Characterization of the venom of the vermivorous cone snail <i>Conus fulgetrum</i>

Abdel-Wahab, Mohammed; Miyashita, Masahiro; Kitanaka, Atsushi; Juichi, Hironori; Sarhan, Moustafa; Fouda, Maged M. A.; Abdel-Rahman, Mohamed A.; Saber, Samy A.; Nakagawa, Yoshiaki

doi:10.1080/09168451.2016.1176519

Cited by 6 publications

(5 citation statements)

References 15 publications

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“…In addition, over 50% of the conopeptides detected in the venom of the studied species were smaller than 2,500 Da. [ 37 ]. Similarly, low molecular weight peptides were the most abundant in C. fulgetrum venom [ 37 ], C. marmoreus and C. bandanus venoms [ 2 ].…”

Section: Discussionmentioning

confidence: 99%

“…[ 37 ]. Similarly, low molecular weight peptides were the most abundant in C. fulgetrum venom [ 37 ], C. marmoreus and C. bandanus venoms [ 2 ]. Although these species share worm-like prey, they evolved different strategies to produce diverse conopeptides.…”

Section: Discussionmentioning

confidence: 99%

“…We reported herein that most C. taeniatus peptides were disulfide-rich, with the highest possibility of 3 disulfide bridges. Disulfide-rich peptides were also abundant in the venom of C. consors [ 43 ], C. bandanus and C. marmoreus [2] and C. fulgetrum [ 37 ]. It is well known that disulfide bonds confer conformational stability to folded proteins [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

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Proteomic analysis of Red Sea Conus taeniatus venom reveals potential biological applications

Fouda

Abdel-Wahab

Mohammadien

et al. 2021

J. Venom. Anim. Toxins incl. Trop. Dis

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Background: Diverse and unique bioactive neurotoxins known as conopeptides or conotoxins are produced by venomous marine cone snails. Currently, these small and stable molecules are of great importance as research tools and platforms for discovering new drugs and therapeutics. Therefore, the characterization of Conus venom is of great significance, especially for poorly studied species. Methods: In this study, we used bioanalytical techniques to determine the venom profile and emphasize the functional composition of conopeptides in Conus taeniatus, a neglected worm-hunting cone snail. Results: The proteomic analysis revealed that 84.0% of the venom proteins were between 500 and 4,000 Da, and 16.0% were > 4,000 Da. In C. taeniatus venom, 234 peptide fragments were identified and classified as conotoxin precursors or non-conotoxin proteins. In this process, 153 conotoxin precursors were identified and matched to 23 conotoxin precursors and hormone superfamilies. Notably, the four conotoxin superfamilies T (22.87%), O1 (17.65%), M (13.1%) and O2 (9.8%) were the most abundant peptides in C. taeniatus venom, accounting for 63.40% of the total conotoxin diversity. On the other hand, 48 non-conotoxin proteins were identified in the venom of C. taeniatus. Moreover, several possibly biologically active peptide matches were identified, and putative applications of the peptides were assigned. Conclusion: Our study showed that the composition of the C. taeniatus-derived proteome is comparable to that of other Conus species and contains an effective mix of toxins, ionic channel inhibitors and antimicrobials. Additionally, it provides a guidepost for identifying novel conopeptides from the venom of C. taeniatus and discovering conopeptides of potential pharmaceutical importance.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Proteomic analysis of Red Sea Conus taeniatus venom reveals potential biological applications

Fouda

Abdel-Wahab

Mohammadien

et al. 2021

J. Venom. Anim. Toxins incl. Trop. Dis

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“…Much less attention has been devoted to in-depth examination of insecticidal activity to develop conotoxins into biological pesticides [ 26 , 27 ]. Some vermivorous Conus species are among the largest conoideans, such as C. betulinus , which has been observed by divers to eat large polychaetes [ 2 , 28 ].…”

Section: Discussionmentioning

confidence: 99%

Screening and Validation of Highly-Efficient Insecticidal Conotoxins from a Transcriptome-Based Dataset of Chinese Tubular Cone Snail

Gao

Peng

Lin

et al. 2017

Toxins

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Most previous studies have focused on analgesic and anti-cancer activities for the conotoxins identified from piscivorous and molluscivorous cone snails, but little attention has been devoted to insecticidal activity of conotoxins from the dominant vermivorous species. As a representative vermivorous cone snail, the Chinese tubular cone snail (Conus betulinus) is the dominant Conus species inhabiting the South China Sea. We sequenced related venom transcriptomes from C. betulinus using both the next-generation sequencing and traditional Sanger sequencing technologies, and a comprehensive library of 215 conotoxin transcripts was constructed. In our current study, six conotoxins with potential insecticidal activity were screened out from our conotoxin library by homologous search with a reported positive control (alpha-conotoxin ImI from C. imperialis) as the query. Subsequently, these conotoxins were synthesized by chemical solid-phase and oxidative folding for further insecticidal activity validation, such as MTT assay, insect bioassay and homology modeling. The final results proved insecticidal activities of our achieved six conotoxins from the transcriptome-based dataset. Interestingly, two of them presented a lot of high insecticidal activity, which supports their usefulness for a trial as insecticides in field investigations. In summary, our present work provides a good example for high throughput development of biological insecticides on basis of the accumulated genomic resources.

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“…The purified conotoxins are subjected to de novo sequencing through Edman degradation [ 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 ] or MS sequencing [ 81 , 88 , 89 , 90 ] after sequential disulfide bond reduction, hydrosulphonyl alkylation, and enzymolysis, which make sequencing process much easier. PTMs are assigned with the aid of MS techniques [ 59 , 65 , 66 , 69 , 70 , 71 , 75 , 76 , 77 , 78 , 80 , 83 , 89 , 90 , 91 , 92 ]. The targeted conotoxins are then chemically synthesized through SPPS, following the subsequent oxidative...…”

Section: Conotoxins Purified From Crude Venommentioning

confidence: 99%

Discovery Methodology of Novel Conotoxins from Conus Species

Dong

et al. 2018

Marine Drugs

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Cone snail venoms provide an ideal resource for neuropharmacological tools and drug candidates discovery, which have become a research hotspot in neuroscience and new drug development. More than 1,000,000 natural peptides are produced by cone snails, but less than 0.1% of the estimated conotoxins has been characterized to date. Hence, the discovery of novel conotoxins from the huge conotoxin resources with high-throughput and sensitive methods becomes a crucial key for the conotoxin-based drug development. In this review, we introduce the discovery methodology of new conotoxins from various Conus species. It focuses on obtaining full N- to C-terminal sequences, regardless of disulfide bond connectivity through crude venom purification, conotoxin precusor gene cloning, venom duct transcriptomics, venom proteomics and multi-omic methods. The protocols, advantages, disadvantages, and developments of different approaches during the last decade are summarized and the promising prospects are discussed as well.

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Characterization of the venom of the vermivorous cone snail Conus fulgetrum

Cited by 6 publications

References 15 publications

Proteomic analysis of Red Sea Conus taeniatus venom reveals potential biological applications

Proteomic analysis of Red Sea Conus taeniatus venom reveals potential biological applications

Screening and Validation of Highly-Efficient Insecticidal Conotoxins from a Transcriptome-Based Dataset of Chinese Tubular Cone Snail

Discovery Methodology of Novel Conotoxins from Conus Species

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