<i>In Vitro</i> Propagation of Endangered Orchid, <i>Vanda pumila</i> Hook.f. through Protocorms Culture

Abstract

The Vanda pumila is a monopodial orchid with beautiful flowers that are native to Thailand but now found across South Asia. The immature seeds of Vanda pumila were used for in vitro culture and then the protocorms developed were used as explants for seedling development and mass propagation. Protocorms were cultured on 1/2 MS (Murashige and Skoog, 1962) medium fortified separately with Kinetin (Kn), 6-Benzyl amino purine (BAP) and Gibberellic Acid (GA3) each in different concentrations as (0.5 mg/L, 1.0 mg/L and 2.0 mg/L) well as each on each concentrations of each medium supplemented with 5% and 10% coconut water (CW) respectively. The greatest number of shoots (9.50 ± 0.29 shoots per culture) was developed on 1/2 MS medium fortified with 1.0 mg/L Kn plus 10% CW and the longest shoots (0.78 ± 0.07 cm per culture) developed on 1/2 MS medium fortified with 2.0 mg/L BAP plus 10% CW. The shoots derived from protocorms were then developed on 1/2 MS medium fortified with three different rooting hormones viz. Indole-3-acetic acid (IAA), Indole-3-butyric acid (IBA) and α-Naphthalene acetic acid (NAA), each in four concentrations (0.5 mg/L, 1.0 mg/L, 1.5 mg/L and 2.0 mg/L) as well as 1.0 mg/L of each hormone supplemented with 10% CW. The 1/2 MS medium fortified with 0.5 mg/L IAA was found to be the most effective condition for the development of maximum number of root (5 ± 0.0 roots per culture) and root length (0.93 ± 0.07 cm). Hence, the present study could be useful for standardizing the protocol for mass propagation of the endangered orchid V. pumila.

In vitro propagation and analysis of mixotrophic potential to improve survival rate of Dolichandra unguis-cati under ex vitro conditions

“…Our results are in accordance with the studies carried out on Cichorium intybus [35], Digitalis lanata [36], Prosopis laevigata [37] and Securidaca longipedunculata [38]. The superiority of IAA over other auxins for induction of rhizogenesis has also been reported in Vanda pumila [39], Dendrobium chryseum [40], Phyllanthus tenellus [34].…”

In vitro: Influence of Various Concentrations of Plant Growth Regulators (BAP & NAA) and Sucrose on Regeneration of Chenopodium quinoa Willd. Plant

“…Thereafter, the plantlets were transferred to green house to follow their development and produced the 2 nd Generation (F2). Good effect of half-strength medium for rooting is in conformity with the results recorded for many plant as Lavendula latifolia [34], Lavendula dentata [35], Prunella Vulgaris [36] and Vanda pumila [37]. The superiority of the ½MS medium may be attributed to that the reduction in the levels of salts in the medium means a reduction in the level of nitrogen in medium, which leads to a reduction in its internal level in the shoots, this consequently may lead to an increase in the proportion of carbohydrates to nitrogen, which leads to increase the formation of roots [38,39].…”

In vitro propagation of the endangered orchid Dendrobium chryseum Rolfefrom protocorms culture

“…This result was partially supported by Luo et al, (2008), who found that 2.0 mg/L BAP was more effective than other concentrations to achieve 100% PLBs conversion of Dendrobium densiflorum shoots. Similarly, 1.0 mg/L BAP was found better in shoot multiplication (6 shoots per culture) in Vanda Pumila (Maharjan et al, 2019). Other researchers also found that BAP stimulates growth: David et al, (2008) reported that the Vanda helvola produced the highest number of shoots on a Knudson C medium supplemented with 1.0 mg/L BAP, while Goswami et al, (2015) observed that ½ MS medium plus 0.5 mg/L NAA and 0.5 mg/L BAP fostered the highest number of shoots from PLBs of Dendrobium species.…”

“…5). However, Maharjan et al, (2019) found that 1.0mg/L Kn fortified with 10% CW was the best medium for shoot multiplication in Vanda pumila. Kalpona et al, (2000) observed that Vacin and Went (V.W.)…”