Amperometric Biosensor for Glutamate Using Prussian Blue-Based “Artificial Peroxidase” as a Transducer for Hydrogen Peroxide

Karyakin, Arkady A.; Karyakina, Elena E.; Gorton, Lo

doi:10.1021/ac990801o

Cited by 413 publications

(281 citation statements)

References 26 publications

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“…The inorganic nature of PB and its low cost makes it more suitable than HRP in assembling modified biosensors. PB modified glassy carbon (Itaya et al, 1984;Karyakin et al, 1996Karyakin et al, , 1998, graphite (Chi and Dong, 1995;Jaffari and Turner, 1997;Deng et al, 1998), carbon paste (Garjonyte and Malinauskas, 1998) and platinum (Itaya et al, 1982) electrodes have been studied, leading to the construction of glucose (Karyakin et al, 1995;Garjonyte and Malinauskas, 2000), lactate (Garjonyte et al, 2001), glutamate (Karyakin et al, 2000), aminoacid (Chi and Dong, 1995) and alcohol (Karyakin et al, 1996) biosensors. Recently, a review on the analytical applications of PB has appeared in literature (Karyakin, 2001).…”

Section: Introductionmentioning

confidence: 99%

Prussian Blue based screen printed biosensors with improved characteristics of long-term lifetime and pH stability

Ricci

Amine²,

Palleschi

et al. 2003

Biosensors and Bioelectronics

328

152

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The promising advantages of Prussian Blue (PB) as catalyst and of the thick film screen printing technology have been combined to assemble sensors with improved characteristics for the amperometric determination of H 2 O 2 . PB-modified screen printed electrodes were applied to detect H 2 O 2 at an applied potential of (/0.05 V versus the internal screen printed Ag pseudoreference electrode, showing a detection limit of 10 (7 mol l (1 , a linearity range from 10 (7 to 5 )/10 (5 mol l (1 , a sensitivity of 234 mA mmol l (1 cm (2 , and a high selectivity. Improved stability at alkaline pH values was also observed, which made possible their use with enzymes having an optimum basic pH. Then, the immobilisation of a single enzyme (glucose oxidase (GOD) or choline oxidase (ChOX)) or of two enzymes, acetylcholinesterase (AchE) coimmobilised with ChOX, has been performed on the surface of PB modified screen-printed electrodes (SPEs) using glutaraldehyde and Nafion † . ChOX has been selected as an example of enzyme working at alkaline pH. The choline biosensors showed a detection limit of 5 )/10 (7 mol l (1 , a wide linearity range (5 )/10 (7 Á/ 10 (4 mol l (1 ), a high selectivity and a remarkable long term stability of 9 months at 4 8C, and at least 4 weeks at room temperature. Similar analytical characteristics and stability were observed with the acetylcholine biosensors. #

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Section: Introductionmentioning

confidence: 99%

Prussian Blue based screen printed biosensors with improved characteristics of long-term lifetime and pH stability

Ricci

Amine²,

Palleschi

et al. 2003

Biosensors and Bioelectronics

328

152

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“…So far, much efforts have been devoted to develop detection methods of L-glutamate, such as high performance liquid chromatography (HPLC) [20], capillary electrophoresis [21,22], optical methods [23][24][25] and electrochemical analyses [26,27]. Among these approaches, electrochemical methods, especially electrochemical biosensors, have attracted much attention due to their high sensitivity, rapid response and easy operation [28][29][30][31]. However, some electrochemical biosensors had poor selectivity [32,33].…”

Section: Introductionmentioning

confidence: 99%

Amperometric l-glutamate biosensor based on bacterial cell-surface displayed glutamate dehydrogenase

Liang

Zhang

Lang

et al. 2015

Analytica Chimica Acta

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“…[16][17][18][19] To realize these applications, electrodes immobilized with high amounts of active components with good stability are usually required. The deposition of PB on various substrates through electrochemical and photochemical means have been reported.…”

Section: Introductionmentioning

confidence: 99%

Layer-by-layer Assembly of Prussian Blue and Carbon Nanotube Composites with Poly(diallyldimethylammonium chloride) for the Sensitive Detection of Hydrogen Peroxide

Yao

Shiu

2010

ANAL. SCI.

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Recently, there have been several reports integrating PB and CNT for hydrogen peroxide detection. [24][25][26] In this report, a simple procedure for the deposition of PB on the sidewalls of multi-walled carbon nanotubes (MWCNT) is explained. The resulting MWCNT-PB composite was used as a building block for the fabrication of multilayer structures on glassy carbon electrodes by the layer-by-layer technique. [27][28][29][30][31] Multilayer assemblies of MWCNT-PB composites were obtained by alternative dipping the electrode in separate solutions containing positively charged poly(diallyldimethylammonium chloride) (PDDA) and negatively charged MWCNT-PB composites. The multi-layered film showed sensitive detection for the reduction of hydrogen peroxide. The amount of MWCNT-PB deposits can be easily controlled, and the resulting material affects the sensitivity of the detection. This approach can be applied for the construction of high-performance biosensors and biofuel cells. Experimental Chemicals and reagentsMulti-walled carbon nanotubes were purchased from Nanoport Co. Ltd. (Shenzhen, China), and were treated in a 1:3 (v:v) mixture of concentrated HNO3-H2SO4 at 50 C for 24 h. The CNT materials were then thoroughly washed with doubly distilled water. The functionalized MWCNT materials were obtained by drying in vacuum at 70 C overnight. PDDA with a molecular weight range of 200000 -350000 was purchased 2010 © The Japan Society for Analytical Chemistry † To whom correspondence should be addressed. Prussian blue (PB) was deposited on multi-walled carbon nanotubes (MWCNT) in an aqueous solution. Multi-layer composites of the MWCNT-PB hybride material were obtained by layer-by-layer assembly with poly(diallyldimethylammonium chloride) (PDDA). The resulting {PDDA/MWCNT-PB}n multilayer films immoblized on glassy carbon electrodes showed sensitive detection for the reduction of hydrogen peroxide. A sensor based on the {PDDA/MWCNT-PB}n multilayer structure was fabricated and showed excellent sensitivity to the electrochemical reduction of hydrogen peroxide. Its response sensitivity increased with the number of the multilayers. A high response sensitivity of 0.83 mA M -1 cm -2 was obtained for a seven-layer sensor. This redox active multilayer structure offers potential applications in the development of high performance biosensors and biofuel cells.

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Amperometric Biosensor for Glutamate Using Prussian Blue-Based “Artificial Peroxidase” as a Transducer for Hydrogen Peroxide

Cited by 413 publications

References 26 publications

Prussian Blue based screen printed biosensors with improved characteristics of long-term lifetime and pH stability

Prussian Blue based screen printed biosensors with improved characteristics of long-term lifetime and pH stability

Amperometric l-glutamate biosensor based on bacterial cell-surface displayed glutamate dehydrogenase

Layer-by-layer Assembly of Prussian Blue and Carbon Nanotube Composites with Poly(diallyldimethylammonium chloride) for the Sensitive Detection of Hydrogen Peroxide

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