Amino acid analysis by reversed-phase high-performance liquid chromatography

Iwaki, Kazuo; Nimura, Noriyuki; Hiraga, Yukio; Kinoshita, Toshio; Takeda, Kazuyoshi; Ogura, Haruo

doi:10.1016/s0021-9673(01)92624-x

Cited by 67 publications

(19 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The N content of feed was determined as Kjedahl N 3 6.25 using the LECO FP 528 instrument (Leco Instruments, UK Ltd, Stockport, Cheshire, UK). The dietary concentrations of lysine, threonine, tryptophan, methionine and cysteine were determined by high-performance liquid chromatography (HPLC) (Iwaki et al, 1987). The N content of fresh faeces was analysed by the macro-Kjeldahl technique using a Buchi distillation apparatus.…”

Section: Microbiologymentioning

confidence: 99%

Effect of crude protein concentration and sugar-beet pulp on nutrient digestibility, nitrogen excretion, intestinal fermentation and manure ammonia and odour emissions from finisher pigs

Lynch

O’Shea

Sweeney

et al. 2008

Animal

View full text Add to dashboard Cite

A 2 3 2 factorial experiment was conducted to investigate the interaction between high and low dietary crude protein (CP) (200 v. 150 g/kg) and sugar-beet pulp (SBP) (200 v. 0 g/kg) on nutrient digestibility, nitrogen (N) excretion, intestinal fermentation and manure ammonia and odour emissions from 24 boars (n 5 6, 74.0 kg live weight). The diets were formulated to contain similar concentrations of digestible energy (13.6 MJ/kg) and lysine (10.0 g/kg). Pigs offered SBP-containing diets had a reduced (P , 0.05) digestibility of dry matter, ash, N, gross energy and an increased ( P , 0.001) digestibility of neutraldetergent fibre compared with pigs offered diets containing no SBP. There was an interaction between CP and SBP on urinary N excretion and the urine : faeces N ratio. Pigs offered the 200 g/kg CP SBP-based diet had reduced urine : faeces N ratio (P , 0.05) and urinary N excretion ( P , 0.05) compared with those offered the 200 g/kg CP diet without SBP. However, there was no effect of SBP in pigs offered 150 g/kg CP diets. Manure ammonia emissions were reduced by 33% from 0 to 240 h (P , 0.01); however, odour emissions were increased by 41% (P , 0.05) when pigs were offered SBP diets. Decreasing dietary CP to 150 g/kg reduced total N excretion ( P , 0.001) and ammonia emissions from 0 to 240 h ( P , 0.05). There was an interaction between dietary CP and SBP on branched-chain fatty acids ( P , 0.001) in caecal digesta. Pigs offered the 200 g/kg CP SBP-containing diet reduced branched-chain fatty acids in the caecum compared with pigs offered the 200 g/kg CP diet containing no SBP. However, there was no effect of SBP in the 150 g/kg CP diet. In conclusion, pigs offered SBP-containing diets had a reduced manure ammonia emissions and increased odour emissions compared with diets containing no SBP. Pigs offered the 200 g/kg CP SBP-containing diet had a reduced urine : faeces N ratio and urinary N excretion compared with those offered the 200 g/kg CP diet containing no SBP.

show abstract

Section: Microbiologymentioning

confidence: 99%

Effect of crude protein concentration and sugar-beet pulp on nutrient digestibility, nitrogen excretion, intestinal fermentation and manure ammonia and odour emissions from finisher pigs

Lynch

O’Shea

Sweeney

et al. 2008

Animal

View full text Add to dashboard Cite

show abstract

“…The N content of feed and urine was determined using the LECO FP 528 instrument (Leco Instruments (UK) Ltd). The dietary concentrations of lysine, threonine, tryptophan, methionine and cysteine were determined by high-performance liquid chromatography (Iwaki et al, 1987). The N content of fresh faeces was analysed by the Lynch, Sweeny, Callan, Flynn and O'Doherty macro-Kjeldahl technique using a Buchi digestion and distillation apparatus.…”

