Aminopeptidase in Human Seminal Plasma

Abstract

Aminopeptidase of seminal plasma was studied in normozoospermic, oligozoospermic, obstructive and non-obstructive azoospermic men. Significantly higher activity was observed in non-obstructive azoospermic patients than that of other three groups. L-Alanine P-naphthylamide hydrochloride was found to have highest hydrolysis rate in comparison to various other aminoacyl 0-naphthylamide hydrochlorides which were used for determining substrate specificity. 5% inhiiitions of the enzyme activity by EDTA and 1 ,lo-phenanthroline were obtained at 5.77 X M concentrations respectively. A single band for aminopeptidase in the activitygel electrophoresis was obtained for all the categories of seminal plasma and the enzyme exhibited the characteristics of metalloprotein.M and 3.13 X Aminopeptidase im menschlichen Spermaplasma

Insemination extender supplementation with bestatin and EDTA has no effect on rabbit reproductive performance

“…APN activity has been inhibited in animal sperm with different substances such as bestatin [13][14][15][16], Ethylenediaminetetraacetic acid (EDTA) [17][18][19][20], or both [21][22].…”

Development of new artificial insemination extenders supplemented with GnRH analogues to induce ovulation and proteomic characterization of rabbit semen

“…In previous works, the bioavailability of buserelin acetate when added to the seminal dose appeared to be determined by the seminal plasma aminopeptidase activity APN activity has been inhibited in animal sperm with different substances such as bestatin (Togo and Morisawa, 2004;Kubo et al, 2008;Yasuhara et al, 1983;, Ethylenediaminetetraacetic acid (EDTA) (Nag Das et al, 1984Das et al, , 1988, or both (Huang et al, 1997;Marinho et al, 2008). This inhibition can affect different fertilization steps depending on the species considered.…”