Amperometric catechol biosensor based on laccase immobilized on nitrogen-doped ordered mesoporous carbon (N-OMC)/PVA matrix

Abstract

A functionalized nitrogen-containing ordered mesoporous carbon (N-OMC), which shows good electrical properties, was synthesized by the carbonization of polyaniline inside a SBA-15 mesoporous silica template. Based on this, through entrapping laccase onto the N-OMC/polyvinyl alcohol (PVA) film a facilely fabricated amperometric biosensor was developed. Laccase from Trametes versicolor was assembled on a composite film of a N-OMC/PVA modified Au electrode and the electrochemical behavior was investigated. The results indicated that the N-OMC modified electrode exhibits electrical properties towards catechol. The optimum experimental conditions of a biosensor for the detection of catechol were studied in detail. Under the optimal conditions, the sensitivity of the biosensor was 0.29 A*M−1 with a detection limit of 0.31 μM and a linear detection range from 0.39 μM to 8.98 μM for catechol. The calibration curve followed the Michaelis–Menten kinetics and the apparent Michaelis–Menten was 6.28 μM. This work demonstrated that the N-OMC/PVA composite provides a suitable support for laccase immobilization and the construction of a biosensor.

A novel Laccase Biosensor based on Laccase immobilized Graphene-Cellulose Microfiber Composite modified Screen-Printed Carbon Electrode for Sensitive Determination of Catechol

“…In order to evaluate the novelty and superiority of the biosensor, we made the comparison table for analytical performance of the as-prepared biosensor with previously reported laccase biosensors for CC and the comparative results are shown in Table 1. The comparative results clearly show that the as-prepared laccase biosensor exhibited a lower LOD (85 nM) towards CC than previously reported CC biosensors based on laccase immobilized on reduced graphene oxide supported palladium–copper alloyed nanocages42, nitrogen-doped ordered mesoporous/PVA matrix43, copper-containing ordered mesoporous carbon/chitosan matrix44, electrospun copper/carbon composite nanofibers45, 1-aminopyrene functionalized reduced graphene oxide46, carbon nanotubes–chitosan composite47, polyaniline48, multi-walled carbon nanotubes49 and ZnO sol-gel/chitosan modified electrodes50. However, the LOD of the as-fabricated CC biosensor is higher than the LOD (76 nM) of previously reported CC biosensor based on laccase immobilized reduced graphene oxide–glycol chitosan nanohybrid modified electrode, yet the sensitivity and linear response range of our biosensor is more comparable for the determination of CC.…”

Polyphenol oxidase (PPO) based biosensors for detection of phenolic compounds: A Review

“…Laccase shows a 4-electron reduction catalytic activity in which oxygen is reduced to water subsequently laccase oxidizes lot of phenolic substrate which makes laccase a potential candidate for fabrication in biosensors [90].…”

“…Laccase based amperometric biosensors are important class of biosensors in which current is produced as a result of enzymatic reaction and this change acts as a signal which is measured by physical techniques [1,72,90]. There is a linear relationship between observed current and amount of reactive species present.…”

“…There is a linear relationship between observed current and amount of reactive species present. Mostly platinum, gold or carbon based electrodes are constructed [84,90,91].…”

Synthesis and characterizations of nanoporous carbon derived from Lapsi (Choerospondias axillaris) seed: Effect of carbonization conditions

“…Porous materials have been significantly developed because of their fundamental properties and potential applications in many research areas including adsorption, storage, separation, and sensing [1][2][3][4][5][6]. Porous materials have been classified according to their pore diameters; micropores are those with diameters less than 2 nm, mesopores range from 2 nm to 50 nm, and macropores are larger than 50 nm in diameter.…”