Section: Microbiologymentioning

confidence: 99%

The effect of high and low dietary crude protein and inulin supplementation on nutrient digestibility, nitrogen excretion, intestinal microflora and manure ammonia emissions from finisher pigs

Lynch

Sweeney

Callan

et al. 2007

Animal

View full text Add to dashboard Cite

A 2 3 2 factorial experiment was performed to investigate the interaction between a high-and low-crude-protein (CP) diet (200 v. 140 g/kg) and inulin supplementation (0 v. 12.5 g/kg) on nutrient digestibility, nitrogen (N) excretion, intestinal microflora, volatile fatty acid (VFA) concentration and manure ammonia emissions from 24 boars (n 5 6, 74.0 kg live weight). The diets were formulated to contain similar concentrations of digestible energy and lysine. Pigs offered the high-CP diets had a higher excretion of urinary N (P , 0.001), faecal N (P , 0.01) and total N (P , 0.001) than the pigs offered the low-CP diets. Inulin supplementation increased faecal N excretion (P , 0.05) and decreased the urine N : faeces N ratio (P , 0.05) compared with the inulin-free diets. There was no effect (P . 0.05) of dietary treatment on N retention. There was an interaction (P , 0.05) between dietary CP concentration and inulin supplementation on caecal Enterobacteria spp. Pigs offered the diet containing 200 g/kg of CP plus inulin decreased the population of Enterobacteria spp. compared to those with the inulinsupplemented 140 g/kg CP diet. However, CP level had no significant effect on the population of Enterobacteria spp. in the unsupplemented diets. Inulin supplementation increased caecal Bifidobacteria (P , 0.01) compared with the inulin-free diets. There was no effect of inulin supplementation on VFA concentration or intestinal pH (P . 0.05). Pigs offered the 200 g/kg CP diets had higher (P , 0.05) manure ammonia emissions from 0 to 240 h of storage than pigs offered the 140 g/kg CP. In conclusion, inulin supplementation resulted in an increase in Bifidobacteria concentration and a reduction in Enterobacteria spp. at the high CP level indicating that inulin has the ability to beneficially manipulate gut microflora in a proteolytic environment.

show abstract

“…An RP-HPLC method, based on the formation of fluorescing naphthylcarbamyl derivatives by reaction with succinimidonaphthylcarbamate (SINC), has been developed to make precolumn derivatization of AAs easier. The derivatives are eluted with an aqueous solution of sodium acetate of pH 6.3 containing ACN and fluorescence detection is used [20]. The method involving derivatization of AAs with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AccQ fluorescence reagent) is highly sensitive and its applications to binder analysis are described in refs.…”

Section: Introductionmentioning

confidence: 99%

An evaluation of GC‐MS and HPLC‐FD methods for analysis of protein binders in paintings

Přikryl

Havlíčková

Pacáková³

et al. 2006

J of Separation Science

View full text Add to dashboard Cite

Two chromatographic methods have been compared for analysis of protein-binding media used in paintings, namely, HPLC with fluorescence detection and GC-MS. The proteins were hydrolyzed to the corresponding amino acids (AAs) by gaseous HCl and the AAs were derivatized with methyl chloroformate, followed by GC-MS or by HPLC after derivatization with the AccQ fluorescence reagent. The hydrolysis, derivatization reactions and the chromatographic procedures have been optimized and applied to standard binding media, model and real samples of paintings. The methods have been compared and critically evaluated.

show abstract

Amino acid analysis by reversed-phase high-performance liquid chromatography

Cited by 67 publications

References 9 publications

Effect of crude protein concentration and sugar-beet pulp on nutrient digestibility, nitrogen excretion, intestinal fermentation and manure ammonia and odour emissions from finisher pigs

Effect of crude protein concentration and sugar-beet pulp on nutrient digestibility, nitrogen excretion, intestinal fermentation and manure ammonia and odour emissions from finisher pigs

The effect of high and low dietary crude protein and inulin supplementation on nutrient digestibility, nitrogen excretion, intestinal microflora and manure ammonia emissions from finisher pigs

An evaluation of GC‐MS and HPLC‐FD methods for analysis of protein binders in paintings

Contact Info

Product

Resources

About