Differential inhibition <i>in vivo</i> of ammonia monooxygenase, soluble methane monooxygenase and membrane‐associated methane monooxygenase by phenylacetylene

Lontoh, Sonny; DiSpirito, Alan A.; Krema, Cinder L.; Whittaker, Mark; Hooper, Alan B.; Semrau, Jeremy D.

doi:10.1046/j.1462-2920.2000.00130.x

Cited by 46 publications

(45 citation statements)

References 55 publications

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“…Inhibition of methanotrophic production of nitrous oxide by phenylacetylene The effect of phenylacetylene on N 2 O production was examined next. Phenylacetylene is known to act as a differential inhibitor of ammonium monooxygenase (AMO) of AOBs, sMMO, and pMMO (Lontoh et al, 2000). Since whole cell AMO activity in Nitrosomonas europaea was completely inhibited at phenylacetylene concentrations greater than 1 μM while MMO activity was not (Lontoh et al, 2000), phenylacetylene was proposed as a selective inhibitor in this study.…”

Section: Discussion Of Effect Of Amendments At King Highway Landfillmentioning

confidence: 94%

“…Phenylacetylene is known to act as a differential inhibitor of ammonium monooxygenase (AMO) of AOBs, sMMO, and pMMO (Lontoh et al, 2000). Since whole cell AMO activity in Nitrosomonas europaea was completely inhibited at phenylacetylene concentrations greater than 1 μM while MMO activity was not (Lontoh et al, 2000), phenylacetylene was proposed as a selective inhibitor in this study. To verify the effect of phenylacetylene on methanotrophic N 2 O production, two strains, Methylosinus trichosporium OB3b and Methylocystis sp.…”

Section: Discussion Of Effect Of Amendments At King Highway Landfillmentioning

confidence: 94%

“…In cells expressing pMMO, copper has also been shown to control expression up to 55 fold and to alter substrate affinity and specificity (Choi, et al, 2003;Semrau, 1998 Lontoh, 2000). Whole-cell methane oxidation by Methylomicrobium album BG8 (Type I, can only express pMMO) and Methylosinus trichosporium OB3b, (Type II, capable of expressing both sMMO and pMMO) were both enhanced with the addition of copper, but: (1) Methylomicrobium album BG8 had higher (~2x) pseudo-first order rates (V max /K s ) of CH 4 oxidation than Methylosinus trichosporium OB3b at all copper concentrations examined, and; (2) Methylosinus trichosporium OB3b expressing pMMO had a higher affinity and pseudo-first order rates of CH 4 oxidation rates than when expressing sMMO (Lontoh and Semrau, 1998;Lontoh, 2000). As a result, cells expressing pMMO appear to have a competitive advantage over cells expressing sMMO for turning over CH 4 at low concentrations.…”

Section: Coppermentioning

confidence: 99%

“…Recently, ammonia-oxidizing archaea have been demonstrated to predominate among ammoniaoxidizing prokaryotes in soils (Leininger et al, 2006) as well as in oceans (Wuchter et al, 2006). Furthermore, inhibition experiments have shown that crenarchaeal AMO (and AOA nitrification) is sensitive to AOB inhibitory compounds such as acetylene (Offre et al, 2009), suggesting that phenylacetylene, proposed as a selective inhibitor to distinguish between the activities of the ammonia oxygenase and the methane monooxygenases (Lontoh et al, 2000;Lee et al, 2009), may also be selectively effective on the activity of ammonia oxidizing archaea in landfills.…”

Section: Ammonia Oxidizing Crenarchaeotamentioning

confidence: 99%

“…Phenylacetylene, a specific inhibitor of MMO and AMO was provided in a subset of microcosms to investigate its usefulness to selectively inhibit N 2 O production. It has been shown that AMO expressing ammonia-oxidizing bacteria are completely inhibited at concentrations of phenylacetylene two orders of magnitude than methanotrophs expressing either sMMO or pMMO (Lontoh, et al, 2000). Thus, the effect of phenylacetylene was determined in soils incubated under 20 % CH 4 , 10 % O 2 , 15 % moisture content, and 25 mg-N·(kg soil) -1 .…”

Section: Methane Consumption and Nitrous Oxide Production In Soil Micmentioning

confidence: 99%

See 4 more Smart Citations

Strategies to Optimize Microbially-Mediated Mitigation of Greenhouse Gas Emissions from Landfill Cover Soils

Semrau¹,

Lee²,

Im³

et al. 2010

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The overall objective of this project, "Strategies to Optimize Microbially-Mediated Mitigation of Greenhouse Gas Emissions from Landfill Cover Soils" was to develop effective, efficient, and economic methodologies by which microbial production of nitrous oxide can be minimized while also maximizing microbial consumption of methane in landfill cover soils. A combination of laboratory and field site experiments found that the addition of nitrogen and phenylacetylene stimulated in situ methane oxidation while minimizing nitrous oxide production. Molecular analyses also indicated that methane-oxidizing bacteria may play a significant role in not only removing methane, but in nitrous oxide production as well, although the contribution of ammonia-oxidizing archaea to nitrous oxide production can not be excluded at this time. Future efforts to control both methane and nitrous oxide emissions from landfills as well as from other environments (e.g., agricultural soils) should consider these issues. Finally, a methanotrophic biofiltration system was designed and modeled for the promotion of methanotrophic activity in local methane "hotspots" such as landfills. Model results as well as economic analyses of these biofilters indicate that the use of methanotrophic biofilters for controlling methane emissions is technically feasible, and provided either the costs of biofilter construction and operation are reduced or the value of CO 2 credits is increased, can also be economically attractive. 3 TABLE OF CONTENTSABSTRACT 2 EXECUTIVE SUMMARY 6

