Inhibitory effects ofAster spathulifoliusextract on adipogenesis and lipid accumulation in 3T3-L1 preadipocytes

Abstract

ASE showed anti-adipogenic and anti-lipogenic effects through the regulation of adipogenic factors and AMPKα pathway.

Germacrone inhibits adipogenesis and stimulates lipolysis via the AMP-activated protein kinase signalling pathway in 3T3-L1 preadipocytes

“…For Oil Red O staining, the cells were washed twice with phosphate‐buffered saline, fixed with 10% formalin and stained with Oil Red O (Sigma‐Aldrich, St. Louis, MO, USA) for 1 h at room temperature . The stained adipocytes were dissolved in 100% isopropanol, and the absorbance was measured at 510 nm …”

“…The whole cell lysates were obtained and centrifuged. The intracellular TG contents were analysed using an enzymatic method and calculated as the mg of TG/mg of proteins in the whole cell lysates …”

“…In our previous in‐vitro and in‐vivo studies, AS extract (ASE) showed inhibitory effects on adipogenesis/lipid accumulation in 3T3‐L1 preadipocytes and antiobesity effects in HFD‐induced obese rats . In the clinical trial, ASE also significantly reduced fat mass and induced weight loss with no obvious adverse effects .…”

“…The regulation of energy homoeostasis is a key mechanism in the regulation of overweight and obesity, in which adipose tissues play an important role as critical integrators through the regulation of both energy intake and expenditure . At the cellular level, hypertrophy and hyperplasia from immature preadipocyte cell lines characterize the process of obesity, and these two primary processes regulate adipocyte differentiation …”

“…Aster spathulifolius Maxim (AS, Asteraceae) is an edible plant that is distributed along the coasts of the south‐eastern area of South Korea . The aerial parts of AS have been used in South Korea to treat diuresis and asthma .…”