Amperometric Detection of 3-(3,4-Dihydroxyphenyl)-l-alanine (l-Dopa) in Raw and Cooked Mucuna Bean Seeds Employing Micro-HPLC

Mwatseteza, Jonas; Torto, Nelson

doi:10.1365/s10337-007-0397-1

Cited by 7 publications

(4 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nowadays (Mwatseteza and Torto ), different works have reported other natural sources of L‐DOPA such as seeds of Mucuna pruriens , they stated that after cooking and processing, “black” and “white” varieties showed a decrease in the L‐DOPA content from 4.97 and 6.84% up to 0.26 and 0.67%, respectively.…”

Section: Resultsmentioning

confidence: 99%

Effect of Roasting and Boiling on the Content of Vicine, Convicine and L‐3,4‐dihydroxyphenylalanine in Vicia fabaL.

Cardador‐Martínez

Maya-Ocaña

Ortiz-Moreno

et al. 2012

Journal of Food Quality

View full text Add to dashboard Cite

The pyrimidine glycosides, vicine and convicine, are compounds of the genus Vicia, whereas their aglycone derivatives, divicine and isouramil, are responsible for favism occurrence, and L‐3,4‐dihydroxyphenylalanine (L‐DOPA) is a nonprotein amino acid precursor of dopamine in healthy neurons. The present work evaluated the effect of thermal treatment, which consisted of roasting and boiling, on the content of vicine, convicine and L‐DOPA in mature cotyledons of 10 varieties of Mexican Vicia faba L. and determined the concentration of glycosides and L‐DOPA by high‐performance liquid chromatography. The raw cotyledons showed 2.88–6.10, 0.63–1.68 and 0.28–0.44 vicine, convicine and L‐DOPA, respectively. The 12 and 40% and 30 and 61% decrease in vicine and convicine content, respectively, were observed to be the effect of roasting and boiling the samples. L‐DOPA was totally removed by both thermal processes. In all analyzed samples, vicine was the main compound; however, there were significant differences between varieties and thermal treatments. Practical Applications Vicine and convicine glycosides are strongly implicated in favism, a hemolytic anemia in humans, and in poor performance of laying hens. There are reports of decreased egg weight, increase in the fragility of the yolk and a number of blood stains, and decreased fertility and hatchability of eggs. The characterization of new varieties of faba bean in their content of potentially toxic glycosides allows selection of those with the lowest possible content of such compounds. Also, the application of heat treatments that reduce glycosides will allow the use of seeds or flour to develop harmless food and feed ingredients.

show abstract

Section: Resultsmentioning

confidence: 99%

Effect of Roasting and Boiling on the Content of Vicine, Convicine and L‐3,4‐dihydroxyphenylalanine in Vicia fabaL.

Cardador‐Martínez

Maya-Ocaña

Ortiz-Moreno

et al. 2012

Journal of Food Quality

View full text Add to dashboard Cite

show abstract

“…Ingestion of excessive amounts of Lev can lead to severe psychosis nausea, emesis, hypotension and dyskinesia. In vitro, Lev is a lethal toxin to the culture of neurons and a few animal studies have shown that chronic Lev may be toxic in vivo, too [34][35][36][37][38]. Several techniques have been developed in order to determine Lev levels in different sample matrices including: spectrophotometry [39][40][41], high-performance liquid chromatography [42][43][44], flow injection analysis [45], capillary zone electrophoresis with electrochemical detection [46].…”

Section: Introductionmentioning

confidence: 99%

A Selective Simultaneous Determination of Levodopa and Serotonin Using a Glassy Carbon Electrode Modified with Multiwalled Carbon Nanotube/Chitosan Composite

