Amino acid-based hydrophobic affinity cryogel for protein purification from ora-pro-nobis (Pereskia aculeata Miller) leaves

Neves, Isabelle Cristina Oliveira; Rodrigues, Adrise Aparecida; Valentim, Thamires Teixeira; Meira, Ana Cristina Freitas de Oliveira; Silva, Sérgio Henrique; Veríssimo, Lizzy Ayra Alcântara; Resende, Jaime Vilela de

doi:10.1016/j.jchromb.2020.122435

Cited by 10 publications

(3 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The swelling capacity was characterized as the amount of water absorbed by the dry cryogel and the degree of expansion by the difference between the mass of the dehydrated and hydrated cryogel, presenting the values of 11.4 ± 0.5 g/g and 16.26 ± 0.3 mL/g, respectively. These analyses show that the cryogel mass occupies a large volume when hydrated, reinforcing the nature of its porous structure, as well as a sponge and elastic characteristic and consistent, confirming what has been reported for other types of activated cryogels [29‐30, 21, 31].…”

Section: Resultssupporting

confidence: 86%

“…These low and constant values along flow velocities indicate that the mass transfer region is small, and the mass transfer occur predominantly by convection. This behavior is like the studies reported by Mól et al [21] and Neves et al [30]. The experimental pressure drop as a function of different flow velocities are shown in Figure 6.…”

Section: Characterization Of the Affinity Columnsupporting

confidence: 88%

See 1 more Smart Citation

Synthesis and characterization of supermacroporous cryogel with immobilized p‐aminobenzenesulfonamide as affinity ligand for the purification of lactoperoxidase from whey

Maciel

Mól

Veríssimo

et al. 2022

J of Separation Science

View full text Add to dashboard Cite

This study proposed the development of a monolithic supermacroporous affinity column for direct capture of lactoperoxidase, a glycoprotein present in milk, whey, and colostrum, with several applications due to its wide antimicrobial activity. A poly(acrylamide)-based cryogel was produced by radical co-polymerization of monomers in frozen aqueous solution and activated with p-aminobenzenesulfonamide as a ligand for specific interaction with the lactoperoxidase. The axial liquid dispersion coefficients at different liquid flow rates were determined by measuring residence time distributions using the tracer pulse-response method. The axial dispersion coefficient was low and the height equivalent to theoretical plate was not dependent on the flow velocity. The adsorptive capacity of affinity cryogel was studied as a function of flow velocity and the best condition was 0.9 cm/min. The response surface methodology was applied to optimize the capture of the enzyme, as a function of pH and salt concentration. Higher purification factor value was found at a salt concentration of 80 mmol/L and pH of 8.0 (p < 0.05). There was no influence of the variables under study on the yield (p > 0.05). The results indicated that affinity cryogel is a promising chromatography support for the use in high-throughput one-step purification of lactoperoxidase from whey.

show abstract

Section: Resultssupporting

confidence: 86%

Section: Characterization Of the Affinity Columnsupporting

confidence: 88%

Synthesis and characterization of supermacroporous cryogel with immobilized p‐aminobenzenesulfonamide as affinity ligand for the purification of lactoperoxidase from whey

Maciel

Mól

Veríssimo

et al. 2022

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…At the same time, the high-pressure and enzymatic hydrolysis treatments may also increase hydrophobic amino acid content in gelatin peptides, thereby improving their stability and antioxidant properties. Studies have also indicated that proteins with a high content of hydrophobic amino acids exhibit greater stability ( Cristina Oliveira Neves, Aparecida Rodrigues, Teixeira Valentim, Cristina Freitas de Oliveira Meira, Henrique Silva, Ayra Alcântara Veríssimo, et al, 2020 ).…”

Section: Resultsmentioning

confidence: 99%

Cowhide gelatin peptide as a source of antioxidants for inhibiting the deterioration of pudding quality during storage

Wang,

Zhao,

et al. 2024

Food Chemistry: X

View full text Add to dashboard Cite

Performance of pseudo‐specific cryogel in lysozyme purification from chicken egg white

et al. 2022

View full text Add to dashboard Cite

The application of cryogels for biomolecule purification has expanded due to their adsorption efficiency and operational advantages. In this study, polyacrylamide cryogels functionalized with L-phenylalanine (cryogel-Phe) via the glutaraldehyde method were designed for lysozyme adsorption. Cryogel functionalization was confirmed by Fourier-transform infrared spectroscopy and Kjeldahl analysis, indicating the immobilization of 458.65 mg phenylalanine g cryogel À1 . Cryogel-Phe showed high porosity (0.95) and a Young's modulus of 526.71 kPa. Thermogravimetric analysis indicated that thermal degradation occurred above 200 C. Differential scanning calorimetry and X-ray diffraction confirmed that the cryogel material was amorphous. In addition, the column presented a hydraulic permeability of 4.15 Â 10 À13 m 2 , axial dispersion ranging from 10 À7 to 10 À6 m 2 s À1 , and a height equivalent to a theoretical plate ranging from 0.10 to 0.21 cm. The highest adsorption of lysozyme (67.65 mg g À1 ) was obtained using sodium thiocyanate saline solution (0.025 mol L À1 , pH 5.0). The ability of the cryogel-Phe column to capture and purify lysozyme was confirmed by high enzymatic activity (1294.17 U ml À1 ), purity (87.92%), purification factor (11.49), and sulphate-polyacrylamide electrophoresis gel (SDS-PAGE) electrophoresis gel.

show abstract

Amino acid-based hydrophobic affinity cryogel for protein purification from ora-pro-nobis (Pereskia aculeata Miller) leaves

Cited by 10 publications

References 69 publications

Synthesis and characterization of supermacroporous cryogel with immobilized p‐aminobenzenesulfonamide as affinity ligand for the purification of lactoperoxidase from whey

Synthesis and characterization of supermacroporous cryogel with immobilized p‐aminobenzenesulfonamide as affinity ligand for the purification of lactoperoxidase from whey

Cowhide gelatin peptide as a source of antioxidants for inhibiting the deterioration of pudding quality during storage

Performance of pseudo‐specific cryogel in lysozyme purification from chicken egg white

Contact Info

Product

Resources

About