Amino acid concentration in the interstitium of human skeletal muscle: a microdialysis study

Gutiérrez, Alberto; Anderstam, Björn; Alvestrand, Anders

doi:10.1046/j.1365-2362.1999.00551.x

Cited by 33 publications

(29 citation statements)

References 34 publications

(41 reference statements)

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“…To investigate if rupture of the sarcolemma occurs when the microdialysis probes are inserted in human skeletal muscle, the dialysate was analyzed for carnosine concentrations. Carnosine is found in high concentrations inside the muscle cell (12) but is undetectable in blood plasma (15). High carnosine concentrations were observed in the dialysate during the first 20-W exercise period, indicating that rupture of the sarcolemma did occur.…”

Section: Discussionmentioning

confidence: 97%

Muscle interstitial potassium kinetics during intense exhaustive exercise: effect of previous arm exercise

Nordsborg

Mohr²,

Pedersen³

et al. 2003

American Journal of Physiology-Regulatory, Integrative and Comp

127

142

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Interstitial K+ ([K+]i) was measured in human skeletal muscle by microdialysis during exhaustive leg exercise, with (AL) and without (L) previous intense arm exercise. In addition, the reproducibility of the [K+]i determinations was examined. Possible microdialysis-induced rupture of the sarcolemma was assessed by measurement of carnosine in the dialysate, because carnosine is only expected to be found intracellularly. Changes in [K+]i could be reproduced, when exhaustive leg exercise was performed on two different days, with a between-day difference of ∼0.5 mM at rest and 1.5 mM at exhaustion. The time to exhaustion was shorter in AL than in L (2.7 ± 0.3 vs. 4.0 ± 0.3 min; P < 0.05). Furthermore, [K+]i was higher from 0 to 1.5 min of the intense leg exercise period in AL compared with L (9.2 ± 0.7 vs. 6.4 ± 0.9 mM; P < 0.001) and at exhaustion (11.9 ± 0.5 vs. 10.3 ± 0.6 mM; P < 0.05). The dialysate content of carnosine was elevated by exercise, but low-intensity exercise resulted in higher dialysate carnosine concentrations than subsequent intense exercise. Furthermore, no relationship was found between carnosine concentrations and [K+]i. Thus the present data suggest that microdialysis can be used to determine muscle [K+]i kinetics during intense exercise, when low-intensity exercise is performed before the intense exercise. The high [K+]i levels reached at exhaustion can be expected to cause fatigue, which is supported by the finding that a faster accumulation of interstitial K+, induced by prior arm exercise, was associated with a reduced time to fatigue.

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Section: Discussionmentioning

confidence: 97%

Muscle interstitial potassium kinetics during intense exhaustive exercise: effect of previous arm exercise

Nordsborg

Mohr²,

Pedersen³

et al. 2003

American Journal of Physiology-Regulatory, Integrative and Comp

127

142

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“…Also in humans, carnosine concentration markedly increases in the skeletal muscle interstitium during leg extension exercise at 20W, as determined by microdialysis [21]. Although some researchers believe that the contraction-induced release of carnosine by muscles is a measure of muscle damage and exertional rhabdomyolysis [21][22][23], Nagai et al [20] have proposed that carnosine release from muscle is a regulated process. The fact that proton accumulation (acidosis) can stimulate the proton-driven dipeptide transporter (PEPT2) provides one possible mechanism as to why muscles would release/secrete more carnosine during contractions than at rest.…”

Section: Proposed Role Of Skeletal Muscle In Whole-body Carnosine Metmentioning

confidence: 99%

“…The fact that proton accumulation (acidosis) can stimulate the proton-driven dipeptide transporter (PEPT2) provides one possible mechanism as to why muscles would release/secrete more carnosine during contractions than at rest. The observation that the histidine concentration increases in parallel with carnosine in human muscle interstitium [23] suggests that at least a portion of the contraction-induced release of carnosine from muscle is immediately hydrolyzed by the serum carnosinase present in the interstitium/circulation in humans. In vitro animal experiments have suggested that the muscle carnosine concentration decreases following a period of contractions [24], further supporting the hypothesis of contraction-induced carnosine release.…”

Section: Proposed Role Of Skeletal Muscle In Whole-body Carnosine Metmentioning

confidence: 99%

Muscle Carnosine Metabolism and β-Alanine Supplementation in Relation to Exercise and Training

et al. 2010

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“…The procedure has reached widespread application in neurosurgery 12,13 and the neurointensive care unit, 14-16 cardiovascular 17 and plastic surgery. 18,19 Furthermore, it allows monitoring metabolism in skeletal muscle, 20,21 adipose tissue, [22][23][24] and tissue glucose in diabetes mellitus diseases. 25 It also has been used to monitor liver grafts in rats.…”

mentioning

confidence: 99%

Metabolic changes in the liver graft monitored continuously with microdialysis during liver transplantation in a pig model

Nowak

Ungerstedt

Wernerman

et al. 2002

Liver Transplantation

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Microdialysis provides the opportunity to continuously monitor metabolic changes in tissue. The aim of the study is to monitor metabolic changes in the liver graft over time during transplantation in a pig model. Fourteen littermate female pigs with a body weight of 30 to 34 kg were used for seven orthotopic liver transplantations. Intrahepatic implantation of a microdialysis catheter into the liver graft was performed in the donor. Microdialysis samples were collected at 20-minute intervals during the donor operation, cold preservation, and for 7 hours after reperfusion in the recipient. Glucose, lactate, pyruvate, and glycerol concentrations were measured. After cold perfusion, glucose, lactate, and glycerol levels increased, whereas pyruvate levels decreased rapidly. During cold storage, glucose and glycerol levels increased, whereas lactate levels remained stable and pyruvate levels were undetectable. During implantation of the liver graft, glucose, lactate, and glycerol levels showed an accelerated increase. After portal reperfusion, glucose, lactate, and glycerol levels continued to increase for another 40 to 60 minutes, after which they decreased and finally settled at normal levels. At this time, pyruvate levels increased, with a peak within 2 hours after reperfusion, and then decreased to normal levels. Calculated lactate-pyruvate ratio increased after cold perfusion and remained stable during cold storage. During rewarming, it showed an accelerated increase, but after reperfusion, it decreased rapidly. Rewarming and reperfusion are most harmful to the liver, reflected by an accelerated increase in glucose and glycerol levels and lactate-pyruvate ratio. High intrahepatic glucose levels during ischemia appear to be a liver-specific event, which may represent glycogen degradation in injured hepatocytes. (Liver Transpl 2002;8:424-432.)

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Amino acid concentration in the interstitium of human skeletal muscle: a microdialysis study

Cited by 33 publications

References 34 publications

Muscle interstitial potassium kinetics during intense exhaustive exercise: effect of previous arm exercise

Muscle interstitial potassium kinetics during intense exhaustive exercise: effect of previous arm exercise

Muscle Carnosine Metabolism and β-Alanine Supplementation in Relation to Exercise and Training

Metabolic changes in the liver graft monitored continuously with microdialysis during liver transplantation in a pig model

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