Amino acid sequence and biological activity of a γ-conotoxin-like peptide from the worm-hunting snail Conus austini

Zugasti–Cruz, Alejandro; Maillo, María Aracelis; López‐Vera, Estuardo; Falcón, Andrés; Cotera, Edgar P. Heimer de la; Olivera, Baldomero M.; Aguilar, Manuel B.

doi:10.1016/j.peptides.2005.07.021

Cited by 27 publications

(20 citation statements)

References 18 publications

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“…On the other hand, worm-hunting species have been rarely studied, even if recent studies have reported a variety of novel conopeptides, some of them with potent neuropharmacological activity present only in vermivorous cone snails [35][36][37].…”

Section: Discussionmentioning

confidence: 99%

Assessment of Biochemical and Histopathological Effects of Crude Venom of Cone Snail Conus flavidus on albino Mice

Abou-Elezz¹

2017

IJEE

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Abstract:The genus Conus is equipped with a unique venomous mixture of conopeptides which secreted for predation and defense purposes. This work is aiming to explore and determine the effect of the crude venom of Conus flavidus, a wormhunting cone snail inhabiting the Red Sea, on the oxidant/ antioxidant system in mice using some oxidative stress biomarker assays. In addition to assess its histopathological effects on some treated organs. The LC 50 were detected for the crude venom using the hemolytic assay (16.7 mg/ml) and male albino mice were injected intraperitoneally with ½ LC 50 (8.3 mg/kg B.Wt). Biochemically, after 2, 4, 6, 12, 24 hours of injection the results revealed significant inhibition of superoxide dismutase (SOD) and catalase (CAT) activities in blood and liver in almost all time intervals comparing with control one. However, it showed elevation in lipid peroxide content (LPC), protein carbonyl content (PCC), nitric oxide level (NO) reduced glutathione content (GSH) and total antioxidant capacity (TAC) contents of both blood and liver in almost all time intervals. Histopathologicaly, liver and heart were dissected after 1, 3 and 7 days of injection. The treated liver showed vacuolar degeneration, karyolosis and pyknosis, mild blood sinusoidal congestion and centrilobular necrosis. The treated heart illustrated degenerated myofibrils, pyknosis, edema, blood vessel congestion, loss of striation normal construction and fascicular pattern in the myocardium. These results revealed that C. flavidus crude venom has distinct effects upon the oxidant/antioxidant cellular system and degenerative pathological effects in some tissues of treated animals, proving that this venom may contain bioactive peptides, which could be purified and used for further pharmacological and drug discovery investigations in the future.

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Section: Discussionmentioning

confidence: 99%

Assessment of Biochemical and Histopathological Effects of Crude Venom of Cone Snail Conus flavidus on albino Mice

Abou-Elezz¹

2017

IJEE

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“…It is probable that these toxins are α-conotoxins, which inhibit neuronal homomeric and heteromeric nicotinic receptors (31,32,35). This toxin type has not been previously isolated from C. austini, since the only reported study performed on this snail venom allowed the purification of a γ-conotoxin-like peptide that belongs to the O-conotoxin superfamily (37).…”

Section: Discussionmentioning

confidence: 99%

“…Although these three species had been previously subjected to Dis., 2008, 14, 3, p. 499 biochemical studies (1,2,(21)(22)(23)37), neither the extracts prepared from their venom ducts, nor the peptides that were isolated and characterized had been evaluated in a functional model that can provide some insight into the action mechanism that mediates the pharmacological effects of their venoms. Figure 1.…”

Section: Introductionmentioning

confidence: 99%

Pharmacological characterization of venoms obtained from Mexican toxoglossate gastropods on isolated guinea pig ileum

Rojas¹,

Feregrino²,

Ibarra-Alvarado³

et al. 2008

J. Venom. Anim. Toxins incl. Trop. Dis

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ABSTRACT:The protein-containing extracts prepared from the venom ducts of Conus austini, Conus spurius and Polystira albida caused a concentration-dependent inhibition of spontaneous contractions in guinea pig ileum. The most potent extract was obtained from P. albida venom ducts (IC 50 = 0.11 ± 0.02 µg protein/mL). The three extracts produced a moderate inhibition of contractions elicited by acetylcholine (ACh 1 µM), suggesting the presence of anticholinergic compounds. The contractile response elicited by nicotine (10 µM) was significantly reduced by the extracts prepared from the ducts of C. austini and P. albida, which indicates that the venom produced by these species contains toxins that target neuronal nicotinic receptors. All three extracts significantly inhibited contractions evoked by histamine (0.5 µM), particularly those from C. spurius and P. albida. These findings reveal the presence of antihistaminergic compounds not previously described in any conoidean venom. Finally, we found that only the extract prepared from C. spurius ducts decreased KCl (60 mM)-induced contractions, indicating that the venom of this snail contains compounds that block voltage-dependent Ca 2+ or Na + channels.

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“…10) This also suggests that γ-conotoxins in the vermivorous cone snail venom including fu6a may have some roles in worm hunting. Further investigation of the biological activity of this unique toxin will reveal its biological role in the venom.…”

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confidence: 99%

Characterization of the venom of the vermivorous cone snail Conus fulgetrum

Abdel-Wahab

Miyashita

Kitanaka

et al. 2016

Bioscience, Biotechnology, and Biochemistry

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Amino acid sequence and biological activity of a γ-conotoxin-like peptide from the worm-hunting snail Conus austini

Cited by 27 publications

References 18 publications

Assessment of Biochemical and Histopathological Effects of Crude Venom of Cone Snail <i>Conus flavidus</i> on <i>albino</i> Mice

Assessment of Biochemical and Histopathological Effects of Crude Venom of Cone Snail <i>Conus flavidus</i> on <i>albino</i> Mice

Pharmacological characterization of venoms obtained from Mexican toxoglossate gastropods on isolated guinea pig ileum

Characterization of the venom of the vermivorous cone snail Conus fulgetrum

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Amino acid sequence and biological activity of a γ-conotoxin-like peptide from the worm-hunting snail Conus austini

Cited by 27 publications

References 18 publications

Assessment of Biochemical and Histopathological Effects of Crude Venom of Cone Snail &lt;i&gt;Conus flavidus&lt;/i&gt; on &lt;i&gt;albino&lt;/i&gt; Mice

Assessment of Biochemical and Histopathological Effects of Crude Venom of Cone Snail &lt;i&gt;Conus flavidus&lt;/i&gt; on &lt;i&gt;albino&lt;/i&gt; Mice

Pharmacological characterization of venoms obtained from Mexican toxoglossate gastropods on isolated guinea pig ileum

Characterization of the venom of the vermivorous cone snail Conus fulgetrum

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Assessment of Biochemical and Histopathological Effects of Crude Venom of Cone Snail <i>Conus flavidus</i> on <i>albino</i> Mice

Assessment of Biochemical and Histopathological Effects of Crude Venom of Cone Snail <i>Conus flavidus</i> on <i>albino</i> Mice