Amperometric mediatorless hydrogen peroxide sensor with horseradish peroxidase encapsulated in peptide nanotubes

Feyzizarnagh, Hamid; Park, Byung-Wook; Sharma, Lohit; Patania, Michelle; Yoon, Do Young; Kim, Dong-Shik

doi:10.1016/j.sbsr.2015.12.003

Cited by 14 publications

(5 citation statements)

References 14 publications

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“…Thus, this study utilizes an HRP-tagged antibody as the detection probe that reacts with the H 2 O 2 substrate. However, since the enzyme’s reduction is usually prolonged due to slow electron transfer [ 17 ], HQ is introduced into the system to facilitate the transfer of electrons, resulting in the redox reaction between the electrode surface and the redox center of HRP [ 18 , 19 ]. While there is nothing new about the technique, the performance of the HRP-H 2 O 2 -HQ technique is undeniable.…”

Section: Resultsmentioning

confidence: 99%

Surface-Enhanced Carboxyphenyl Diazonium Functionalized Screen-Printed Carbon Electrode for the Screening of Tuberculosis in Sputum Samples

et al. 2022

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Curbing tuberculosis (TB) requires a combination of good strategies, including a proper prevention measure, diagnosis, and treatment. This study proposes an improvised tuberculosis diagnosis based on an amperometry approach for the sensitive detection of MPT64 antigen in clinical samples. An MPT64 aptamer specific to the target antigen was covalently attached to the carboxyphenyl diazonium-functionalized carbon electrode via carbodiimide chemistry. The electrochemical detection assay was adapted from a sandwich assay format to trap the antigen between the immobilized aptamer and horseradish peroxidase (HRP) tagged polyclonal anti-MPT64 antibody. The amperometric current was measured from the catalytic reaction response between HRP, hydrogen peroxide, and hydroquinone, which is used as an electron mediator. From the analysis, the detection limit in the measurement buffer was 1.11 ng mL−1. Additionally, the developed aptasensor exhibited a linear relationship between the current signal and the MPT64 antigen-spiked serum concentration ranging from 10 to 150 ng mL−1 with a 1.38 ng mL−1 detection limit. Finally, an evaluation using the clinical sputum samples from both TB (+) and TB (−) individuals revealed a sensitivity and specificity of 88% and 100%, respectively. Based on the analysis, the developed aptasensor was found to be simple in its fabrication, sensitive, and allowed for the efficient detection and diagnosis of TB in sputum samples.

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Section: Resultsmentioning

confidence: 99%

Surface-Enhanced Carboxyphenyl Diazonium Functionalized Screen-Printed Carbon Electrode for the Screening of Tuberculosis in Sputum Samples

et al. 2022

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“…Other extensively studied nanoreactor systems are based on peptides or DNA (origami). [186][187][188][189][190] DNA origamis can consist of various shapes such as tube-like honeycomb structures made from DNA that are combined into a large structure. A cavity within these structures can contain enzymes, for example, which are coupled to the DNA origami using a short complementary DNA strand.…”

Section: Advantages Of Protein Nanoreactors Over Other Nanoreactorsmentioning

confidence: 99%

Structural nanotechnology: three-dimensional cryo-EM and its use in the development of nanoplatforms forin vitrocatalysis

et al. 2019

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“…Until now, several sensors have been reported for determining H 2 O 2 in different matrices through enzymatic reactions, using peroxidase reactions such as horseradish peroxidase (HRP). The assayed enzyme catalyzed the oxidation of a wide variety of organic compounds such as 3-ethylbenzothiazoline-6-sulfonic acid (ABTS), N , N -diethyl- p -phenylenediamine (DPD), o -phenylenediamine (OPD), 3,3′-diaminobenzidine (DAB), 3,3′,5,5′-tetramethyl-benzidine (TMB), or luminol. − These methods include both electrochemical ,− and optical techniques. ,− Amplex Red derivatives, instead of luminol and colorimetric substrates, are also applied to quantify hydrogen peroxide in the presence of HRP with fluorescence and 1,1′-oxalyldiimidazole chemiluminescence (CL) detection . In addition, hydrogen peroxide in a sample can be quantified with fluorescent and luminescent probes without HRP. − However, as far as we know, there are no publications in the literature where a portable and reusable HRP sensor is developed.…”

Section: Introductionmentioning

confidence: 99%

New Reusable Solid Biosensor with Covalent Immobilization of the Horseradish Peroxidase Enzyme: In Situ Liberation Studies of Hydrogen Peroxide by Portable Chemiluminescent Determination

2020

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Herein, we reported a chemiluminescent biosensor based on the covalent immobilization of the horseradish peroxidase (HRP) enzyme on a polydimethylsiloxane (PDMS) support to quantify in situ hydrogen peroxide (H 2 O 2 ). The chemiluminescent reaction based on the use of luminol as an oxidizable substrate, with HRP as the catalyst, has been used in order to quantify H 2 O 2 as the oxidizing agent. The performance of the proposed biosensor has been demonstrated to determine H 2 O 2 liberated by cells in a culture medium and for evaluating the delivery of H 2 O 2 from denture cleaner tablets, as examples of application. For both analyses, the results indicated that the biosensor is cost-effective, sensitive, and selective with a detection limit of 0.02 μM and good linearity over the range 0.06−10 μM. Precision was also satisfactory (relative standard deviation, % RSD < 6). The strength of this biosensing system is the simplicity, portability, and reusability of the devices; it can be applied up to 60 times with 90% of its activity maintained.

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Amperometric mediatorless hydrogen peroxide sensor with horseradish peroxidase encapsulated in peptide nanotubes

Cited by 14 publications

References 14 publications

Surface-Enhanced Carboxyphenyl Diazonium Functionalized Screen-Printed Carbon Electrode for the Screening of Tuberculosis in Sputum Samples

Surface-Enhanced Carboxyphenyl Diazonium Functionalized Screen-Printed Carbon Electrode for the Screening of Tuberculosis in Sputum Samples

Structural nanotechnology: three-dimensional cryo-EM and its use in the development of nanoplatforms forin vitrocatalysis

New Reusable Solid Biosensor with Covalent Immobilization of the Horseradish Peroxidase Enzyme: In Situ Liberation Studies of Hydrogen Peroxide by Portable Chemiluminescent Determination

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