Amino-acid sequence of rusticyanin from Thiobacillus ferrooxidans and its comparison with other blue copper proteins

First Evidence for Existence of an Uphill Electron Transfer through the bc 1 and NADH-Q Oxidoreductase Complexes of the Acidophilic Obligate Chemolithotrophic Ferrous Ion-Oxidizing Bacterium Thiobacillus ferrooxidans

“…It has been suggested that an uphill electron transfer, established at the expense of the energy derived from the oxidation of Fe 2ϩ by oxygen, may be involved in the reduction of NAD(P) ϩ from Fe 2ϩ (20). The electron transfer chain from Fe 2ϩ to O 2 is now thought to involve a Fe 2ϩ cytochrome c oxidoreductase (8,19), rusticyanin (12,13,28), at least one cytochrome c (the 14-kDa soluble cytochrome c [30] and/or the c 4 cytochrome [9], which shows a strong affinity for the membrane), and a cytochrome c oxidase (22). In addition, two membrane-bound c-type cytochromes with apparent molecular masses of 46 and 30 kDa have been isolated from T. ferrooxidans (15).…”

Redox Components of Cytochrome bc-type Enzymes in Acidophilic Prokaryotes

“…The presence of ubiquinone-8 has also been reported (8). Some of these compounds have been isolated and purified: a soluble 14-kDa cytochrome c 552 (9), a soluble c 4 -type cytochrome (10), the blue copper protein rusticyanin (11)(12)(13), an iron-sulfur protein Fe 2ϩ -cytochrome c 552 oxidoreductase (14,15), an a 1 -type cytochrome oxidase (16), and three membrane-bound cytochromes c: (a high molecular mass cytochrome c, the size of which depends on the strain (17)(18)(19), and a 30-kDa (19) and a 22.3-kDa (18) or 21-kDa (17,19) cytochrome c.…”

Respiratory Isozyme, Two Types of Rusticyanin ofAcidithiobacillus ferrooxidans

“…21) Later, the complete gene was cloned from strain ATCC 33020 22) and found to be comprised of 564 bp, encoding 155 amino acids, with additional codons encoding a 32-amino acid signal peptide. [21][22][23][24] The primary structure of the rus gene was found to be the same for both genes. 22) Furthermore, rus was shown to be situated downstream of genes for C-type cytochrome and cytochrome oxidase (COX ) 22) and these three have been thought to represent an operon present as a single copy within a cluster of genes encoding electron transfer proteins.…”