Amphoteric natural starch-coated polymer nanoparticles with excellent protein corona-free and targeting properties

Huang, Bo; Yang, Zhenxing; Fang, Shumin; Li, Yan; Zhong, Zicheng; Zheng, Ruoqian; Zhang, Jinzhi; Wang, Hang‐Xing; Wang, Suxiao; Zou, Qichao; Wu, Limin

doi:10.1039/c9nr09405a

Cited by 25 publications

(18 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Starch NPs (St NPs) were previously reported by our group. , To modify the nanoparticles by proteins, the St NPs were diluted to a concentration of 5 mg mL –1 in MES buffer, followed by the addition of 20.0 mg (0.09 mmol) of N -hydroxysuccinimide (NHS) and 10.0 mg (0.05 mmol) of 1-ethyl-3-(3-(dimethylamino)propyl)-carbodiimide (EDC), and then shaken at 250 rpm and 37 °C for 0.5 h. After, the reaction mixture was washed three times under 10 000 rpm centrifugation. Ten mg of casein, soybean protein isolate (SPI), zein, myglobin, and gelatin was separately dissolved in 1.5 mL of PBS buffer, and 0.15 mL of the protein solution was added dropwise to the prepared, activated St NP solution.…”

Section: Methodsmentioning

confidence: 99%

“…The sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS PAGE) method was also used. A similar method was applied as in the literature …”

Section: Methodsmentioning

confidence: 99%

“…The sample preparation and measurement were followed as in a the method in the literature . To quantify the amount of protein corona on each NP, the protein concentration per unit volume of each sample was measured by the Western Blot method and the number of NPs per unit volume for each sample was measured by NanoAnalyzer analysis.…”

Section: Methodsmentioning

confidence: 99%

“…The cell membrane of red blood cells is the first and most wildly used delivery vehicle for cell membrane coated NPs with good antifouling properties to escape the reticuloendothelial system. , However, they hardly target cancer cells due to the lack of homophilic cell adhesion molecules unless they experience some chemical or nondisruptive lipid insertion, , and some stability issues still exist . We previously reported amphoteric starch-coated polymer NPs with superior antifouling and high targeting capabilities, but the protein adsorption on NPs has not been precisely investigated …”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Quantitative Analysis of Protein Corona on Precoated Protein Nanoparticles and Determined Nanoparticles with Ultralow Protein Corona and Efficient Targeting in Vivo

Zhong

Fang

et al. 2021

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

The protein corona on nanoparticles (NPs) is a critical problem that often screens the targeting molecules and becomes one of the key reasons for the lack of practical application in nanotherapy. It is critical to fully understand the mechanism of the nanoparticle–biological interactions to design the nanoparticle-based therapeutic agents. Some types of proteins can be precoated on the nanoparticles to avoid unwanted protein attachment; however, the ultralow level of protein corona is hard to achieve, and the relationship of the antifouling property of the precoated protein nanoparticles with protein conformation and protein–nanoparticle interaction energy has never been investigated. In this work, we provided the quantitative protein corona composition analysis on different precoated protein nanoparticles, and on the basis of the molecular simulation process, we found their antifouling property strongly depended on the interaction energy of the precoated protein–serum protein pair and the number of hydrogen bonds formed between them. Furthermore, it also depended on the nanoparticle–serum protein pair interaction energy and the protein conformation on the nanoparticle. The casein coated nanoparticle with the antifouling property was determined, and after aptamer conjugation and drug loading, they exhibited superior targeting and internalization behavior for photodynamic and photothermal therapy in vitro and in vivo. Our work adds to the understanding of the protein corona behavior of precoated protein nanoparticles, and the determined antifouling NP can potentially be used as a highly efficient nanodrug carrier.

show abstract

Section: Methodsmentioning

confidence: 99%

“…The sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS PAGE) method was also used. A similar method was applied as in the literature …”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Quantitative Analysis of Protein Corona on Precoated Protein Nanoparticles and Determined Nanoparticles with Ultralow Protein Corona and Efficient Targeting in Vivo

Zhong

Fang

et al. 2021

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

show abstract

“…[ 23 ] Other surface coatings and functionalizations that have been shown to affect BC‐related processes include glucose and polyethylene glycol, [ 78 ] polyvinylpyrrolidone (PVP) and poly(2‐vinyl pyridine)‐ b ‐poly(ethylene oxide) (PEO‐ b ‐P2VP), [ 79 ] zwitterionic moieties like cysteine, [ 27 ] a mixture of amine and zwitterionic moieties, [ 14 ] polyglycerol, [ 80 ] short‐chain ethylene oxide (EO) oligomers, [ 81 ] poly(phosphoester)s, [ 82 ] and starch. [ 83 ] Another BC influencing parameter that can be associated with the administration of nanostructures, but that is not an intrinsic property of NPs themselves, is the presence of stabilizing surfactants. For example, the presence of cetyltrimethylammonium chloride together with polystyrene NPs has been shown to affect the composition of the BC formed on the surface of these nanostructures when exposed to plasma.…”

