Amperometric biosensor sensitive to glucose and lactose based on co-immobilization of ferrocene, glucose oxidase, β-galactosidase and mutarotase in β-cyclodextrin polymer

Liu, Haiying; Li, Haihong; Ying, Tailin; Sun, Kang; Qin, Yiqin; Qi, Dongtao

doi:10.1016/s0003-2670(97)00576-x

Cited by 79 publications

(45 citation statements)

References 23 publications

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“…All the parameters characteristic of our biosensor are reported in Table 1 together with the ones of other biosensors reported in the literature for the measurements of lactose and glucose. As can be seen, other biosensors [25][26][27][28] are characterized by a linear range larger than the one obtained by our biosensor, but its sensitivity is much higher than the others reported in the literature [25][26][27][28]. As far as LOD is concerned, it reaches very significant values for both the investigated saccharides.…”

Section: Resultscontrasting

confidence: 57%

“…The ferrocene was entrapped in the -cyclodextrin polymer and glucose oxidase, -galactosidase, and mutarotase were cross-linked with the -cyclodextrin polymer. In this case, cyclic voltammetry and amperometric measurements were employed in order to show the efficacy of electron transfer between immobilized glucose oxidase and a glassy carbon electrode via ferrocene included in the cavities of thecyclodextrin polymer [27]. More recently, Soldatkin et al [28] reported the development of an array of biosensors for the simultaneous determination of four carbohydrates in solution.…”

Section: Introductionmentioning

confidence: 99%

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Determination of Different Saccharides Concentration by Means of a Multienzymes Amperometric Biosensor

Portaccio

Lepore

2017

Journal of Sensors

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A three-electrode amperometric biosensor for the detection of two different saccharides (lactose and glucose) in aqueous solutions is described. On the graphite working electrode, the glucose oxidase (GOD) and -galactosidase ( -gal) were coimmobilized by means of covalent bonding. The response of the biosensor as a function of the relative concentration of the two immobilized enzymes was investigated and the best working conditions were identified by measuring the sensitivity and the linear range response. In particular, our best lactose biosensor shows a linear range up to 0.010 mM and a limit of detection (LOD) and a sensitivity equal to 0.001 mM and 850 ± 81 A/mM, respectively. For glucose, the values of the above-mentioned parameters are equal to 0.015 mM for the linear range, 0.001 mM for LOD, and 505 ± 55 A/mM for the sensitivity. The working parameters of our biosensors are significant in comparison with other biosensors developed for concentration determination of the two saccharides investigated in the present work. In particular, low (LOD) and high sensitivities are obtained for lactose and glucose. To challenge our biosensor with real samples, it was tested using fruit juices, skim milk, and whey.

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Section: Resultscontrasting

confidence: 57%

Section: Introductionmentioning

confidence: 99%

Determination of Different Saccharides Concentration by Means of a Multienzymes Amperometric Biosensor

Portaccio

Lepore

2017

Journal of Sensors

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“…Therefore, the effective electron transfer is obtained. If the DMAMF PPF was assumed to be monomer DMAMF with a density of 1.23 g/cm 3 , the concentration of ferrocene sites would be 5.1 × 10 -3 mol/cm 3 . Therefore, the surface coverage of 20 nm thickness film was 1.0 × 10 -10 mol/cm 2 .…”

Section: Characterization Of Dimethylaminomethylferrocene Plasma Polymentioning

confidence: 99%

“…A ferrocene is a typical mediator for enzyme biosensors. [1][2][3][4][5][6][7][8][9][10][11][12] Usually, the enzyme and mediators are embedded or immobilized in/onto a cast polymer on an electrode surface. The integrated design for sensor device between enzyme and redox site strongly influences the sensor characteristics.…”

Section: Introductionmentioning

confidence: 99%

Amperometric Glucose Biosensor Based on Mediated Electron Transfer between Immobilized Glucose Oxidase and Plasma-polymerized Thin Film of Dimethylaminomethylferrocene on Sputtered Gold Electrode

Kase

Muguruma

2004

ANAL. SCI.

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We propose an electron transfer-mediated amperometric enzyme biosensor based on plasma-polymerized thin film of dimethylaminomethylferrocene (DMAMF) on a sputtered gold electrode. The DMAMF plasma-polymerized film is deposited directly onto the surface of the electrode under dry conditions. The resulting thin film not only has redox sites but also is extremely thin (∼20 nm), adheres well onto the substrate (electrode), has a flat surface and a highly-crosslinked network structure, and is hydrophilic in nature. Glucose oxidase is densely immobilized onto the surface of DMAMF plasma-polymerized film on the gold electrode. From the electrochemical measurement, the biosensor can cover the wide range of glucose concentration (1.3 -81 mM) at +350 mV of applied potential. The current response of the glucose biosensor was decreased by less than 5% in an aerobic solution as compared to that in an anaerobic solution. These show that the DMAMF plasma-polymerized films play a role as the electron transfer mediators between the reaction center of enzyme and the electrode.

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“…= 0.6-0.8 V) interfering substances such as ascorbic acid and uric acid, which are commonly present in biological fluids are also oxidized, leading to nonspecific signals [3]. Several artificial redox mediators have been investigated as electron acceptors to solve these problems [4][5][6][7][8][9][10][11][12][13]. Additionally, the sensor electrodes have been modified to enhance the performance of amperometric glucose biosensors [14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Functionalised zinc oxide nanotube arrays as electrochemical sensors for the selective determination of glucose

et al. 2011

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Amperometric biosensor sensitive to glucose and lactose based on co-immobilization of ferrocene, glucose oxidase, β-galactosidase and mutarotase in β-cyclodextrin polymer

Cited by 79 publications

References 23 publications

Determination of Different Saccharides Concentration by Means of a Multienzymes Amperometric Biosensor

Determination of Different Saccharides Concentration by Means of a Multienzymes Amperometric Biosensor

Amperometric Glucose Biosensor Based on Mediated Electron Transfer between Immobilized Glucose Oxidase and Plasma-polymerized Thin Film of Dimethylaminomethylferrocene on Sputtered Gold Electrode

Functionalised zinc oxide nanotube arrays as electrochemical sensors for the selective determination of glucose

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