Amperometric cholesterol biosensor based on cholesterol oxidase and Pt-Au/ MWNTs modified glassy carbon electrode

Haritha, V.S.; Kumar, S. R. Sarath; Rakhi, R.B.

doi:10.1016/j.matpr.2021.03.128

Cited by 15 publications

(9 citation statements)

References 22 publications

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“…[ 20 ] The amperometric sensor can detect a wide variety of biomarkers, including glucose, lactate, and tiny gas molecules (e.g., alcohol and oxygen). [ 21 ]…”

Section: Electrochemical Sensors For Detecting Analytes In Various Bi...mentioning

confidence: 99%

“…[20] The amperometric sensor can detect a wide variety of biomarkers, including glucose, lactate, and tiny gas molecules (e.g., alcohol and oxygen). [21] Voltammetric Sensors: A voltammetric sensor measures a produced current between the WE and CE upon sweeping a potential between the WE and RE, where the measured current is consequence of the electrochemical reaction (e.g., oxidation, reduction) between the electroactive recognition elements and the target analyte on the surface of WE. Current responses are often recorded as a peak, which corresponds to the electroactive species concentration.…”

Section: Working Principlesmentioning

confidence: 99%

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Advances in Electrochemical Sensors for Detecting Analytes in Biofluids

Lee

Kim

Soltis

et al. 2023

Advanced Sensor Research

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More than half of all Americans suffer from chronic diseases, the leading causes of death and disability. However, prompt treatment of chronic diseases can lead to better patient outcomes and a reduced burden on the healthcare system. This highlights the urgent need for electrochemical (EC) sensors that provide non‐invasive, real‐time monitoring of disease‐indicating biomarkers. Due to their high sensitivity, high selectivity, and cost‐effectiveness, EC biosensors have recently shown tremendous promise for individualized health monitoring. This review explains the working principles of EC biosensors. It summarizes the recent advances and improvements of EC biosensors for detecting biomarkers in different biofluids, including tears, saliva, breath, urine, and sweat. Through a comprehensive overview of EC biosensor technologies, this article is expected to aid the development of flexible and wearable EC biosensing systems that have the potential to provide continuous, long‐term health monitoring for both clinical and at‐home use.

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“…[ 20 ] The amperometric sensor can detect a wide variety of biomarkers, including glucose, lactate, and tiny gas molecules (e.g., alcohol and oxygen). [ 21 ]…”

Section: Electrochemical Sensors For Detecting Analytes In Various Bi...mentioning

confidence: 99%

Section: Working Principlesmentioning

confidence: 99%

Advances in Electrochemical Sensors for Detecting Analytes in Biofluids

Lee

Kim

Soltis

et al. 2023

Advanced Sensor Research

View full text Add to dashboard Cite

show abstract

“…Due to the synergistic effects of the AuNP and TiS 2 NS for increasing the redox signals produced from the enzymatic reaction between uricase and uric acid, the developed nanobiosensor successfully detected uric acid prepared with interfering molecules, such as ascorbic acid, urea, and glucose, and detected uric acid dissolved in commercial human serum [ 80 ]. In another study, gold (Au) and platinum (Pt)-decorated CNTs were proposed as the nanocomposite for the immobilization of cholesterol oxidase (COx) to develop an enzymatic electrochemical cholesterol nanobiosensor [ 81 ]. In addition to the metallic nanocomposite, other materials, such as organic materials, are also being incorporated with metallic nanomaterials to develop enzymatic nanobiosensors.…”

Section: Enzymatic Electrochemical Nanobiosensorsmentioning

confidence: 99%

Enzymatic Electrochemical/Fluorescent Nanobiosensor for Detection of Small Chemicals

Choi

Yoon

2023

Biosensors

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The detection of small molecules has attracted enormous interest in various fields, including the chemical, biological, and healthcare fields. In order to achieve such detection with high accuracy, up to now, various types of biosensors have been developed. Among those biosensors, enzymatic biosensors have shown excellent sensing performances via their highly specific enzymatic reactions with small chemical molecules. As techniques used to implement the sensing function of such enzymatic biosensors, electrochemical and fluorescence techniques have been mostly used for the detection of small molecules because of their advantages. In addition, through the incorporation of nanotechnologies, the detection property of each technique-based enzymatic nanobiosensors can be improved to measure harmful or important small molecules accurately. This review provides interdisciplinary information related to developing enzymatic nanobiosensors for small molecule detection, such as widely used enzymes, target small molecules, and electrochemical/fluorescence techniques. We expect that this review will provide a broad perspective and well-organized roadmap to develop novel electrochemical and fluorescent enzymatic nanobiosensors.

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“…Biosensor applications include the areas of medicine (Parthasarathy & Vivekanandan, 2019;Uno, 2019), environment (Kaffash, Rostami, Zare, 2019), public safety and food safety (Semenova et al, 2018). For medical applications, there are: clinics (Haritha, Kumar, Rakhi, 2022) Modeling, due to its nature, proves to be extremely useful for the creation and manipulation of specific systems, given the use of the general model created and using the desired data, with the physical and mathematical model of the same, we can assign specific values in order to study and evaluate the behavior of the system in different scenarios (Kaffash, Rostami, Zare, 2019).…”

Section: Introductionmentioning

confidence: 99%

Ciência e tecnologia: eixos do desenvolvimento

2022

Ciência E Tecnologia: Eixos Do Desenvolvimento

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Biosensors are a class of sensors aimed at carrying out measurements involving organic substances. These sensors have many advantages for use, ranging from miniaturization to portability of the equipment, thus enabling their use in an easy way and in a very wide range of locations. This work has the focus of evaluating the functioning of a monolayer enzymatic amperometric biosensor through a process of mathematical modeling of its working principle from Partial Differential Equations (PDEs) and the boundary/initial conditions that govern and characterize this type of equipment, the systems of equations created were solved based on the Finite Difference Method (FDM) techniques and with the aid of a computer code to provide the solution for the evaluated system. Finally, tests were simulated to evaluate the impacts that each factor plays on the system response, sensitivity tests were performed for each of its parameters, by means of a monolayer enzymatic amperometric biosensor. With the results, it was possible to observe the great impacts that factors such as the maximum enzymatic rate (VMAX), the enzymatic layer thickness (d) and the initial concentration of the substrate (S0) have on the system response, which are considered crucial in a project of biosensors.

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Amperometric cholesterol biosensor based on cholesterol oxidase and Pt-Au/ MWNTs modified glassy carbon electrode

Cited by 15 publications

References 22 publications

Advances in Electrochemical Sensors for Detecting Analytes in Biofluids

Advances in Electrochemical Sensors for Detecting Analytes in Biofluids

Enzymatic Electrochemical/Fluorescent Nanobiosensor for Detection of Small Chemicals

Ciência e tecnologia: eixos do desenvolvimento

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