Ammonia Monooxygenase from Nitrosomonas europaea

Arp, Daniel J.; Hommes, Norman G.; Hyman, Michael R.; Juliette, Lisa Y.; Keener, William K.; Russell, Sterling A.; Sayavedra-Soto, Luis A.

doi:10.1007/978-94-009-0213-8_22

Cited by 65 publications

(4 citation statements)

References 21 publications

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“…ple, we have demonstrated that 2-alkynes are potent inactivators of toluene-oxidizing activity in B. cepacia G4, whereas these compounds are known to be largely ineffective inactivators of both sMMO and AMO (3,7,30). Second, our observation that B. cepacia G4 can readily oxidize both ethylene and propylene supports the general observation that this activity is common to TCE-degrading strains.…”

Section: Discussionsupporting

confidence: 78%

Inactivation of Toluene 2-Monooxygenase in Burkholderia cepacia G4 by Alkynes

Yeager¹,

Bottomley

Arp³

et al. 1999

Appl Environ Microbiol

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High concentrations of acetylene (10 to 50% [vol/vol] gas phase) were required to inhibit the growth of Burkholderia cepaciaG4 on toluene, while 1% (vol/vol) (gas phase) propyne or 1-butyne completely inhibited growth. Low concentrations of longer-chain alkynes (C5 to C10) were also effective inhibitors of toluene-dependent growth, and 2- and 3-alkynes were more potent inhibitors than their 1-alkyne counterparts. Exposure of toluene-grownB. cepacia G4 to alkynes resulted in the irreversible loss of toluene- and o-cresol-dependent O2 uptake activities, while acetate- and 3-methylcatechol-dependent O2 uptake activities were unaffected. Toluene-dependent O2 uptake decreased upon the addition of 1-butyne in a concentration- and time-dependent manner. The loss of activity followed first-order kinetics, with apparent rate constants ranging from 0.25 min−1 to 2.45 min−1. Increasing concentrations of toluene afforded protection from the inhibitory effects of 1-butyne. Furthermore, oxygen, supplied as H2O2, was required for inhibition by 1-butyne. These results suggest that alkynes are specific, mechanism-based inactivators of toluene 2-monooxygenase inB. cepacia G4, although the simplest alkyne, acetylene, was relatively ineffective compared to longer alkynes. Alkene analogs of acetylene and propyne—ethylene and propylene—were not inactivators of toluene 2-monooxygenase activity in B. cepacia G4 but were oxidized to their respective epoxides, with apparentKs and V max values of 39.7 μM and 112.3 nmol min−1 mg of protein−1 for ethylene and 32.3 μM and 89.2 nmol min−1 mg of protein−1 for propylene.

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Section: Discussionsupporting

confidence: 78%

Inactivation of Toluene 2-Monooxygenase in Burkholderia cepacia G4 by Alkynes

Yeager¹,

Bottomley

Arp³

et al. 1999

Appl Environ Microbiol

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“…As for sMMO-expressing cells, formate and methanol oxidation was not affected by phenylacetylene in pMMO-expressing cells (Table 2). Such a result, although surprising, is not unprecedented, as inhibition of AMO activity by several compounds in N. europaea has been seen to be enhanced by the presence of ammonia (Arp et al, 1996). As shown in Fig.…”

Section: Differential Inhibition Of Monooxygenases By Phenyacetylene 489mentioning

confidence: 87%

“…It appears that, for phenylacetylene to inhibit pMMO activity effectively, methane must first be bound by pMMO. Such a result, although surprising, is not unprecedented, as inhibition of AMO activity by several compounds in N. europaea has been seen to be enhanced by the presence of ammonia (Arp et al, 1996). For AMO, it has been suggested that the addition of ammonia promotes the production of some reactive species that activated the inhibitors (Keener et al, 1998).…”

Section: Differential Inhibition Of Monooxygenases By Phenyacetylene 489mentioning

confidence: 99%

Differential inhibition in vivo of ammonia monooxygenase, soluble methane monooxygenase and membrane‐associated methane monooxygenase by phenylacetylene

