Amperometric glucose biosensor based on glucose oxidase immobilized over chitosan nanoparticles from gladius of Uroteuthis duvauceli

Anusha, J.R.; Raj, C. Justin; Cho, Bo-Bae; Fleming, Albin T.; Kim, Byung Chul

doi:10.1016/j.snb.2015.03.110

Cited by 68 publications

(29 citation statements)

References 50 publications

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“…An efficient and sensitive glucose biosensor with good sensitivity can be fabricated by immobilizing GOx with an appropriate electrochemical transducer212429303132. Physical adsorption (drop casting method) is the simplest of all the immobilizing techniques to incorporate enzymes to transducers without affecting their native conformation, and this technique is commonly used for biosensors in the research stage33.…”

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confidence: 99%

Novel amperometric glucose biosensor based on MXene nanocomposite

Rakhi

Nayak

Xia

et al. 2016

Sci Rep

304

187

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A biosensor platform based on Au/MXene nanocomposite for sensitive enzymatic glucose detection is reported. The biosensor leverages the unique electrocatalytic properties and synergistic effects between Au nanoparticles and MXene sheets. An amperometric glucose biosensor is fabricated by the immobilization of glucose oxidase (GOx) enzyme on Nafion solubilized Au/ MXene nanocomposite over glassy carbon electrode (GCE). The biomediated Au nanoparticles play a significant role in facilitating the electron exchange between the electroactive center of GOx and the electrode. The GOx/Au/MXene/Nafion/GCE biosensor electrode displayed a linear amperometric response in the glucose concentration range from 0.1 to 18 mM with a relatively high sensitivity of 4.2 μAmM−1 cm−2 and a detection limit of 5.9 μM (S/N = 3). Furthermore, the biosensor exhibited excellent stability, reproducibility and repeatability. Therefore, the Au/MXene nanocomposite reported in this work is a potential candidate as an electrochemical transducer in electrochemical biosensors.

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mentioning

confidence: 99%

Novel amperometric glucose biosensor based on MXene nanocomposite

Rakhi

Nayak

Xia

et al. 2016

Sci Rep

304

187

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“…[1c] Recently, a glucose biosensor constructed of GOx immobilized on chitosan nanoparticles on gold was described that exhibits a response time similar to our biosensor of ≤2 s, yet provides a higher sensitivity of 156.27 μA mM −1 cm −2 and a lower detection limit of 1.1 μM. [22] However, no selectivity data was given, which is an important consideration for sensors to be used in vivo or with biological samples. Another recent review describes the impressive performance characteristics of a number of glucose biosensors based on nanostructured metal oxides including some amperometric electroenzymatic biosensors with several-fold higher sensitivity than our biosensor, yet none exhibit a response time of 2 s or less.…”

Section: Resultsmentioning

confidence: 99%

Enzyme Deposition by Polydimethylsiloxane Stamping for Biosensor Fabrication

2017

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High-performance biosensors were fabricated by efficiently transferring enzyme onto Pt electrode surfaces using a polydimethylsiloxane (PDMS) stamp. Polypyrrole and Nafion were coated first on the electrode surface to act as permselective films for exclusion of both anionic and cationic electrooxidizable interfering compounds. A chitosan film then was electrochemically deposited to serve as an adhesive layer for enzyme immobilization. Glucose oxidase (GOx) was selected as a model enzyme for construction of a glucose biosensor, and a mixture of GOx and bovine serum albumin was stamped onto the chitosan-coated surface and subsequently crosslinked using glutaraldehyde vapor. For the optimized fabrication process, the biosensor exhibited excellent performance characteristics including a linear range up to 2 mM with sensitivity of 29.4 ± 1.3 μA mM−1 cm−2 and detection limit of 4.3 ± 1.7 μM (S/N = 3) as well as a rapid response time of ~2 s. In comparison to those previously described, this glucose biosensor exhibits an excellent combination of high sensitivity, low detection limit, rapid response time, and good selectivity. Thus, these results support the use of PDMS stamping as an effective enzyme deposition method for electroenzymatic biosensor fabrication, which may prove especially useful for the deposition of enzyme at selected sites on microelectrode array microprobes of the kind used for neuroscience research in vivo.

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“…Some of the excellent features of chitosan are non-toxicity, homeostasis, antibacterial, biocompatibility and biodegradability [8,9]. These advantageous qualities make chitosan suitable for a variety of biomedical purposes such as wound healing [10][11][12][13], tissue engineering scaffolds [14][15][16][17], drug delivery systems [18] and biosensors [19]. Despite its exceptional properties, chitosan has been of limited biomaterial use in biomedical engineering due to its high molecular weight making it insoluble in water at a neutral or basic pH and in other organic solvents.…”

Section: Introductionmentioning

confidence: 99%

UV irradiation- $$\hbox {H}_{2} \hbox {O}_{2}$$ H 2 O 2 system as an effective combined depolymerization technique to produce

et al. 2018

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UV irradiation hydrogen peroxide (H 2 O 2 ) system is used as an effective, easy and low-cost combined depolymerization technique to produce oligosaccharides from chitosan. UV-Vis spectroscopic studies explained that with increasing treatment time, the absorption of the depolymerized chitosan solution has increased, indicating the increase in the carbonyl and amino groups in their structure. Fourier transform infrared spectroscopy and nuclear magnetic resonance (1H NMR) analysis showed that the 1,4-β-d-glucoside linkages of chitosan are degraded without important changes in chemical structure of decomposed samples. X-ray diffraction patterns verified the polymerization of chitosan to produce oligomers, changing in structure from crystalline to amorphous. Viscosity-average molecular weight measurements of fragmented chitosan samples and MarkHouwink equation are used to demonstrate the efficiency of this depolymerization method. Finally, the obtained results ascertained that this combined method could produce water soluble chitosan with significant efficiency and no essential change in its chemical structure.

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Amperometric glucose biosensor based on glucose oxidase immobilized over chitosan nanoparticles from gladius of Uroteuthis duvauceli

Cited by 68 publications

References 50 publications

Novel amperometric glucose biosensor based on MXene nanocomposite

Novel amperometric glucose biosensor based on MXene nanocomposite

Enzyme Deposition by Polydimethylsiloxane Stamping for Biosensor Fabrication

UV irradiation- $$\hbox {H}_{2} \hbox {O}_{2}$$ H 2 O 2 system as an effective combined depolymerization technique to produce

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