Amino Acid Correction of Regulatory Volume Decrease Evoked by Hypotonic Stress in Mouse Oocytes In Vitro

Abstract

Regulatory volume decrease in response to hypotonic stress is typical of the oocytes and early mouse embryos. Changes in the kinetics of osmotic reaction can be used as a marker of the modulating effect of the incubation medium on transmembrane transport in embryonic cells. Quantitative laser scanning microtomography (QLSM) was used to measure oocyte volume. In this paper, it is shown that addition of 5 μM glycine, taurine, or GABA, as well as ATP to Dulbecco's medium abolished the regulatory volume decrease in mature mouse oocytes.

Activation of volume-sensitive Cl− channel mediates autophagy-related cell death in myocardial ischaemia/reperfusion injury

“…Definite evidence determines that activation of VSOR Cl − channel is concerned with cardiac contractile dysfunction [22, 24, 25], however DCPIB may attenuate cardiomyocyte apoptosis and rescue cardiac function by inhibition of VSOR Cl − channel-activated [26–29]. The present study proposes a novel evidence that VSOR Cl − channel-activated by ROS may cause autophagic cell shrinkage, leading to intracellular acidification and autophagy-related cell death.…”

“…Cell death such as apoptotic and necrotic cell death are associated with alterations of cell volume regulation, the control of cell volume is essential not only for maintaining physiological cell activities but also for cell survival. The RVD may be caused by activation of VSOR Cl − channel in many mammalian cell [22, 24, 30]. Moreover, the RVD is abolished when a blocker of VSOR Cl − channel DCPIB is added [30].…”

“…Cell volume changes have been used as one of the key discriminators between apoptosis and necrosis, which are associated with persistent whole-cell shrinkage and swelling, respectively. The regulatory volume decrease (RVD) observed soon after cell swelling is accomplished by parallel activation of volume-sensitive outwardly rectifying (VSOR) Cl − channel in numerous cell types [22–24]. On the other hand, non-swelling-coupled activation of VSOR Cl − channel has been reported to cause apoptosis volume decrease (AVD) in many cells [25–28].…”

Differences in resistance against osmotic challenge among C57BL/6, DBA/2 and their hybrid mice metaphase II (MII) stage oocytes

“…Cells are exposed to various osmotic changes in vivo. A previous report has shown that cell volume increases because of water flow into the cell in a hypotonic environment (Pogorelova et al, 2015). Although cells are forced to change their volume according to extracellular environments with varying osmolalities, cells have a system to resist volume variations and maintain a stable volume.…”

Gambogenic Acid Triggers Apoptosis of Human Nasopharyngeal Carcinoma CNE-2Z Cells through Activating Volume-Sensitive Outwardly Rectifying Chloride Channels

“…The VSOR Cl -channel is the key to the occurrence of apoptosis by inducing apoptotic volume decrease (AVD), a major hallmark of cell apoptosis and an early prerequisite to apoptotic events. Observed soon after cell swelling, the regulatory volume decrease (RVD) is completed by parallel activation of the VSOR Cl -channels in numerous cell types [13,14]. In addition, non-swelling-coupled activation of the VSOR Cl -channels is believed to cause AVD in many cells [15,16].…”