Amperometric immunosensors and enzyme electrodes for environmental applications

Parellada, Josefina; Narváez, Arántzazu; López, Miguel Ángel; Domı́nguez, Elena; Fernández, Juan J.; Pavlov, Valeri; Katakis, Ioanis

doi:10.1016/s0003-2670(98)00087-7

Cited by 96 publications

(43 citation statements)

References 26 publications

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“…The flow injection determinations with graphite electrodes with tyrosinase immobilized in a hydrogel correlate satisfactory with the official method for the determination of the phenol index in environmental samples [71]. The peroxidase-modified carbon paste electrode was employed as an amperometric flow injection detector for peroxides in a partial aqueous media [72].…”

Section: Flow Analysis With Voltammetric and Amperometric Detectionsmentioning

confidence: 65%

Electroanalytical Flow Measurements–Recent Advances

2003

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As general trends in current development of chemical analysis, including electroanalysis, one can indicate a search for methods fast and multianalyte, for miniaturized measuring devices, mechanization and automation of analytical processes. In all these trends a significant role is played by measurements in flow conditions. The major advantage of flow electroanalysis is a possibility of utilizing a kinetic discrimination in potentiometric measurements and enhancement of mass transport in voltammetric techniques. Flow injection techniques provide shortening of time of a single analytical determination due to reproducible use of transient signal from the detector without need of obtaining a steady-state equilibrium signal. Electrochemical detection in HPLC gives often improved selectivity and detection limit for electroactive solutes, whereas in capillary electrophoresis it allows a convenient design of portable, integrated chips for field application. This review presents state-of-the -art of flow electroanalysis based on 226 cited literature references

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Section: Flow Analysis With Voltammetric and Amperometric Detectionsmentioning

confidence: 65%

Electroanalytical Flow Measurements–Recent Advances

2003

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“…The analytical performance of these sensors does not demand preconcentration steps and water samples can be injected directly into the automatic system. The obtained results are expressed as biosensor index and can be compared with the official method of phenol index 20 .…”

Section: Universidad De Alcalá De Henaresmentioning

confidence: 99%

Overview of Biosensors Development Around the World

Mongra

Kaur

2012

Int J of Biomed & Adv Res

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Since there are well over 500 companies and research organizations worldwide involved in biosensor development. It is important to note that the major biosensor players were identified in terms of the following criteria: publication output / quality, scientific impact, reputation, and size. The commercialization of biosensor technology has significantly lagged behind the research output. The rationale behind the slow and limited technology transfer could be attributed to cost considerations and some key technical barriers.. Successful biosensors must be versatile to support interchangeable biorecognition elements, and in addition miniaturization must be feasible to allow automation for parallel sensing with ease of operation at a competitive cost. A significant upfront investment in research and development is a prerequisite in the commercialization of biosensors. The progress in such endeavors is incremental with limited success, thus, the market entry for a new venture is very difficult unless a niche product can be developed with a considerable market volume.

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“…A limitação no nú-mero das análises tem criado uma demanda por tecnologias analíticas que possam permitir um aumento no número destas análises num menor tempo e com custos acessíveis [1][2]14,18 . Até o presente momento, análises tanto de fenol como de espécies fenólicas têm sido realizadas, principalmente, por meio de métodos espectrofotométricos e cromatográficos (cromatografia gasosa com ionização em chama ou acoplada com espectrometria de massa; ou ainda, utilizando-se cromatografia *e-mail: kubota@iqm.unicamp.br líquida de alta eficiência) [19][20][21] . Entretanto, estas técnicas não permitem, facilmente, um monitoramento continuo "on site", pois são caras, lentas, necessitam de operadores bem treinados, e em alguns casos, requerem etapas de extração ou pré-concentração, que aumentam o risco de perda de amostra 2,17,22 .…”

Section: Introductionunclassified

“…Os biossensores revelam grandes perspectivas quanto a sua utilização no monitoramento "on-line" de efluentes (e outras matrizes de interesse ambiental), possibilitando uma rápida adaptação nos processos de tratamento. A incorporação de moléculas com atividade biológica em metodologias analíticas tem aumentado sensivelmente nos últimos anos, obtendo sucesso nos mais variados procedimentos analíticos, principalmente nos que visam a área de controle ambiental 5,[14][15][16][17][18][19][20][21][23][24][25][26][27][28][29][30][31][32][33][34][35][36] . Biossensores representam uma ferramenta promissora para suplementar as técnicas existentes, devido as suas características únicas, tais como: seletividade; relativo baixo custo de construção e estocagem; potencial para miniaturização; facilidade de automação e construção de equipamentos simples e portáteis para um monitoramento "on site" rápido.…”

Section: Introductionunclassified

“…Esta gama de configurações confere uma grande versatilidade aos biossensores, que podem ser genéricos, dando uma indicação da presença de um contaminante que tenha algum bioefeito (por exemplo, sensores de toxicidade) ou de um grupo de contaminantes (ex. : pesticidas como inibidores de enzimas); ou ainda especí-ficos, fazendo vantagem da especificidade de uma biomolécula para uma determinada classe ou composto 19 .…”

Section: Introductionunclassified

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