Amperometric <scp>l</scp>-Lactate Sensor Based on Sol−Gel Processing of an Enzyme-Linked Silicon Alkoxide

Lin, Cheng-Li; Shih, Cheng-Ling; Chau, Lai-Kwan

doi:10.1021/ac061972d

Cited by 42 publications

(20 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The first one involved the use of polymeric additives (e.g., poly(vinyl alcohol), poly(vinyl pyridine), Nafion, poly(styrene sulfonate), chitosan) in the starting sol [28,29] or the resort to organosilane reagents (e.g., trimethoxysilyl-propyl methacrylate) likely to generate threedimensional uniform structures ready for enzyme immobilization [30]. An original recent approach is the covalent linking of the carboxylic acid group of lactate dehydrogenase to the amino group of an organoalkoxysilane precursor via a carbodiimide coupling reaction during the sol-gel deposition process [31]. The second one (promotion of charge transfer) mainly involved the use of additives such as carbon nanotubes [32,33] or titania nanoparticles [34].…”

Section: Introductionmentioning

confidence: 99%

Critical Effect of Polyelectrolytes on the Electrochemical Response of Dehydrogenases Entrapped in Sol‐Gel Thin Films

et al. 2010

View full text Add to dashboard Cite

The influence of polyelectrolyte (PE) additives on the sol-gel encapsulation of dehydrogenases has been evaluated using d-sorbitol dehydrogenase (DSDH) as a model enzyme. Thin sol-gel films were prepared on glassy carbon electrodes (GCE), which were then evaluated with respect to the electrochemical detection of d -sorbitol. DSDH encapsulation in pure silica thin films resulted in undetectable electrochemical signal. Adding positively-charged PE that contributes to stabilize the negatively-charged DSDH in the biocomposite film led to much faster and higher response of DSDH to its substrate, as shown by the significant improvement in bioelectrochemical detection of NADH produced during the oxidation of d-sorbitol.

show abstract

Section: Introductionmentioning

confidence: 99%

Critical Effect of Polyelectrolytes on the Electrochemical Response of Dehydrogenases Entrapped in Sol‐Gel Thin Films

et al. 2010

View full text Add to dashboard Cite

show abstract

“…Для ефективної роботи біосенсора важливим є збереження активності фермента тривалий час за умови його розташуванням в безпосередній близькості до поверхні електрода. Для іммобілізації ферментів часто використовують методи на основі ковалентного зшивання ферментів між собою, або з білковою основою, фізичне включення ферментів в провідні чи непровідні полімери або гідрогель, а також комбінацію цих методів.Також є повідомлення, що для підвищення стабільності ферменту використовували золоті наночастинки, які спільно з лактатоксидазою іммобілізували в тривимірному гелі [10]. Тіольні зв'язки забез-печили фіксацію ферменту на наночастинках золота, а збільшення площі поверхні призвело до збільшення кількості іммобілізованих молекул ферменту.…”

Section: вступunclassified

Development of Amperometric Biosensor Based on Lactate Oxidase for Lactate Determination

Topolnikova¹,

Кучеренко²,

Шкотова³

et al. 2016

SEMST

View full text Add to dashboard Cite

“…Lin et al reported a strategy to combat leaching by covalently linking lactate dehydrogenase to an organoalkoxysilane precursor via a carbodiimide coupling reaction prior to sol-gel processing to form the sensor membrane. 145 The resulting material was coated on a glassy carbon electrode to prepare a lactate biosensor with good long-term operational stability (~1 week).…”

Section: Enzyme Biosensorsmentioning

confidence: 99%

Electrochemical Sensors

2013

Encyclopedia of Biophysics

View full text Add to dashboard Cite

This review covers publications related to electrochemical sensors that appeared in print during the years 2006 and 2007. The focus of the review is on the development of electrochemical sensing principles including potentiometric and chronopotentiometric sensors, reference electrodes, voltammetric sensors, and electrochemical biosensors.References were collected by searching electrochemical sensor-related work using ACS SciFinder and ISI Science Citation Index. In addition, the tables of contents of several analytical journals were reviewed manually to identify relevant articles. Only original and review articles written in English were considered. Patents, book chapters or book series, and conference proceedings or abstracts were not considered for this review. The number of references was limited to 200 and as a result only a fraction of relevant work is covered. Specifically, the review was written to focus on fundamental aspects of electrochemical sensor research emphasizing new chemical concepts and not simply the application of electrochemical sensors. On the basis of these guidelines, several hybrid systems whereby electrochemical detection schemes were combined with a separation technique such as microdialysis, electrophoresis, chromatography, and lab-on-a-chip were not included in this review.We ask that our readers note that our review is not a comprehensive coverage of the above topics, but rather a snapshot of research conducted over the past two years. Obviously, electrochemical sensor research is still expanding and of interest to a number of disciplines beyond traditional chemistry. As such, it was not possible to cover all manuscripts published. We apologize to the authors of manuscripts that were not included in this review. POTENTIOMETRIC SENSORS ReviewsBakker and Pretsch published a broad review on modern potentiometry describing techniques for improving sensor performance, miniaturizing sensor platforms, and expanding the application of potentiometric sensors for environmental trace analysis and biosensing (98 citations). 1 Likewise, Pretsch provided a general overview on ion-selective electrodes (ISEs) focusing on improving detection limits and understanding non-classical (i.e., non-equilibrium) potentiometry for monitoring clinical relevant polyions such as heparin and protamine (81 citations). 2 De Marco et al. reviewed ISE methodologies for the analysis of trace metals and anions (e.g., F − , CN − , NO 3 − , NO 2 − , and Cl − ) in environmental samples. 3

show abstract

Amperometric l-Lactate Sensor Based on Sol−Gel Processing of an Enzyme-Linked Silicon Alkoxide

Cited by 42 publications

References 53 publications

Critical Effect of Polyelectrolytes on the Electrochemical Response of Dehydrogenases Entrapped in Sol‐Gel Thin Films

Critical Effect of Polyelectrolytes on the Electrochemical Response of Dehydrogenases Entrapped in Sol‐Gel Thin Films

Development of Amperometric Biosensor Based on Lactate Oxidase for Lactate Determination

Electrochemical Sensors

Contact Info

Product

Resources

About