Amperometric enzyme electrode for aerobic glucose monitoring prepared by glucose oxidase immobilized in poly(vinylferrocenium)

Gülce, Handan; Özyörük, Haluk; Çelebi, Süleyman; Yıldız, Attila

doi:10.1016/0022-0728(95)04013-e

Cited by 60 publications

(26 citation statements)

References 36 publications

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“…Furthermore, the amount of the enzyme immobilized within the polymer could then be determined using the same absorption peak after desorbing the enzyme in a solution having a pH value less than the isoelectric point of the corresponding enzyme. 3 The activity of the enzyme electrode was determined with a jacketed electrochemical cell, which kept the solution at the desired temperature. Oxygen was introduced into the solution in this cell at a constant flow rate to obtain an oxygen-saturated solution.…”

Section: (Received May 25 2001; Accepted October 22 2001)mentioning

confidence: 99%

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A Novel Two-Enzyme Amperometric Electrode for Lactose Determination

Gülce

Yıldız

2002

ANAL. SCI.

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Section: (Received May 25 2001; Accepted October 22 2001)mentioning

confidence: 99%

“…1,2 The use of a redox polymer, polyvinylferrocenium perchlorate (PVF + ClO4 -), as an immobilization matrix allowed the development of novel amperometric sensors for glucose, 3 galactose 4 and sucrose. 5 A single enzyme glucose oxidase or galactose oxidase was immobilized in the first two enzyme sensors.…”

Section: Introductionmentioning

confidence: 99%

A Novel Two-Enzyme Amperometric Electrode for Lactose Determination

Gülce

Yıldız

2002

ANAL. SCI.

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“…55,56,58,69 The redox polymer, polyvinylferrocenium perchlorate, behaved like a modified surface through which an electron transfer between a substrate and a reactant took place. 2,55 The polyvinylferrocenium-coated electrode was simple, fast, sensitive, and stable and had a constant and maximum response time at the temperature of physiological fluids.…”

Section: ·3 Coated Electrodes For Polyvinylferroceniummentioning

confidence: 99%

“…56 A new amperometric enzyme electrode in polyvinylferrocenium perchlorate was proposed for the development of the alcohol biosensor. 58 The sensitivity of the enzyme electrode was based on four types of alcohol: methanol, ethanol, 1-butanol, and benzyl alcohol.…”

Section: ·3 Coated Electrodes For Polyvinylferroceniummentioning

confidence: 99%

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Recent Developments, Characteristics, and Potential Applications of Electrochemical Biosensors

2004

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The objective of this study is to analyze the technical importance, performance, techniques, advantages, and disadvantages of the biosensors in general and of the electrochemical biosensors in particular. A product of reaction diffuses to the transducer in the first generation biosensors (based on Clark biosensors). The mediated biosensors or second generation biosensors use specific mediators between the reaction and the transducer to improve sensitivity. The second generation biosensors involve two steps: first, there is a redox reaction between enzyme and substrate that is reoxidized by the mediator, and eventually the mediator is oxidized by the electrode. No normal product or mediator diffusion is directly involved in the third generation biosensors, direct biosensors. Based on the type of transducer, current biosensors are divided into optical, mass, thermal, and electrochemical sensors. They are used in medical diagnostics, food quality controls, environmental monitoring, and other applications. These biosensors are also grouped under two broad categories of sensors: direct and indirect detection systems. Moreover, these systems could be further grouped into continuous or batch operation. Therefore, amperometric biosensors and their current applications are focused on more in detail since they are the most commonly used biosensors in monitoring and diagnosing tests in clinical analysis. Problems related to the commercialization of medical, environmental, and industrial biosensors as well as their performance characteristics, their competitiveness in comparison to the conventional analytical tools, and their costs determine the future development of these biosensors.

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Amperometric Glucose Enzyme Sensor Prepared by Immobilizing Glucose Oxidase on CuPtCl6 Chemically Modified Electrode

Pei

1999

Electroanalysis

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A simple, sensitive, stable and low-cost glucose sensor with a fast response that operates under aerobic solution conditions is described. The electrode consisted of a CuPtCl 6 modi®ed ®lm deposited on the surface of the glassy carbon electrode onto which the glucose oxidase was immobilized via cross-linking of glutaraldehyde in the presence of bovine serum albumin (BSA). The electropolymerized CuPtCl 6 ®lm was prepared by continuously cyclically scanning between 700 mV and À800 mV (vs. SCE) in a solution containing CuCl 2 and K 2 PtCl 6 . The preparation and the performance of the enzyme electrode have been discussed. The effects of enzyme loading, pH, and the temperature have been studied. The experiments on interference and speci®city have also been demonstrated. The enzyme electrode exhibits an excellent biocatalytic activity and stability. The enzyme electrode provides a linear response to glucose over a concentration range of 2610 À6 M to 1610 À3 M with a detection limit of 1610 À6 M.

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Amperometric enzyme electrode for aerobic glucose monitoring prepared by glucose oxidase immobilized in poly(vinylferrocenium)

Cited by 60 publications

References 36 publications

A Novel Two-Enzyme Amperometric Electrode for Lactose Determination

A Novel Two-Enzyme Amperometric Electrode for Lactose Determination

Recent Developments, Characteristics, and Potential Applications of Electrochemical Biosensors

Amperometric Glucose Enzyme Sensor Prepared by Immobilizing Glucose Oxidase on CuPtCl6 Chemically Modified Electrode

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