Section: Biomolecular Corona: Significance Characteristics and Exploi...mentioning

confidence: 99%

Biomolecular Corona Associated with Nanostructures: The Potentially Disruptive Role of Raman Microscopy

Battaglini

Gümüş

Ciofani

2021

Adv Materials Technologies

View full text Add to dashboard Cite

investigating the use of nanomaterials within living organisms. When a nanoparticle (NP) encounters biological molecules such as proteins, lipids, nucleic acids, or polysaccharides, it tends to adsorb these molecules on its surface due to electrostatic, hydrophobic, or other forms of interactions between the molecules and the surface of the NP. [1][2][3][4] Depending on their abundance and affinity, proteins and other molecules may form a hard corona that consists of tightly bounded proteins on the surface, or a soft corona, which indicates a second layer of proteins that are loosely attached to the proteins of the hard corona. [4] The composition of the soft corona changes over time according to the environmental conditions, and so the biological identity of nanostructures is usually, but not always, determined by the hard corona. This can be both advantageous and disadvantageous to the application of NPs. Some studies have focused on preventing biomolecular corona (BC) formation, as this may change the size and surface properties, hinder modifications on the surface, and cause the rapid detection and clearance of NPs by the immune system. [5,6] However, BCs can be exploited or even altered, in order to be made beneficial for targeting purposes as they can enable specific cells to more easily recognize NPs and may lead to a reduction in their toxicity. [7,8] A wide range of techniques have been used in BC studies, such as dynamic light scattering (DLS), [9] sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), mass spectrometry (MS)-based approaches, [10][11][12][13][14][15][16][17][18][19][20][21] UV/vis spectroscopy, Fourier-transform infrared spectroscopy (FTIR), [22] atomic force microscopy (AFM), [23] scanning electron microscopy (SEM), [24][25][26][27] transmission electronic microscopy (TEM), [23] and circular dichroism spectroscopy (CD). [28] Various information (size, surface properties, morphology, structure, etc.) about BCs can be obtained through these methods. Many techniques have been applied in BC studies, which can be divided into microscopybased approaches that use direct imaging of the sample, and indirect techniques. Microscopy techniques can provide information about corona composition and shape with a spatial resolution up to the dimension scale of single protein molecules, but they are limited by the requirement for staining or extensive sample preparation. Indirect techniques can provide extensive information about BC-nanostructure interactions and properties, but typically have a limited resolution and so cannot analyze the BC at the level of single nanostructures. Other emerging

show abstract

A Smart Nanovector of Cationic Starch Modified Curcumin with Excellent Targeting and Sonodynamic Properties for Effective Therapy of MRSA‐Induced Osteomyelitis

Chen,

Yang,

Mao

et al. 2023

Adv Funct Materials

Self Cite

View full text Add to dashboard Cite

Drugs treatment including antibiotics or herbal medicines cannot cure osteomyelitis effectively accompanied by drug‐resistance bacteria and even amputation after long‐term debridement. Herein, a materioherbological strategy is utilized to construct a smart nanovector of cationic starch (CS) modified curcumin nanoparticles (CS@Cur NPs) for treating methicillin‐resistant Staphylococcus aureus (MRSA) induced osteomyelitis. On the one hand, CS modification can reduce the π–π accumulation of curcumin to endow the nanocomposite with excellent water solubility. On the other hand, the hydroxyl group in the curcumin is hydrogen‐bonded to the carbonyl group in the monomer (methyl methacrylate, MMA) of CS, resulting in a decrease in both the bandgap (between the highest and lowest occupied molecular orbitals, labeled as ΔE) and the S1–T1 energy gap (ΔES‐T) while the spin–orbital coupling (SOC) values increase, which benefits the formation of triplet‐state molecules under ultrasonic irradiation, significantly improving the yields of reactive oxygen species (ROS) capacity. The CS@Cur NPs nanocomposite constructed in this paper produced a large number of ROS synergic targeting effects under continuous 15 min ultrasonic irradiation, the clearance rate of MRSA infection induced osteomyelitis in rats can be as high as 99.93%. This study provides a new therapeutic strategy for the treatment of osteomyelitis induced by pathogenic bacteria.

show abstract

Amphoteric natural starch-coated polymer nanoparticles with excellent protein corona-free and targeting properties

Abstract: Novel amphoteric starch-stabilized nanoparticles exhibit excellent protein corona-free properties and also functionalized by antibodies to achieve excellent targeting and cell internalization capabilities for their use in photodynamic therapy.

Cited by 25 publications

References 56 publications

Quantitative Analysis of Protein Corona on Precoated Protein Nanoparticles and Determined Nanoparticles with Ultralow Protein Corona and Efficient Targeting in Vivo

Quantitative Analysis of Protein Corona on Precoated Protein Nanoparticles and Determined Nanoparticles with Ultralow Protein Corona and Efficient Targeting in Vivo

Biomolecular Corona Associated with Nanostructures: The Potentially Disruptive Role of Raman Microscopy

A Smart Nanovector of Cationic Starch Modified Curcumin with Excellent Targeting and Sonodynamic Properties for Effective Therapy of MRSA‐Induced Osteomyelitis

Contact Info

Product

Resources

About