Development of new artificial insemination extenders supplemented with GnRH analogues to induce ovulation and proteomic characterization of rabbit semen

Abstract

Ammonium bicarbonate ACN Acetonitrile AI Artificial insemination ALH Mean amplitude of lateral head displacement AMIs Aminopeptidase inhibitors ANOVA Analysis of variance APN Aminopeptidase activity AEBSF 4-(2-Aminoethyl) benzenesulfonyl fluoride hydrochloride BCA Bicinchoninic acid method BCF Frequency of head displacement BSA Bovine serum albumin ºC Celsius degrees CL Corpus lutea cm Centimetre cps Counts per second CS Chitosan eCG Equine chorionic gonadotropin EDTA Ethylenediaminetetraacetic acid ELISA Enzyme-linked immunosorbent assay EU European Union DA Discriminant Analysis DE Digestible energy DM Dry matter DS Dextrane sulfate DTT Dithiothreitol FA Formic acid FDR False discovery rate FITC-PNA Fluorescein labeled lectin from the peanut plant Arachis hypogaea fmol Femtomole V Volt VAP Average path velocity VCL Average of curvilinear velocity vol Volume z Charge number of ions β-NGF Beta-nerve growth factor 1D One-dimension µ Micro µg Micrograms µL Microlitres µM Micromolar µm Micrometre µmol Micromol I.GENERAL INTRODUCTION European Social Fund. IV.CHAPTER II IV.CHAPTER II Insemination extender supplementation with bestatin and EDTA has no effect on rabbit reproductive performance