Amperometric determination of heavy metal using an HRP inhibition biosensor based on ITO nanoparticles-ruthenium (III) hexamine trichloride composite: Central composite design optimization

Dalkıran, Berna

doi:10.1016/j.bioelechem.2020.107569

Cited by 22 publications

(10 citation statements)

References 89 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…134−137 Of particular interest is the ability of many indirect mode enzymatic biosensors to detect heavy metals ions such as Hg 2+ , Ni 2+ , Pb 2+ , and Cd 2+ at concentrations below WHO guideline values for drinking water. 136,138 In addition, many researchers are now taking advantage of nanomaterials in the fabrication of enzyme-based biosensors that due to their enhanced conductivity help to improve the signal-to-noise ratio and signal transduction. 49 However, enzyme immobilization remains a challenge, which is crucial for the stability, shelf life, reproducibility, and repeatability of enzyme-based biosensors.…”

Section: Environmental Toxicant Detection Usingmentioning

confidence: 99%

“…Enzymatic biosensors have demonstrated the ability to detect various heavy metal ions, including Pb 2+ , Cu 2+ , Cd 2+ , Cr(VI), and Hg 2+ . − These indirect mode biosensors are based upon the inhibition of enzyme (urease, horseradish peroxidase, glucose oxidase, and catalase) activity in the presence toxic heavy metals. Their advantages include low LODs (up to nanomolar concentrations), high selectivity, repeatability, and in some cases storage at 4 °C from 14 to 30 days. − Of particular interest is the ability of many indirect mode enzymatic biosensors to detect heavy metals ions such as Hg 2+ , Ni 2+ , Pb 2+ , and Cd 2+ at concentrations below WHO guideline values for drinking water. , In addition, many researchers are now taking advantage of nanomaterials in the fabrication of enzyme-based biosensors that due to their enhanced conductivity help to improve the signal-to-noise ratio and signal transduction . However, enzyme immobilization remains a challenge, which is crucial for the stability, shelf life, reproducibility, and repeatability of enzyme-based biosensors …”

Section: Environmental Toxicant Detection Using Electrochemical Biose...mentioning

confidence: 99%

See 1 more Smart Citation

Electrochemical Biosensors for Detection of Pesticides and Heavy Metal Toxicants in Water: Recent Trends and Progress

Hara¹,

Singh

2021

ACS EST Water

127

View full text Add to dashboard Cite

The release of chemicals into water systems has resulted in pollution in many parts of the world, threatening human health and aquatic ecosystems. Sources of chemical discharge include industry, agricultural, wastewater treatment plants, and stormwater overflows. To combat water pollution, the European Union has introduced several directives that set concentration limits for chemicals in drinking water, surface water, and groundwater. To meet these limits, it is essential that rapid, reliable, and sensitive analytical detection systems be developed and put into use. This Review presents the progress made in the development of electrochemical biosensors for environmental toxicants (pesticides and heavy metals) over the past seven years (2014–2020). For those unfamiliar with this field of research, the concept of a biosensor is introduced followed by a critical evaluation of their performance in detecting the toxicants. Current challenges are discussed as well as potential avenues for future research, including the demands for enhanced analytical performance, improved biosensor stability and shelf life, and greater integration with microfluidic devices and wireless database technologies for remote environmental sensing applications. We believe that this Review will be beneficial and enhance awareness and appreciation of the role that electrochemical biosensors can play in protecting our environment and water resources.

show abstract

Section: Environmental Toxicant Detection Usingmentioning

confidence: 99%

Section: Environmental Toxicant Detection Using Electrochemical Biose...mentioning

confidence: 99%

Electrochemical Biosensors for Detection of Pesticides and Heavy Metal Toxicants in Water: Recent Trends and Progress

Hara¹,

Singh

2021

ACS EST Water

127

View full text Add to dashboard Cite

show abstract

“…Several enzymes, such as urease [347][348][349], horseradish peroxidase [343,350,351], glucose oxidase [287,352], invertase [353,354], and dehydrogenase [355][356][357] can be highly efficient in metal identification analyses, based either on the activation or on the inhibition of the specific enzyme. Heavy metals generally act as cofactors of metalloproteins to form an essential part of the structure that is required for enzyme activation [335,342].…”

