Amino covalent binding approach on iron oxide nanoparticle surface: Toward biological applications

Abstract

ABSTRACT:We report on the synthesis and the surface modification of different types of magnetic iron oxide particles by developing an original process based on diazonium salts chemistry. Particles were first coated with amino groups and then subjected to polyethylene glycol (PEG) surface modification. They were subsequently characterized by Transmission electron microscopy, infrared spectroscopy, diffraction light scattering and by Zeta potential. To show the efficiency of this surface modification method, the potential cytotoxicity and (pro-)inflammatory effect of the PEG magnetic particles were also analyzed in vitro. This covalently surface modification approach based on diazonium salts chemistry provides individually dispersed, PEG-modified magnetic nanoparticles suitable for biological applications.

The convenient preparation of stable aryl-coated zerovalent iron nanoparticles

“…Functionalized magnetic nanoparticles (NPs) have aroused great interest recently due to their unique properties and the possibility of widespread applications [ 1 – 2 ]. The modification of magnetic materials may solve a number of high priority problems in medicine and pharmacology [ 3 ].…”

Cytotoxicity-Related Bioeffects Induced by Nanoparticles: The Role of Surface Chemistry

“…Both pristine and PEG-decorated iron oxide NPs (10 nm) could induce the release of proinflammatory chemokine IL-8 in A549 cells. The secretion level of IL-8 induced by PEG decoration was low compared to that induced by pristine iron oxide NPs (Griffete et al, 2012). In a 28 days repeated dose toxicity study, pristine-MWCNTs caused higher levels of inflammation in lung and liver of mice than PEG-MWCNTs (Zhang et al, 2017).…”

Uptake and Intracellular Fate of Peptide Surface-Functionalized Silica Hybrid Magnetic Nanoparticles In Vitro

“…Cytotoxicity : The ability for the SHMNPs‐PEG‐cRGD‐NLS to cause a cytotoxic effect upon the J774A.1 macrophage and HeLa cells was assessed by quantifying the level of lactate dehydrogenase (LDH) release, a cytosolic enzyme indicative of cell membrane damage. Both cell types were exposed to the SHMNPs‐PEG‐cRGD‐NLS at 20 and 100 μg mL −1 for 24 h. The level of LDH in the cell supernatant was assessed via a diagnostic kit (Roche, Switzerland), as previously described in Griffete et al LDH data are presented as the mean ± standard error of the mean (SEM) ( n = 3 in triplicate). Statistical significance was assessed by using a Students t ‐test (R statistical programme; r‐project.org).…”