Babaei

Babazadeh

2011

Electroanalysis

View full text Add to dashboard Cite

A glassy carbon electrode (GCE) modified with carbon nanotube/chitosan (MWCNTs-CHT/GCE) was used for the sensitive voltammetric determination of levodopa (Lev) and serotonin (Ser). The measurements were carried out using cyclic voltammetry (CV), differential pulse voltammetry (DPV) and chronoamperometry (CA). Under the optimum conditions the electrode provides a linear response versus Lev and Ser concentrations in the range of 2.0-220.0 mM and 0.5-130.0 mM, respectively, using DPV. The modified electrode was satisfactorily used for determination of Lev and Ser in human serum and urine with satisfactory results.

show abstract

“…By using high performance liquid chromatography HPLC-UV, different analytical techniques have been used to identify L-Dopa in various plant materials (Baranowska & Płonka, 2015;Siddhuraju & Becker, 2001;Goyoaga et al, 2008;Renna et al, 2020;Etemadi et al 2018;Randhir et al, 2002;Yang et al, 2001;Dhanani et al, 2015;Bulduk & Topal, 2020;Polanowska et al, 2019;Kasture et al, 2014;Rathod & Patel, 2014;Patil et al, 2015;Singh et al 2010). Liquid chromatography-mass spectrometry/mass spectrometry LC MS/MS (Pavón-Pérez et al, 2019;Abdel-Sattar et al, 2021;Varga & Varga, 2014), UV spectrophotometry (Vadivel & Biesalski, 2012;Rahmani-Nezhad et al, 2018), High-performance thin-layer chromatography (HPTLC) (Aware et al, 2017), Capillary electrophoresis with ultra-violet detection CE-UV (Chen et al, 2005), Electrochemical methods (Kalachar et al, 2011;Mwatseteza & Torto, 2007;Arvand et al, 2016;Rwei et al, 2018), and Nuclear Magnetic Resonance NMR (Fernandez-Pastor et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of Analytical Methods for Quantification of L-Dopa; Application to Plant Materials

Akbel¹,

Bulduk²

2023

IJIAAR

View full text Add to dashboard Cite

An essential neurotransmitter used to treat nervous system dysfunction like Parkinson's disease is L-dopa. The amount of L-dopa in plant materials has been measured in this study using chromatographic and spectrophotometric methods that are user-friendly, straightforward, quick, and affordable. Utilizing conventional instrumental parameters, high-performance liquid chromatography and spectrophotometry methods were established. In accordance with ICH guidelines, these analytical methods were validated for linearity, accuracy, precision, and robustness. The chromatographic procedure made use of an Agilent Extend C18 (250×4.6 mm, 5 µm) column. 0.1% trifloroacetic acid solution and acetonitrile (92/8, v/v) were used as mobile phase. It was run in isocrotic mode and the flow rate was 1 mL min-1. The retention time of L-dopa was determined as 3.85 minutes. L-dopa was identified using the spectrophotometric method, which involved measuring the solutions' absorbance at a wavelength of 280 nm. In spectrophotometric analysis, ultra-pure water as a solvent gave sufficient molar absorptivity at a λmax of 280 nm. The results showed that spectrophotometric and chromatographic methods were linear, accurate, precise, robust, and the percent recovery was within standard limits. No statistically significant distinction existed between the methods within the 95% confidence interval (p<0.05). The developed methods can be used to quantify L-dopa in plant materials for routine analysis and have been found to be very efficient.

show abstract

Amperometric Detection of 3-(3,4-Dihydroxyphenyl)-l-alanine (l-Dopa) in Raw and Cooked Mucuna Bean Seeds Employing Micro-HPLC

Cited by 7 publications

References 14 publications

Effect of Roasting and Boiling on the Content of Vicine, Convicine and L‐3,4‐dihydroxyphenylalanine in Vicia fabaL.

Effect of Roasting and Boiling on the Content of Vicine, Convicine and L‐3,4‐dihydroxyphenylalanine in Vicia fabaL.

A Selective Simultaneous Determination of Levodopa and Serotonin Using a Glassy Carbon Electrode Modified with Multiwalled Carbon Nanotube/Chitosan Composite

Development and Validation of Analytical Methods for Quantification of L-Dopa; Application to Plant Materials

Contact Info

Product

Resources

About