Amino acid profiling in plant cell cultures: An inter‐laboratory comparison of CE‐MS and GC‐MS

Williams, Brad J.; Cameron, Carson J.; Workman, R.L.; Broeckling, Corey D.; Sumner, Lloyd W.; Smith, Joel T.

doi:10.1002/elps.200600609

Cited by 61 publications

(38 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3. The CE-MS operation has also been found to be less expensive than GC-MS since one cheap fused-silica capillary can be used in over 1500 injections [122]. Lee et al [123] used CE-MS in conjugation with computer simulation to analyze low-abundance metabolites from complex samples.…”

Section: Ce In Metabolomicsmentioning

confidence: 98%

“…Using DNA microarray technology, 2-D differential gel electrophoresis, and CE-TOF MS, this group showed the effect of perturbation at all omics levels. Williams et al [122] reported on the analysis of important amino acid profiles in different plant cells. In that report the authors concluded that CE-MS analysis could be a valid technique for profiling metabolites, and that it had advantages over GC-MS, a common analytical technique in this area, because it did not require sample derivatization, thereby making sample preparation simpler.…”

Section: Ce In Metabolomicsmentioning

confidence: 99%

See 1 more Smart Citation

CE at the omics level: Towards systems biology – An update

Song

Babar

et al. 2007

Electrophoresis

View full text Add to dashboard Cite

This review provides an updated overview of recent developments and applications of CE based on previously published reports in the field of omic research. The increased number of published articles on omics shows that the field is growing and attracting the attention of many life science researchers. Due to developments in the omics sciences, many researchers have been studying systems biology, in which biological events in organisms are systematically interpreted through the combination of complex measurements from various methods resulting in high-throughput data. Given the challenges of such complex forms of analysis, CE is a strong candidate for generating omics data useful for acquiring the qualitative and quantitative knowledge necessary for systems-level investigation. By emphasizing CE for systems biology, this review will discuss and focus on the applicability of CE to systems-based analytical data at the genomic, transcriptomic, proteomic, and metabolomic levels from 2005 to the present.

show abstract

Section: Ce In Metabolomicsmentioning

confidence: 98%

Section: Ce In Metabolomicsmentioning

confidence: 99%

CE at the omics level: Towards systems biology – An update

Song

Babar

et al. 2007

Electrophoresis

View full text Add to dashboard Cite

show abstract

“…Although the application of CE-MS to plant metabolite profiling is relatively limited (Sato et al, 2004;Takahashi et al, 2006;Williams et al, 2007), CE-MS will be used in a broader field of application due to its applicability to the polar metabolites and ease of sample preparation.…”

Section: ) Ce-msmentioning

confidence: 99%

Application of Metabolomics to Improve Tomato Fruit Productivity and Quality

Iijima

Aoki

2009

J. Japan. Soc. Hort. Sci.

View full text Add to dashboard Cite

Productivity and quality of tomato (Solanum lycopersicum) fruit largely depend on the quantity and composition of its metabolites. Recent development of mass spectrometry technology has facilitated the simultaneous detection of a large number of metabolites. This opens the possibility of investigating the mechanisms of metabolic regulation that affect fruit productivity and quality, in conjunction with genetic and omics approaches. In this article, we review recent progress in the analysis of tomato fruit metabolites based on comprehensive metabolic profiling and the identification of genes and quantitative trait loci that alter tomato fruit metabolite profiles.

show abstract

“…Nevertheless, both techniques yielded the same biological conclusions on environmentinduced changes in plant amino-acid levels. TMS derivatives with GC-MS analysis of amino acids was further compared to capillary electrophoresis coupled to MS, demonstrating that both techniques obtain similar results [34].…”

Section: Quality Control and Methods Comparisonsmentioning

confidence: 99%

Extending the breadth of metabolite profiling by gas chromatography coupled to mass spectrometry

Fiehn

2008

TrAC Trends in Analytical Chemistry

208

148

View full text Add to dashboard Cite

Gas chromatography coupled to mass spectrometry (GC-MS) is one of the most frequently used tools for profiling primary metabolites. Instruments are mature enough to run large sequences of samples; novel advancements increase the breadth of compounds that can be analyzed, and improved algorithms and databases are employed to capture and utilize biologically relevant information. Around half the published reports on metabolite profiling by GC-MS focus on biological problems rather than on methodological advances. Applications span from comprehensive analysis of volatiles to assessment of metabolic fluxes for bioengineering. Method improvements emphasize extraction procedures, evaluations of quality control of GC-MS in comparison to other techniques and approaches to data processing. Two major challenges remain: rapid annotation of unknown peaks; and, integration of biological background knowledge aiding data interpretation.

show abstract

Amino acid profiling in plant cell cultures: An inter‐laboratory comparison of CE‐MS and GC‐MS

Cited by 61 publications

References 33 publications

CE at the omics level: Towards systems biology – An update

CE at the omics level: Towards systems biology – An update

Application of Metabolomics to Improve Tomato Fruit Productivity and Quality

Extending the breadth of metabolite profiling by gas chromatography coupled to mass spectrometry

Contact Info

Product

Resources

About