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Section: Discussion Of Effect Of Amendments At King Highway Landfillmentioning

confidence: 94%

Section: Discussion Of Effect Of Amendments At King Highway Landfillmentioning

confidence: 94%

Section: Coppermentioning

confidence: 99%

Section: Ammonia Oxidizing Crenarchaeotamentioning

confidence: 99%

Section: Methane Consumption and Nitrous Oxide Production In Soil Micmentioning

confidence: 99%

See 3 more Smart Citations

Strategies to Optimize Microbially-Mediated Mitigation of Greenhouse Gas Emissions from Landfill Cover Soils

Semrau¹,

Lee²,

Im³

et al. 2010

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Aerobic Methanotrophy and Nitrification: Processes and Connections

Stein

Roy

Dunfield

2012

Encyclopedia of Life Sciences

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Ammonia and methane are structurally similar molecules. Not surprisingly therefore, microorganisms that use methane as a sole energy source (methanotrophs) and microorganisms that use ammonia as a sole energy source (ammonia oxidisers or nitrifiers) share many similarities. They have several key enzymes in common, most especially the ammonia monooxygenase/particulate methane monooxygenase enzyme family. The two groups are proposed to have a common evolutionary history. They occupy similar ecological niches, and compete for nitrogen. Enzymatically, nitrifiers are capable of methane oxidation, and methanotrophs are capable of nitrification. Microbial ecologists have attempted to find specific inhibitors for either group in order to study their respective roles in the environment. The contribution of ammonia oxidisers to methanotrophy in natural systems appears to be very minor, however methanotrophs may sometimes have important roles in the nitrogen cycle. Key Concepts: Some bacteria and archaea are capable of using methane or ammonia as energy sources. Methanotrophs and ammonia oxidisers are each highly specialised to living on their particular substrate. Methanotrophs and ammonia oxidisers have several key enzymes in common, and may share a common evolutionary history. Both groups must cope with toxic by‐products of ammonia oxidation. Methanotrophs may be important in the environmental nitrogen cycle, but ammonia oxidisers do not affect the methane cycle. Ammonia oxidising archaea appear to outcompete ammonia oxidising bacteria under ammonia‐limiting and acidic conditions.

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Biopolymers made from methane in bioreactors

Chidambarampadmavathy

Karthikeyan

Heimann

2015

Engineering in Life Sciences

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Methane (CH4) is a potent greenhouse gas and mitigation is important to reduce global warming impacts. In this study, we aimed to convert CH4 to polyhydroxybutyrate (PHB; a biopolymer) by enrichment of methanotrophic consortia in bioreactors. Two different methanotrophic consortia were established form landfill top‐cover (landfill biomass [LB]) and compost soils (compost biomass [CB]), through cultivation under CH4:CO2:air (30:10:60) in batch systems. The established cultures were then used as inoculi (0.5 g LB or CB⋅L−1) in continuous stirred tank reactors (CSTRs) aerated with CH4:CO2:air at 0.25 L⋅min−1. Under stable CSTRs operating conditions, the effect of spiking with 1:1 copper:iron (final concentrations of 5μM) was tested. Methane oxidation capacity (MOC), biomass dry weight (DWbiomass), PHB, and fatty acid methyl esters (FAMEs) contents were used as effect parameters. A maximum MOC of 481.9 ± 8.9 and 279.6 ± 11.3 mg CH4⋅g−1 DWbiomass⋅h−1 was recorded in LB‐CSTR and CB‐CSTR, respectively, but PHB production was similar for both systems, that is 37.7 mg⋅g−1 DWbiomass. Treatment with copper and iron improved PHB production (22.5% of DWbiomass) in LB‐CSTR, but a reduction of 13.6% was observed in CB‐CSTR. The results indicated that CH4 to PHB conversion is feasible using LB‐CSTRs and addition of copper and iron is beneficial.

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Differential inhibition in vivo of ammonia monooxygenase, soluble methane monooxygenase and membrane‐associated methane monooxygenase by phenylacetylene

Cited by 46 publications

References 55 publications

Strategies to Optimize Microbially-Mediated Mitigation of Greenhouse Gas Emissions from Landfill Cover Soils

Strategies to Optimize Microbially-Mediated Mitigation of Greenhouse Gas Emissions from Landfill Cover Soils

Aerobic Methanotrophy and Nitrification: Processes and Connections

Biopolymers made from methane in bioreactors

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