Amino-acid sequence of glycoproteins encoded by three alleles of the S locus of Brassica oleracea

Nasrallah, June B.; Kao, Teh Hui; Chen, C. H.; Goldberg, Michael L.; Nasrallah, Mikhail E.

doi:10.1038/326617a0

Cited by 235 publications

(155 citation statements)

References 8 publications

Supporting

Mentioning

152

Contrasting

Unclassified

Order By: Relevance

“…However, great progress is at present being made in understanding the nature of self-incompatibility alleles in both sporophytic and gametophytic systems (Nasrallah et a!., 1985(Nasrallah et a!., , 1987Takayama et a!., 1987;Anderson et a!., 1986). It may therefore soon be possible to do more than just speculate about their origin.…”

Section: Discussionmentioning

confidence: 99%

“…The alleles that have so far been sequenced in Brassica, (Nasrallah et a!., 1987;Takayama et a!., 1987) which has a sporophytic system, differ by several amino acids. This is what one would expect to find if the different alleles evolved from an ancestral inactive allele, assuming that the only alleles likely to invade the population would be ones with new S specificities not already present (Charlesworth and Charlesworth, 1979b); thus new alleles arising by mutation would be unlikely to spread if they occurred in alleles that were already active in the population, but an allele derived from S by changing a different amino acid site from those present in existing alleles could invade, provided that it produced a protein with an S specificity.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Evolution of homomorphic sporophytic self-incompatibility

Charlesworth

1988

Heredity

View full text Add to dashboard Cite

A population genetic model is described for the evolution of sporophytic self-incompatibility by successive mutations to active incompatibility alleles from an ancestral compatibility, Sf, allele, homozygotes for which are capable of selffertilisation. For spread of the active incompatibility alleles there must be strong inbreeding depression. It is shown that polymorphism for the ancestral allele and the active alleles is generated, unless the number of active alleles becomes very large. Thus, although there is selection for alleles that cause outcrossing, such polymorphic populations would not be completely outcrossing, but would have selfing rates between zero and the selfling rate of the homozygote for S1. The equilibrium frequencies of the active alleles depend on whether dominance or independent action is assumed for the alleles in the pistil. In either case the equilibrium allele frequencies are higher than would be the case for gametophytically acting alleles.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Evolution of homomorphic sporophytic self-incompatibility

Charlesworth

1988

Heredity

View full text Add to dashboard Cite

show abstract

“…charlesworth@ed.ac.uk ©1998 The Genetical Society of Great Britain. incompatibility types of plants in families, and encode sequences of cosegregating pistil proteins (Anderson et al, 1989;Li et al, 1995;Nasrallah et al, 1987;Walker et al, 1996). An important result from sequencing studies is that selfincompatibility loci have evolved from independent origins several times in flowering plants.…”

Section: Evolution and Maintenance Of Self-incompatibilitymentioning

confidence: 99%

Flowering plant self-incompatibility: the molecular population genetics of Brassica S-loci

Charlesworth

Awadalla

1998

Heredity

View full text Add to dashboard Cite

Self-incompatibility systems in different angiosperm families are reviewed, and the evidence that incompatibility has arisen several times is outlined. New data on the sequence polymorphism of self-incompatibility loci from two different angiosperm families are compared with results from other highly polymorphic loci, particularly MHC loci. We discuss what molecular genetic analyses of these sequences can tell us about the nature and maintenance of the polymorphism at self-incompatibility loci. We suggest that there is evidence for recombination at the Brassica selfincompatibility loci, so that it may be possible to discern regions that are particularly functionally important in the recognition reaction, even though the long-term maintenance of polymorphisms in these amino acid residues has caused the evolution of many other sequence differences between alleles.

