Amperometric glucose sensors based on ferrocene-containing B-polyethylenimine and immobilized glucose oxidase

Chuang, Chen-Lian; Wang, Y.J.; Lan, H.L.

doi:10.1016/s0003-2670(97)00372-3

Cited by 38 publications

(32 citation statements)

References 21 publications

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“…The cyclic voltanmetry showed some evidence for the electron transfer in the electrode coated with 20 nm of DMAMF PPF. 18,19 Compared with similar concepts for strategy with the redox polymers containing polyethyleinmine, 10 poly-L-lysine, 11 and poly-allyamine, 12 our method is thought to be robustness because this is very simple and solventless. The matrix for enzyme immobilization and mediator can be obtained after only 1 min of plasma exposure time; thus, the plasma process is compatible with the micromachining process and is verified to be a simple process for electrode fabrication.…”

Section: Characterization Of Dimethylaminomethylferrocene Plasma Polymentioning

confidence: 99%

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Amperometric Glucose Biosensor Based on Mediated Electron Transfer between Immobilized Glucose Oxidase and Plasma-polymerized Thin Film of Dimethylaminomethylferrocene on Sputtered Gold Electrode

Kase

Muguruma

2004

ANAL. SCI.

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We propose an electron transfer-mediated amperometric enzyme biosensor based on plasma-polymerized thin film of dimethylaminomethylferrocene (DMAMF) on a sputtered gold electrode. The DMAMF plasma-polymerized film is deposited directly onto the surface of the electrode under dry conditions. The resulting thin film not only has redox sites but also is extremely thin (∼20 nm), adheres well onto the substrate (electrode), has a flat surface and a highly-crosslinked network structure, and is hydrophilic in nature. Glucose oxidase is densely immobilized onto the surface of DMAMF plasma-polymerized film on the gold electrode. From the electrochemical measurement, the biosensor can cover the wide range of glucose concentration (1.3 -81 mM) at +350 mV of applied potential. The current response of the glucose biosensor was decreased by less than 5% in an aerobic solution as compared to that in an anaerobic solution. These show that the DMAMF plasma-polymerized films play a role as the electron transfer mediators between the reaction center of enzyme and the electrode.

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Section: Characterization Of Dimethylaminomethylferrocene Plasma Polymentioning

confidence: 99%

“…A ferrocene is a typical mediator for enzyme biosensors. [1][2][3][4][5][6][7][8][9][10][11][12] Usually, the enzyme and mediators are embedded or immobilized in/onto a cast polymer on an electrode surface. The integrated design for sensor device between enzyme and redox site strongly influences the sensor characteristics.…”

Section: Introductionmentioning

confidence: 99%

Amperometric Glucose Biosensor Based on Mediated Electron Transfer between Immobilized Glucose Oxidase and Plasma-polymerized Thin Film of Dimethylaminomethylferrocene on Sputtered Gold Electrode

Kase

Muguruma

2004

ANAL. SCI.

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“…The recorded current will then be proportional to the glucose concentration in the sample. Previously it has been clearly shown that using polymeric bound mediators and especially polymeric mediators covalently bound with the enzyme is beneficial to obtain stable biosensors [41][42][43][44][45][46][47][48][49][50][51][52][53][54][55][56][57][58][59][60]. However, in the case stipulated in this work when coimmobilising GOD and HRP it has been possible to achieve long term stable sensors.…”

Section: Resultsmentioning

confidence: 98%

The synergetIc effect of redox mediators and peroxidase in a bienzymatic biosensor for glucose assays in FIA

