Amniotic membrane and amniotic cells: Potential therapeutic tools to combat tissue inflammation and fibrosis?

Manuelpillai, Ursula; Moodley, Yuben; Borlongan, Cesario V.; Parolini, Ornella

doi:10.1016/j.placenta.2011.04.010

Cited by 137 publications

(149 citation statements)

References 44 publications

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“…More importantly, cultured AF-MSCs expressed pluripotency markers such as the Octamer binding protein 3/4 (OCT3/4) and MYC (Pan et al 2002, Tsai et al 2004, Bossolasco et al 2006, De Coppi et al 2007, Roubelakis et al 2007, which are known to maintain stem cell pluripotency and selfrenewal (Varlakhanova et al 2010). Similarly, AECs were positive for CD44, ITGB1, NT5E, ENG, ALCAM, and MHC-I and negative for CD34, CD14, PTPRC, and MHC-II expression (Miki et al 2007b, Soncini et al 2007, Manuelpillai et al 2011. Further investigation demonstrated that AECs also express embryonic stem cell markers such as POU5F1 and MYC.…”

Section: Discussionmentioning

confidence: 99%

Mesenchymal stem cells from amnion and amniotic fluid in the bovine

Corradetti¹,

Meucci²,

Bizzaro³

et al. 2013

Reproduction

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Amnion and amniotic fluid (AF) are noncontroversial and inexhaustible sources of mesenchymal stem cells (MSCs) that can be harvested noninvasively at low cost. As in humans, also in veterinary field, presumptive stem cells derived from these tissues reveal as promising candidates for disease treatment, specifically for their plasticity, their reduced immunogenicity, and high anti-inflammatory potential. The aim of this work is to obtain and characterize, for the first time in bovine species, presumptive MSCs from the epithelial portion of the amnion (AECs) and from the AF (AF-MSCs) to be used for clinical applications. AECs display a polygonal morphology, whereas AF-MSCs exhibit a fibroblastic-like morphology only starting from the second passage, being heterogeneous during the primary culture. For both lines, the proliferative ability has been found constant over the ten passages studied and AECs show a statistically lower (P!0.05) doubling time with respect to AF-MSCs. AECs express MSC-specific markers (ITGB1 (CD29), CD44, ALCAM (CD166), ENG (CD105), and NT5E (CD73)) from P1 to P3; in AF-MSCs, only ITGB1, CD44, and ALCAM mRNAs are detected; NT5E is expressed from P2 and ENG has not been found at any passage. AF-MSCs and AECs are positive for the pluripotent markers (POU5F1 (OCT4) and MYC (c-Myc)) and lack of the hematopoietic markers. When appropriately induced, both cell lines are capable of differentiating into ectodermal and mesodermal lineages. This study contributes to reinforce the emerging importance of these cells as ideal tools in veterinary medicine. A deeper evaluation of the immunological properties needs to be performed in order to better understand their role in cellular therapy.

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Section: Discussionmentioning

confidence: 99%

Mesenchymal stem cells from amnion and amniotic fluid in the bovine

Corradetti¹,

Meucci²,

Bizzaro³

et al. 2013

Reproduction

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“…Pre-clinical studies have employed the HAM successfully and showed that its use can accelerate the regeneration of various tissues 5,10,11 . However, only a limited number of studies have investigated its application as biological dressings.…”

mentioning

confidence: 99%

“…In addition, the harvesting of the amniotic membrane does not offer any risk to the newborn or the parturient, and the membrane can be easily obtained and stored in laboratories for later use. The use of these biomaterials does not raise ethical or religious conflicts, besides being a cost-effective alternative, when compared to the isolation and culture of stem cells, which require more complex processes [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18] . Therefore, the results confirm the biocompatibility of the HAM and that its application promoted a greater formation of collagen fibers and total re-epithelialization of the wound area.…”

mentioning

confidence: 99%

Effects of the application of the amniotic membrane in the healing process of skin wounds in rats

et al. 2018

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1 AbstractPurpose: To evaluate the efficacy of the application of the human amniotic membrane (HAM) on the inflammatory process, fibroblast proliferation, formation of collagenand reduction of skin wound areas in rats. Methods: Thirty six rats were submitted to a surgical injury induction and divided into two groups (n = 18): group C (control) and T (treated with the HAM). The macroscopic evolution in the wound area and the histological characteristics of the skin samples were evaluated. Results: The regression of the wound area was greater in group T. The histological analysis revealed a significant reduction (p < 0.05) in the inflammatory infiltrate in group T at all experimental periods compared with that in the control group. Furthermore, the group T presented a significant increase in the proliferation of fibroblasts at 14 and 21 days compared with group C (p < 0.05). Regarding the deposition of mature collagen fibers, there was an increase in the replacement of type III collagen by type I collagen in group T (p < 0.05). Conclusion: Treatment with the HAM reduced the healing time as well as the inflammatory responses, increased the proliferation of fibroblasts, and induced a higher concentration of mature collagen fibers.

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“…Amniotic epithelial cells (AEC) and amniotic mesenchymal stromal cells (AMSC) isolated from the amniotic membrane, the innermost layer of the placenta were known to exhibit anti-inflammatory properties [66]. Previous researches by Sur et al [9] investigated the anti-inflammatory activity of placental extracts by inducing inflammation in the hind paws of Wistar rats with carrageenan, serotonin (5-HT) and prostaglandin E 1 .…”

Section: Biological and Therapeutic Applications Of Placental Extractmentioning

confidence: 99%

Placental therapy: An insight to their biological and therapeutic properties

Pan¹,

Chan²,

Wong³

et al. 2017

J Med Therap

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The placenta, supporting the growth and development of the fetus, are rich in amino acids, peptides, vitamins, trace elements and growth factors, nutrients and biological active components. Placental therapy was applied to promote from the recovery of diseases and tissue regeneration since the early 1900s. Since then, placental therapy was applied in various clinical applications as they were shown to exhibit remarkable therapeutic attributes ranging from anti-oxidant, antimicrobial, anti-inflammatory, pain reduction, hair growth promotion, health improvement, cellular proliferation, tissue regeneration and wound healing properties in vivo. This review further summarizes the historical aspects, cellular mechanism, nutrient content and therapeutics properties of the placenta.

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Amniotic membrane and amniotic cells: Potential therapeutic tools to combat tissue inflammation and fibrosis?

Cited by 137 publications

References 44 publications

Mesenchymal stem cells from amnion and amniotic fluid in the bovine

Mesenchymal stem cells from amnion and amniotic fluid in the bovine

Effects of the application of the amniotic membrane in the healing process of skin wounds in rats

Placental therapy: An insight to their biological and therapeutic properties

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