensive knowledge of the adverse effects of the polyenes, flucytosine, griseofulvin and azoles when given systemically for treatment. Interwoven in this documentation of the adverse reactions to these agents is the attempt to help clinicians potentially avoid some of these adverse effects and if they do occur, to be able to identify and successfully manage them.

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“…In contrast to these positive results on murine cells, mitogen-and antigeninduced stimulation of human lymphocytes has been found to be suppressed by AmB in vitro [4,128,154]. Human peripheral blood lymphocytes showed a decreased "natural killer" activity in the presence of AmB [lll].…”

Section: Modulation Of Immune Defensesmentioning

confidence: 99%

Mechanism of Action of an Anti-Candida Drug: Amphotericin B and Its Derivatives

Bolard

1991

Candida Albicans

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Amphotericin B and 5-Fluorocytosine: In Vitro Effects on Lymphocyte Function

Cited by 15 publications

References 13 publications

Liposomal amphotericin B inhibits in vitro T-lymphocyte response to antigen

Liposomal amphotericin B inhibits in vitro T-lymphocyte response to antigen

Adverse Drug Reactions to Systemic Antifungals Prevention and Management

Mechanism of Action of an Anti-Candida Drug: Amphotericin B and Its Derivatives

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