Rondeau

Larsson²,

Boujtita

et al. 1999

Analusis

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Amperometric biosensors based on enzymes are interesting due to their high sensitivity, excellent selectivity, simplicity, low cost and rapid response. The most frequently used enzymatic methods for glucose determination employ glucose oxidase (GOD), due to its high selectivity towards ß-D-glucose. In the presence of GOD, the oxidation of glucose occurs, producing H 2 O 2 . Most commonly, the electrochemical detection is performed through the anodic oxidation of H 2 O 2 [1]. A drawback with this detection technique is that high overvoltages are required, and an applied potential in the range of 0.6-1.1 V vs. Ag/AgCl is necessary. Working at such high potentials increases the risk of interference from easily oxidisable compounds such as ascorbate, ureate, acetaminophen, paracetamol, phenols, etc. which are common compounds in biological samples.A possible way of improving the selectivity of such a biosensor is to couple a second enzyme (peroxidase) to the oxidase, creating a bienzymatic sensor. Peroxidase catalyses the electrochemical reduction of H 2 O 2 produced by GOD through a direct electron transfer reaction at a much lower applied potential compared with electrochemical oxidation of H 2 O 2 and maximum catalytic current can be obtained at carbon electrodes in a potential range of -0.1 and 0 V [2-14]. However, the kinetics of the direct electron transfer from an electrode to the peroxidase is a sluggish process [15][16][17] hence the advantage of using a mediator.The introduction of a mediator in the bienzymatic system (GOD/HRP) leads to an acceleration of the electron transfer, a decrease in the applied working potential (the reduction of the mediator is detected at the electrode) and an increase in sensitivity. The use of mediators is a well-known technique although the way of adding the mediator to the bienzymatic system (oxidase/peroxidase) may vary. A number of different mediators have been used as additions into sample solutions such as, potassium hexacyanoferrate (II) [18][19][20][21][22][23][24] [44,45], and Nafion-Nmethyl phenazinium [46]. The advantages of using the redox polymers are several, but mainly they result in more stable biosensors since leaking of the mediator from the electrode is minimised and higher and faster responses are observed due to the proximity between the enzyme and the mediator. Although, choosing an appropriate bulk matrix and mediator concentration these properties can be achieved with nonpolymeric mediators and recently a ferrocene mediated bienzymatic graphite-Teflon electrode has been developed for batch and flow-injection measurements [47]. On the other hand several biosensors based on monoenzymatic electrodes 649The synergetIc effect of redox mediators and peroxidase in a bienzymatic biosensor for glucose assays in FIA Abstract. A bienzymatic biosensor incorporating a mediator has been developed in order to achieve a fast and selective detection of glucose in a flow injection system. The working electrode is based on a carbon paste matrix bulk modified with glucose ...

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“…Biosensors are devices that have the ability to do this. Specifically, enzyme-based biosensors are of great importance for their ability to detect important biological molecules (Shen et al 2017;Nakabayashi et al 1998;Chuang et al 1997;Chi and Dong 1993;Creager and Olsen 1995;Shigehara et al 1981;Battaglini et al 1999;Dong et al 1991;Janarthanan and Mottola 1998;Ohara et al 1994;Mafatle and Nyokong 1997). Some of these biosensors are based on using oxygen as a redox mediator and then detecting the by-product hydrogen peroxide.…”

Section: Introductionmentioning

confidence: 99%

“…The process of immobilizing a redox mediator into a hydrogel requires approximately 48 hours to complete (Battaglini et al 1999), whereas in our electrode modification process, the time is shortened to 6-8 hours, and the electrode is ready to use in one day; electropolymerization of the redox mediator described in this paper takes several minutes to complete. Preparation of a biosensor using ferrocene and an enzyme in a hydrogel matrix is complex and requires more than eight hours to cure the film under vacuum (Chuang, et al, 1997). The goal in this research was to develop a process in designing a biosensor within several hours as opposed to several days.…”

Section: Introductionmentioning

confidence: 99%

Development and Optimization of a Glucose Biosensor Based on Tris [5-amino-1,10-Phenanthroline]Iron(II) Polymer Films

Brown¹,

Cushman²

2018

IJC

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A bilayered glucose biosensor consisting of tris[5-amino-1, 10-phenanthroline]iron(II) polymer film redox mediator and a glucose oxidase layer was prepared on glassy carbon surfaces. The polymer film of the iron complex was immobilized onto the electrode using cyclic voltammetry via electropolymerization reactions, while the enzyme layer was formed using a BSA and glutaraldehyde crosslinking reaction. The biosensors gave the largest response in the pH range of 7-8 and were evaluated with respect to storage conditions of room temperature and 4 o C. There was no significant difference between the detection of glucose using the biosensor stored at room temperature versus one stored at 4°C and both bilayered films remained active for 20 days. The detection limit of the biosensors was found to be 0.30 mM which corresponds to a signal to noise ratio of 3:1.

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Amperometric glucose sensors based on ferrocene-containing B-polyethylenimine and immobilized glucose oxidase

Cited by 38 publications

References 21 publications

Amperometric Glucose Biosensor Based on Mediated Electron Transfer between Immobilized Glucose Oxidase and Plasma-polymerized Thin Film of Dimethylaminomethylferrocene on Sputtered Gold Electrode

Amperometric Glucose Biosensor Based on Mediated Electron Transfer between Immobilized Glucose Oxidase and Plasma-polymerized Thin Film of Dimethylaminomethylferrocene on Sputtered Gold Electrode

The synergetIc effect of redox mediators and peroxidase in a bienzymatic biosensor for glucose assays in FIA

Development and Optimization of a Glucose Biosensor Based on Tris [5-amino-1,10-Phenanthroline]Iron(II) Polymer Films

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