Lontoh

DiSpirito

Krema

et al. 2000

Environmental Microbiology

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Phenylacetylene was investigated as a differential inhibitor of ammonia monooxygenase (AMO), soluble methane monooxygenase (sMMO) and membrane-associated or particulate methane monooxygenase (pMMO) in vivo. At phenylacetylene concentrations > 1 microM, whole-cell AMO activity in Nitrosomonas europaea was completely inhibited. Phenylacetylene concentrations above 100 microM inhibited more than 90% of sMMO activity in Methylococcus capsulatus Bath and Methylosinus trichosporium OB3b. In contrast, activity of pMMO in M. trichosporium OB3b, M. capsulatus Bath, Methylomicrobium album BG8, Methylobacter marinus A45 and Methylomonas strain MN was still measurable at phenylacetylene concentrations up to 1,000 microM. AMO of Nitrosococcus oceanus has more sequence similarity to pMMO than to AMO of N. europaea. Correspondingly, AMO in N. oceanus was also measurable in the presence of 1,000 microM phenylacetylene. Measurement of oxygen uptake indicated that phenylacetylene acted as a specific and mechanistic-based inhibitor of whole-cell sMMO activity; inactivation of sMMO was irreversible, time dependent, first order and required catalytic turnover. Corresponding measurement of oxygen uptake in whole cells of methanotrophs expressing pMMO showed that pMMO activity was inhibited by phenylacetylene, but only if methane was already being oxidized, and then only at much higher concentrations of phenylacetylene and at lower rates compared with sMMO. As phenylacetylene has a high solubility and low volatility, it may prove to be useful for monitoring methanotrophic and nitrifying activity as well as identifying the form of MMO predominantly expressed in situ.

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“…Moreover, N 2 production without added nitrate was independent of Mn oxide levels as rates were similar at the Mn-rich basin site and the slope site, with almost 100-fold lower Mn content. Instead, the fact that N 2 production was inhibited by both ATU and acetylene, inhibitors targeting the O 2 -dependent ammonium monooxygenase of aerobic ammonium oxidizers (B edard and Knowles 1989;Gilch et al 2009), suggests that it could be the result of aerobic nitrification coupled to denitrification, which might result from slight oxygen leakage during the incubations (De Brabandere et al 2012). We thus conclude that Mn oxidedependent ammonium oxidation did not contribute substantially to N 2 production in the UB sediments, which is consistent with results from 15 NH 1 4 incubations at other Mn-rich sites (Thamdrup and Dalsgaard 2000;Engstr€ om et al 2005;Crowe et al 2012).…”

Section: Significance Of Ramentioning

confidence: 99%

N₂ production through denitrification and anammox across the continental margin (shelf–slope–rise) of the Ulleung Basin, East Sea

Thamdrup

Kim

et al. 2017

Limnology & Oceanography

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Experimental determinations of nitrogen cycling in deep-sea sediments are strongly underrepresented in the databases. To investigate the total N 2 production rates and relative contribution of denitrification and anaerobic ammonium oxidation (anammox) to benthic fixed-N removal processes, we conducted ). The contribution of anammox to the total N 2 production (ra) increased with increasing water depth from the shelf (ca. 17%) to the basin (ca. 56%). The enhanced ra in the center of the UB was associated with an increased availability of nitrite for anammox, which was likely a result of the competitive suppression of denitrification by manganese reduction under MnO 2 -rich conditions. Our results emphasize the importance of anammox as a sink for reactive nitrogen in deep-sea sediments and contribute toward a mechanistic understanding of the factors controlling benthic reactive nitrogen loss in the ocean.

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Ammonia Monooxygenase from Nitrosomonas europaea

Cited by 65 publications

References 21 publications

Inactivation of Toluene 2-Monooxygenase in Burkholderia cepacia G4 by Alkynes

Inactivation of Toluene 2-Monooxygenase in Burkholderia cepacia G4 by Alkynes

Differential inhibition in vivo of ammonia monooxygenase, soluble methane monooxygenase and membrane‐associated methane monooxygenase by phenylacetylene

N₂ production through denitrification and anammox across the continental margin (shelf–slope–rise) of the Ulleung Basin, East Sea

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Ammonia Monooxygenase from Nitrosomonas europaea

Cited by 65 publications

References 21 publications

Inactivation of Toluene 2-Monooxygenase in Burkholderia cepacia G4 by Alkynes

Inactivation of Toluene 2-Monooxygenase in Burkholderia cepacia G4 by Alkynes

Differential inhibition in vivo of ammonia monooxygenase, soluble methane monooxygenase and membrane‐associated methane monooxygenase by phenylacetylene

N2 production through denitrification and anammox across the continental margin (shelf–slope–rise) of the Ulleung Basin, East Sea

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Product

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N₂ production through denitrification and anammox across the continental margin (shelf–slope–rise) of the Ulleung Basin, East Sea