<i>Trapa japonica</i> Flerov Extract Attenuates Lipid Accumulation through Downregulation of Adipogenic Transcription Factors in 3T3-L1 Cells

Abstract

Obesity is a major human health problem associated with various diseases, including cardiac injury and type 2 diabetes. Trapa japonica Flerov (TJF) has been used in traditional oriental medicine to treat diabetes. In this study, we evaluated the inhibitory effect of and the mechanism underlying the effect of TJF extract on adipogenesis in 3T3-L1 cells. The effects of TJF extract on cell viability were analyzed using a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay, and the anti-adipogenic effect was measured by oil red O staining. The expression of peroxisomal proliferator activated receptor (PPAR)γ, CCAAT/enhancer-binding protein-α (C/EBP)α, adenosine monophosphate-activated protein kinase (AMPK), acetyl-CoA carboxylase (ACC), adiponectin, and fatty acid binding protein (FABP)4 involved in adipogenesis was determined by western blot analysis. TJF extract effectively inhibited lipid accumulation and the expression of PPARγ and C/EBPα in 3T3-L1 cells. TJF also increased the phosphorylation of AMPK and ACC, and decreased the expression of adiponectin and FABP4. These results indicate that TJF extract exerts its anti-obesity effect through the downregulation of adipogenic transcription factors and adipogenic marker genes.

Germacrone inhibits adipogenesis and stimulates lipolysis via the AMP-activated protein kinase signalling pathway in 3T3-L1 preadipocytes

“…Numerous natural products and their components have been explored for their ability to reduce adipogenesis and lipid accumulation . Recently, several studies reported that the effects of natural products and their components on adipogenesis and lipogenesis occur through down‐regulating adipogenic transcription factors and via the facilitation of glucose utilization . Therefore, it is possible that extracts and compounds from natural resources may be used as alternative therapeutic options for obesity treatment …”

“…[51][52][53][54] Recently, several studies reported that the effects of natural products and their components on adipogenesis and lipogenesis occur through down-regulating adipogenic transcription factors and via the facilitation of glucose utilization. [55][56][57][58] Therefore, it is possible that extracts and compounds from natural resources may be used as alternative therapeutic options for obesity treatment. [59] During 3T3-L1 preadipocyte differentiation, initial growth arrest is induced by a prodifferentiation hormonal regimen and this is followed by clonal expansion.…”

Inhibitory effects ofAster spathulifoliusextract on adipogenesis and lipid accumulation in 3T3-L1 preadipocytes

“…[9] Some studies have demonstrated that many natural products showed their ability to inhibit adipogenesis. [35][36][37][38] Recent papers about natural products on adipogenesis reported possible mechanisms, involving downregulation of adipogenic transcription factors [39][40][41] and facilitation of glucose utilization. [42] Therefore, it is anticipated that noble natural products, which suppress adipogenesis and lipid accumulation, may be a good candidate to treat obesity.…”

Identification of Antioxidative Hydrolyzable Tannins in Water Chestnut

“…Water chestnuts from T. japonica and T. bispinosa also reportedly contain hydrolyzable tannins [ 1 , 3 , 4 , 5 ]. Biological studies have indicated diverse health benefits associated with water chestnut, including antioxidant [ 1 , 6 ], antidiabetic [ 5 , 7 , 8 ], anti-obesity [ 9 , 10 ], anti-glycation [ 5 ], anti-inflammatory [ 11 , 12 ], antiadipogenic [ 6 ], and hepatic protective effects [ 13 ]. Furthermore, it has demonstrated inhibitory activity against α-amylase [ 14 ] and α-glucosidase [ 3 , 5 , 14 ].…”