<i>Pseudomonas aeruginosa</i> BUP2—A Novel Strain Isolated from Malabari Goat Produces Type 2 Pyoverdine

Abstract

This study focuses on the isolation and characterization of a novel strain of siderophore producing bacterium, i.e., Pseudomonas aeruginosa BUP2 (Pa BUP2) from the rumen of Malabari goat, coupled with qualitative and quantitative analyses of the siderophore produced by it. Pa BUP2-a facultative anaerobe was tuned to be an aerobe by repeatedly growing in Benjamin flask. The new isolate was grown in a specially designed semi-synthetic medium, designated as BUP medium, and the yellowish-green pigment produced was identified as a typical siderophore by spectrophotometry, Chromazurol-S assay, thin layer chromatography and isolectric focusing (IEF). The characteristic orange fluorescence upon UV irradiation on chromatogram and absorption maximum at λ404 confirmed that the characteristic siderophore produced by Pa BUP2 was a typical pyoverdine (PVD). This PVD was further categorized under type 2 by comparing its profile on the IEF gel with that of the representative strains of each PVD types, viz., Pa O1, Pa ATCC 27853 and Pa6. Moreover, the type 2 PVD was purified by XAD-4 Amberlite column chromatography and quantified; maximum yield (11.17 mg/ml) was observed on day 4 of incubation (37˚C). Thus, it was confirmed that the bacterium isolated from the rumen content of Malabari goat is a novel strain of Pa capable of producing large quantity of PVD type 2 in specially designed BUP medium under aerobic condition, and that its clinical and industrial implications remain elusive.

Quorum sensing modulatory effect of sound stimulation onSerratia marcescensandPseudomonas aeruginosa

“…Pyoverdine extraction (Unni et al, 2014) Two mL of the culture broth was mixed with chloroform (Merck, Mumbai) in 2:1 proportion followed by centrifugation at 12,000 rpm (15,300 g) for 10 min. This resulted in formation of two immiscible layers.…”

Rubber Seed Kernel as Potent Solid Substrate for the Production of Lipase by Pseudomonas aeruginosa Strain BUP2

“…Pseudomonas aeruginosa strain BUP2 (MTCC No.5924), a new bacterial strain already reported from this laboratory was used throughout the study, which was isolated from the rumen of Malabari goat [6].…”

“…aeruginosa BUP2 was maintained 4˚C on mineral salt-oil-agar medium. Seed culture (12 h old) was prepared in the BUP medium by inoculating a loopful of stock culture of bacterium into it [6], which was incubated at 37˚C and 130 rpm.…”

“…Pseudomonas aeruginosa strain BUP2 (MTCC No. 5924), a new bacterial strain reported from this laboratory [6] was used in this study. Thus, the present study investigates the efficiency of the ground kernels of rubber seed, coconut and groundnut or deoiled cakes as solid substrate for the production of lipase by P. aeruginosa strain BUP2.…”

Effect of Electrical Current Stimulation on Pseudomonas Aeruginosa Growth

“…PA can catabolize a ubiquitous range of organic molecules, including organic compounds like benzoate. This, makes PA is a very ubiquitous microorganism, for it has been found in wide environmental conditions such as soil, water, humans, animals, plants, sewage, and hospitals [13] [14]. PA is the predominant inhabitant in all aquatic ecosystems, which contain high dissolved oxygen content, but its existence in low plant nutrients makes it clearly the most abundant organism [14][15] [16].…”