Amino acids determination using capillary electrophoresis with on-capillary derivatization and laser-induced fluorescence detection

Veledo, Maria Teresa; Frutos, Mercedes de; Diez-Masa, Jose Carlos

doi:10.1016/j.chroma.2005.03.111

Cited by 54 publications

(34 citation statements)

References 35 publications

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“…The optimization of the in-capillary derivatization using FQ was reported by Veledo et al [25]. These workers applied the optimized conditions to a sample that contained 16 AAs.…”

Section: In-capillary Derivatizationmentioning

confidence: 97%

Recent advances in amino acid analysis by CE

et al. 2007

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This paper describes a number of articles that have been published on amino acid (AA) analysis using CE during the period from June 2005 to May 2007. This review article follows the format of the previous articles of Smith (Electrophoresis 1999, 20, 3078-3083), Prata et al.. (Electrophoresis 2001, 22, 4129-4138), and Poinsot et al.. (Electrophoresis 2003, 24, 4047-4062 and Electrophoresis 2006, 27, 176-194). Several new developments in AA analysis with CE are reported describing the use of laser-emitting diodes for LIF, MS, and chips. In addition, we describe articles concerning clinical studies and neuroclinical applications.

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“…The optimization of the in-capillary derivatization using FQ was reported by Veledo et al [25]. These workers applied the optimized conditions to a sample that contained 16 AAs.…”

Section: In-capillary Derivatizationmentioning

confidence: 97%

Recent advances in amino acid analysis by CE

et al. 2007

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“…We are currently witnessing successful applications of zone-passing capillary derivatization CE, after its first introduction by Honda et al [37] for amino acid analysis. Using the zone-passing capillary derivatization, Diez-Masa and co-workers [38] developed a CE-LIF method for the analysis of free amino acids. Several parameters that control in-capillary derivatization of amino acids, including pH, mixing time, reaction time, concentration of the derivatization reagents, 3-(2-furoyl)quinoline-2-carboxaldehyde (FQ) and KCN, and solvent of FQ, as well as the temperature of mixing and reaction were optimized.…”

Section: In-capillary Derivatization By Emmamentioning

confidence: 99%

Advances in CE‐mediated microanalysis: An update

2007

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This review, as a continuation of two earlier reports, gives an overview of the recent developments, over the period from 2005 until now, in the use of electrophoretically mediated microanalysis (EMMA) methodology for the on-line study of enzymatic reaction and derivatization. The article is divided into two parts: (i) on-line enzymatic reaction by EMMA and (ii) on-line derivatization by EMMA. Following a brief introduction, a literature overview on enzymatic reaction is provided. The second part starts with an introduction of the purpose of derivatization and the nomenclature used in the area of in-capillary derivatization based on EMMA. The development of more integrated analytical platform that combines in-capillary derivatization and sample preconcentration is discussed. Reported derivatization procedures are summarized.

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“…As known, Taurine (Tau) has a large amount in retinal tissue and governs the regulation of osmotic pressure [7][8][9], the development and maintenance of photoreceptive cells [9], and retinal degeneration [8,9]. Meanwhile, other AAs have demonstrated to be markers for certain diseases [1,10,11]. For example, the increase of free glycine (Gly) levels in plasma is an indication of propionic acidemia, a frequent inborn error of organic acid metabolism in humans [10,11].…”

Section: Introductionmentioning

confidence: 98%

“…Meanwhile, other AAs have demonstrated to be markers for certain diseases [1,10,11]. For example, the increase of free glycine (Gly) levels in plasma is an indication of propionic acidemia, a frequent inborn error of organic acid metabolism in humans [10,11]. Therefore, the determination of physiological AAs and catecholamines would be of strong significance in the clinical diagnosis and achieving better understanding of their physiological roles in biological science.…”

Section: Introductionmentioning

confidence: 98%

Separation of free amino acids and catecholamines in human plasma and rabbit vitreous samples using a new fluorogenic reagent 3‐(4‐bromobenzoyl)‐2‐quinolinecarboxaldehyde with CE‐LIF detection

Zhang

Wang

2009

Electrophoresis

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A sensitive and efficient analysis of amino acids and catecholamines is currently presented with 3-(4-bromobenzoyl)-2-quinolinecarboxaldehyde as a fluorogenic derivatization reagent using CE separation with LIF detection. For good derivatization conditions, the reagent concentrations, pH value, temperature, and reaction time were explored, which were followed by the derivatization reaction in stable yield. The optimal running buffer was composed of mixtures involving 120 mM, pH 9.1 boric acid, 38.5 mM SDS, and 19% ACN v/v. The LOD (S/N=3) was found as low as 0.65 nM. The proposed method was validated by the two-order-magnitude linearity and correlation coefficient ranging from 0.9957 to 0.9998. The accuracy and specificity of this assay were also assured from the spiking of real samples with a standard known concentration. In order to demonstrate its wide range of applications, the method has been applied to the analysis of both human plasma and rabbit vitreous samples.

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Amino acids determination using capillary electrophoresis with on-capillary derivatization and laser-induced fluorescence detection

Cited by 54 publications

References 35 publications

Recent advances in amino acid analysis by CE

Recent advances in amino acid analysis by CE

Advances in CE‐mediated microanalysis: An update

Separation of free amino acids and catecholamines in human plasma and rabbit vitreous samples using a new fluorogenic reagent 3‐(4‐bromobenzoyl)‐2‐quinolinecarboxaldehyde with CE‐LIF detection

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