Amperometric Glucose Enzyme Sensor Prepared by Immobilizing Glucose Oxidase on CuPtCl6 Chemically Modified Electrode

Abstract

A simple, sensitive, stable and low-cost glucose sensor with a fast response that operates under aerobic solution conditions is described. The electrode consisted of a CuPtCl 6 modi®ed ®lm deposited on the surface of the glassy carbon electrode onto which the glucose oxidase was immobilized via cross-linking of glutaraldehyde in the presence of bovine serum albumin (BSA). The electropolymerized CuPtCl 6 ®lm was prepared by continuously cyclically scanning between 700 mV and À800 mV (vs. SCE) in a solution containing CuCl 2 and K 2 PtCl 6 . The preparation and the performance of the enzyme electrode have been discussed. The effects of enzyme loading, pH, and the temperature have been studied. The experiments on interference and speci®city have also been demonstrated. The enzyme electrode exhibits an excellent biocatalytic activity and stability. The enzyme electrode provides a linear response to glucose over a concentration range of 2610 À6 M to 1610 À3 M with a detection limit of 1610 À6 M.

Improvement in glucose biosensing response of electrochemically grown polypyrrole nanotubes by incorporating crosslinked glucose oxidase

“…Therefore use of some other immobilization techniques, incorporating enzymes in CP matrix with stronger binding forces is highly desired. In this regard, cross-linking has emerged out to be a powerful technique for enzyme immobilization [24].…”

“…In contrast, immobilization by cross linking of enzyme with the support matrix via some bi-functional cross linking agent such as glutaraldehyde is a separate process similar to physical adsorption and a relatively small amount of enzyme is required as compared to co-entrapment. At the same time sensitivity and storage stability of the biosensor can be extended up to several times [24]. …”

Fluorescent nano-PEBBLE sensors designed for intracellular glucose imaging

“…The development of sensitive, reproducible and stable sensors for monitoring physiological levels of glucose in blood is highly desirable, primarily due to the link with diabetes mellitus and the commercial ramifications of designing the perfect glucose monitoring system. [6][7][8][9][10][11][12][13][14][15] An area of interest that has remained under-represented in the literature is the direct measurement of cellular glucose levels, probably due to a lack of methodologies and instrumentations for making these measurements. Some recent work by Jung et al 16,17 describes progress in measuring extracellular glucose levels, using an electrochemical glucose microsensor to measure glucose fluctuations in the extracellular space of single islets of Langerhans, or the glucose consumption by pancreatic b-cells.…”

Optimal Environment for Glucose Oxidase in Perfluorosulfonated Ionomer Membranes: Improvement of First-Generation Biosensors

“…High sensitivities to glucose could be estimated from the calibration plots reported for the biosensors based on platinized glassy carbon, , referred to as a “porous platinum particle matrix” . However, the sensitivities are lower compared to our enzyme electrode, if the relationship between steady state and flow injection assays is taken into account and especially if recalculated relative to the enzyme concentration.…”

“…However, the sensitivities are lower compared to our enzyme electrode, if the relationship between steady state and flow injection assays is taken into account and especially if recalculated relative to the enzyme concentration. Moreover, the rough transducer surface of the experiments of refs and does not allow glucose sensing in the submicromolar range even in batch mode. Even taking the calculated detection limits 38,39 rather than the really achieved LLD, we obtain for enzyme electrodes 38,39 a newly introduced parameter in the frame of (0.5−1) × 10 5 A M -2 cm -2 , which is 1 order of magnitude lower than that for the developed biosensor (Table ).…”