Amino acid sequence of cinnamomin, a new member of the elicitin family, and its comparison to cryptogein and capsicein

Abstract

The phytopathogenic fungi Phytophthora cinnamom cause systemic leaf necrosis on its non-host tobacco; in culture, it secretes a protein, called cinnamomin, which elicits leaf necrosis and protects tobacco against the pathogen Phytophthora nicotiam, in a way similar to cryptogein and different from capsicem, elicitms of known amino acid sequences. The cinnamomin sequence has been determined and compared to other elicitins. The differences in the 3 elicitin sequences were correlated to the biological acivities: 2 lysines were ascribed as the key amino actds involved in the differential control of protection with respect to necrosis.Amino acrd sequence; Elicitin; Plant pathogen; (Phytophthora, Nicotiana rabacum)

Highly specific and sensitive non-radioactive molecular identification of Phytophthora cinnamomi

“…The cinnamomin protein is a polypeptide secreted by P. cinnamomi which belongs to a family of proteins designated as elicitins. It was shown to induce a hypersensitive response in tobacco (Huet & Pernollet, 1989). To target the corresponding coding DNA we had to use degenerated primers chosen on the basis of the codon usage for Phytophthora.…”

“…Based on the published aminoacid sequence of cinnamomin (Huet & Pernollet, 1989) and taking the Phytophthora codon usage into account, we defined a pair of degenerated oligonucleotides for the PCR amplification of a 257 bp DNA fragment, using the approach described in Materials and Methods. The nucleotide sequences of these primers (94.239, 95.374) and their corresponding aminoacid sequence are shown in Fig.…”

“…Some of these proteins were shown to act as inducers of a hypersensitive-like response in tobacco and radish (Kamoun et al, 1993). The aminoacid sequence of the cinnamomin protein has been recently determined (Huet & Pernollet, 1989). We Mycol.…”

“…Our approach has been to target the cinnamomin gene of P. cinnamomi, a polypeptide which belongs to a family of proteins designated as elicitins (Huet & Pernollet, 1989). Some of these proteins were shown to act as inducers of a hypersensitive-like response in tobacco and radish (Kamoun et al, 1993).…”

Effects of Growth Phase and Cell Density on Cryptogein-induced Programmed Cell Death in Suspension-cultured Tobacco BY-2 Cells: Development of a Model System for 100% Efficient Hypersensitive Cell Death

“…Because each cell is in direct contact with the culture medium, a uniform response to externally added agents is expected. Cryptogein is a proteinaceous elicitor derived from the oomycete Phytophthora cryptogea (Huet and Pernollet 1989), and it is known to induce defense responses and cell death in cultured tobacco cells, including BY-2 (Binet et al 2001, Lecouriex-Ouaked et al 2000. Cryptogein is known to trigger transient increases in cytosolic calcium ion concentration that are followed by activation of mitogen-activated protein kinases (MAPKs), production of reactive oxygen species, activation of defense-related genes, and cell death in BY-2 cells (Kadota et al 2004a, 2004b, Kadota and Kuchitsu 2006.…”