Section: Heavy Metal Sensingmentioning

confidence: 99%

“…Enzymatic methods are based on the inhibition behavior that several metal ions can cause in a wide range of enzymes (such as peroxidases, dehydrogenases, ureases), which are explicitly inhibited by low concentrations of a particular metal ion [353,[359][360][361][362]. Nanoparticles can enhance electron transfer, increase conductivity, and offer high biocompatibility and catalytic efficiency, therefore composing inhibition biosensors with high sensitivity [350,362].…”

Section: Heavy Metal Sensingmentioning

confidence: 99%

Designing of Nanomaterials-Based Enzymatic Biosensors: Synthesis, Properties, and Applications

et al. 2021

View full text Add to dashboard Cite

Among the many biological entities employed in the development of biosensors, enzymes have attracted the most attention. Nanotechnology has been fostering excellent prospects in the development of enzymatic biosensors, since enzyme immobilization onto conductive nanostructures can improve characteristics that are crucial in biosensor transduction, such as surface-to-volume ratio, signal response, selectivity, sensitivity, conductivity, and biocatalytic activity, among others. These and other advantages of nanomaterial-based enzymatic biosensors are discussed in this work via the compilation of several reports on their applications in different industrial segments. To provide detailed insights into the state of the art of this technology, all the relevant concepts around the topic are discussed, including the properties of enzymes, the mechanisms involved in their immobilization, and the application of different enzyme-derived biosensors and nanomaterials. Finally, there is a discussion around the pressing challenges in this technology, which will be useful for guiding the development of future research in the area.

show abstract

“…Another reason to keep the number of enzymes at minimum for cascade reactions is the fact that some enzymes are inhibited by various compounds that could be present in the sample matrix. Thus, the peroxidase that is used to facilitate the electrochemical detection of H 2 O 2 is inhibited by compounds such as: glyphosate [ 71 ], heavy metals [ 72 ] or sulfide [ 73 ] to such an extent that it was used alone to make inhibition based biosensor for these compounds. Same potential inhibition should be taken into consideration also when using diaphorase for NA(D)PH electrochemistry purposes, this particular situation being even more complex since numerous enzymes are referred under the generic “diaphorase” name and thus the screening of the databases for potential inhibitors is even more complicated.…”

Section: The Innovative Use Of Enzyme Kinetic Particularities To Improve the Selectivitymentioning

confidence: 99%

Addressing the Selectivity of Enzyme Biosensors: Solutions and Perspectives

Bucur

Purcarea²,

Andreescu

et al. 2021

Sensors

View full text Add to dashboard Cite

Enzymatic biosensors enjoy commercial success and are the subject of continued research efforts to widen their range of practical application. For these biosensors to reach their full potential, their selectivity challenges need to be addressed by comprehensive, solid approaches. This review discusses the status of enzymatic biosensors in achieving accurate and selective measurements via direct biocatalytic and inhibition-based detection, with a focus on electrochemical enzyme biosensors. Examples of practical solutions for tackling the activity and selectivity problems and preventing interferences from co-existing electroactive compounds in the samples are provided such as the use of permselective membranes, sentinel sensors and coupled multi-enzyme systems. The effect of activators, inhibitors or enzymatic substrates are also addressed by coupled enzymatic reactions and multi-sensor arrays combined with data interpretation via chemometrics. In addition to these more traditional approaches, the review discusses some ingenious recent approaches, detailing also on possible solutions involving the use of nanomaterials to ensuring the biosensors’ selectivity. Overall, the examples presented illustrate the various tools available when developing enzyme biosensors for new applications and stress the necessity to more comprehensively investigate their selectivity and validate the biosensors versus standard analytical methods.

show abstract

Amperometric determination of heavy metal using an HRP inhibition biosensor based on ITO nanoparticles-ruthenium (III) hexamine trichloride composite: Central composite design optimization

Cited by 22 publications

References 89 publications

Electrochemical Biosensors for Detection of Pesticides and Heavy Metal Toxicants in Water: Recent Trends and Progress

Electrochemical Biosensors for Detection of Pesticides and Heavy Metal Toxicants in Water: Recent Trends and Progress

Designing of Nanomaterials-Based Enzymatic Biosensors: Synthesis, Properties, and Applications

Addressing the Selectivity of Enzyme Biosensors: Solutions and Perspectives

Contact Info

Product

Resources

About