show abstract

“…As expected for genes involved in self-recognition, the SRK and SCR genes are highly polymorphic (Stein et al, 1991;Kusaba et al, 1997;Kusaba and Nishio, 1999;Schopfer et al, 1999;Schopfer and Nasrallah, 2000; and appear to have coevolved. A third highly polymorphic gene contained within the S locus in most Brassica S haplotypes is the SLG (for S locus glycoprotein) gene (Nasrallah et al, 1987;Kusaba et al, 1997). This gene exhibits a high degree of sequence similarity to the extracellular domain or the S domain of SRK (Stein et al, 1991;Kusaba et al, 1997) and encodes a glycoprotein specific to the papillar cell and localized in its wall (Kandasamy et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

Self-Incompatibility in the Genus Arabidopsis: Characterization of the S Locus in the Outcrossing A. lyrata and Its Autogamous Relative A. thaliana

Kusaba¹,

Dwyer²,

Hendershot³

et al. 2001

The Plant Cell

131

282

View full text Add to dashboard Cite

As a starting point for a phylogenetic study of self-incompatibility (SI) in crucifers and to elucidate the genetic basis of transitions between outcrossing and self-fertilizing mating systems in this family, we investigated the SI system of Arabidopsis lyrata . A. lyrata is an outcrossing close relative of the self-fertile A. thaliana and is thought to have diverged from A. thaliana ‫ف‬ 5 million years ago and from Brassica spp 15 to 20 million years ago. Analysis of two S (sterility) locus haplotypes demonstrates that the A. lyrata S locus contains tightly linked orthologs of the S locus receptor kinase ( SRK ) gene and the S locus cysteine-rich protein ( SCR ) gene, which are the determinants of SI specificity in stigma and pollen, respectively, but lacks an S locus glycoprotein gene. As described previously in Brassica , the S haplotypes of A. lyrata differ by the rearranged order of their genes and by their variable physical sizes. Comparative mapping of the A. lyrata and Brassica S loci indicates that the S locus of crucifers is a dynamic locus that has undergone several duplication events since the Arabidopsis-Brassica split and was translocated as a unit between two distant chromosomal locations during diversification of the two taxa. Furthermore, comparative analysis of the S locus region of A. lyrata and its homeolog in self-fertile A. thaliana identified orthologs of the SRK and SCR genes and demonstrated that self-compatibility in this species is associated with inactivation of SI specificity genes. INTRODUCTIONSelf-incompatibility (SI) is the major outcrossing mechanism in the family Brassicaceae (de Nettancourt, 1977). Species in this family have been grouped into 19 tribes on the basis of morphological criteria (Schultz, 1936), and SI has been described in all tribes analyzed to date. When Bateman (1955) surveyed 182 species distributed in 11 tribes, he found that approximately half of these species included selfincompatible accessions. In a survey of 59 taxa in the subtribe Brassicineae of the tribe Brassiceae (which includes Brassica and Raphanus ), 50 taxa were self-incompatible (Takahata and Hinata, 1980). In all cases analyzed, SI has been shown to be controlled sporophytically by a single S (sterility) locus, with multiple alleles or variants and complex dominance relationships between alleles (Bateman, 1954(Bateman, , 1955Thompson and Taylor, 1966): in self-incompatible plants, pollen will not develop on a stigma that expresses the same S alleles as the pollen parent.Molecular analysis of the Brassica S locus region has shown that this mendelian locus is a gene complex consisting of distinct stigma-expressed and anther-expressed genes that determine SI specificity in stigma and pollen, respectively (reviewed in Nasrallah, 2000). The SRK (for S locus receptor kinase) gene (Stein et al., 1991) encodes a plasma membrane-spanning receptor serine/threonine kinase specific to the stigma epidermis (Stein et al., 1996) and is the determinant of SI specificity in the stigma (Takasaki et al., 2000). ...

show abstract

Amino-acid sequence of glycoproteins encoded by three alleles of the S locus of Brassica oleracea

Cited by 235 publications

References 8 publications

Evolution of homomorphic sporophytic self-incompatibility

Evolution of homomorphic sporophytic self-incompatibility

Flowering plant self-incompatibility: the molecular population genetics of Brassica S-loci

Self-Incompatibility in the Genus Arabidopsis: Characterization of the S Locus in the Outcrossing A. lyrata and Its Autogamous Relative A. thaliana

Contact Info

Product

Resources

About