Amperometric detection in microchip electrophoresis devices: Effect of electrode material and alignment on analytical performance

Fischer, David J.; Hulvey, Matthew K.; Regel, A. R.; Lunte, Susan M.

doi:10.1002/elps.200900317

Cited by 50 publications

(43 citation statements)

References 45 publications

(45 reference statements)

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“…An additional reservoir, placed 200 µm away from the waste reservoir, and filled with a PDMS "sponge" [40] was used as a decoupler. The decoupler, similar in design to the one reported by Lunte et al [41], was selected to minimize the formation of bubbles in the separation channel and decrease the noise of the detection step [42][43][44].…”

Section: Instrumentationmentioning

confidence: 99%

Fast and versatile fabrication of PMMA microchip electrophoretic devices by laser engraving

Gabriel,

Coltro,

Garcia

2014

Electrophoresis

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This paper describes the effects of different modes and engraving parameters on the dimensions of microfluidic structures produced in PMMA using laser engraving. The engraving modes included raster and vector while the explored engraving parameters included power, speed, frequency, resolution, line-width and number of passes. Under the optimum conditions, the technique was applied to produce channels suitable for CE separations. Taking advantage of the possibility to cut-through the substrates, the laser was also used to define solution reservoirs (buffer, sample, and waste) and a PDMS-based decoupler. The final device was used to perform the analysis of a model mixture of phenolic compounds within 200 s with baseline resolution.

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Section: Instrumentationmentioning

confidence: 99%

Fast and versatile fabrication of PMMA microchip electrophoretic devices by laser engraving

Gabriel,

Coltro,

Garcia

2014

Electrophoresis

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“…However, this article presents electrode materials for general electroactive analytes. Electrode material has a significant impact on the performance of the MCE, and many researchers have focused on this area, in order to achieve more stable and sensitive microchips with higher resolution [4][5][6]. Working electrodes, at which the redox reactions occur, generate the interested signal or data by which ECD of the analytes takes place.…”

Section: Introductionmentioning

confidence: 99%

“…One of the factors is the fabrication materials. When fabricating the MCE, variety of materials can be used for electrodes fabrication, substrates and microchannels fabrication [4].…”

Section: Introductionmentioning

confidence: 99%

Design of DNA biosensors and amperometric microchips featuring copper electrodes

Ghanim

Hasan

Najimudin

et al. 2012

Proceedings of 2012 IEEE-EMBS International Conference on Biomedical and Health Informatics

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Electrochemical detection (ECD) is one of the best means for producing DNA biosensors and disposable microchips, oriented for electroactive analyte's separation and detection applications like biomedical and in vitro diagnostic. Electrode material is one of the major factors that influence the performance and development of biosensors and ECD microchips. Traditionally, the ECD sensing has been based on platinum electrode which is costly though it offers good current sensitivity. In this study a copper electrode has been investigated as an alternative sensing strategy. Preliminary results indicate that the copper electrodes are also capable of performing both detection and separation, yielding a significant limit of detection. On average the copper electrodes are capable of detecting the redox currents which are consistently higher 10 IlA compared to 30 IlA for platinum electrodes using DNAanalytes. This work will pave the way in designing a more affordable and disposable microchips.

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“…For these reasons, ED has been an important alternative to LIF detection. Amperometry [35][36][37], conductimetry [38][39][40][41], and potentiometry [42] are the three electrochemical methods employed in combination with MCE.…”

Section: Introductionmentioning

confidence: 99%

New analytical portable instrument for microchip electrophoresis with electrochemical detection

Fernández-la-Villa¹,

Pozo-Ayuso²,

Castaño‐Álvarez³

2010

Electrophoresis

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A new portable instrument that includes a high voltage power supply, a bipotentiostat, and a chip holder has been especially developed for using microchips electrophoresis with electrochemical detection. The main unit of the instrument has dimensions of 150 x 165 x 70 mm (wxdxh) and consists of a four-outputs high voltage power supply with a maximum voltage of +/-3 KV and an acquisition system with two channels for dual amperometric (DC or pulsed amperometric detection) detection. Electrochemical detection has been selected as signal transduction method because it is relatively easily implemented, since nonoptical elements are required. The system uses a lithium-ion polymer battery and it is controlled from a desktop or laptop PC with a graphical user interface based on LabVIEW connected by serial RS232 or Bluetooth. The last part of the system consists of a reusable chip holder for housing the microchips, which contain all the electrical connections and reservoirs for making the work with microchips easy. The performance of the new instrument has been evaluated and compared with other commercially available apparatus using single- and dual-channel pyrex microchips for the separation of the neurotransmitters dopamine, epinephrine, and 3,4-dihydroxy-L-phenyl-alanine. The reduction of the size of the instrument has not affected the good performance of the separation and detection using microchips electrophoresis with electrochemical detection. Moreover, the new portable instrument paves the way for in situ analysis making the use of microchips electrophoresis easier.

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Amperometric detection in microchip electrophoresis devices: Effect of electrode material and alignment on analytical performance

Cited by 50 publications

References 45 publications

Fast and versatile fabrication of PMMA microchip electrophoretic devices by laser engraving

Fast and versatile fabrication of PMMA microchip electrophoretic devices by laser engraving

Design of DNA biosensors and amperometric microchips featuring copper electrodes

New analytical portable instrument for microchip electrophoresis with electrochemical detection

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