Amino acid analyses of the exosome-eluted fractions from human serum by HPLC with fluorescence detection

Onozato, Mayu; Tanaka, Yuriko; Arita, Michitsune; Sakamoto, Tatsuya; Ichiba, Hideaki; Sadamoto, Kiyomi; Kondo, Motonari; Fukushima, Takeshi

doi:10.1016/j.plabm.2018.e00099

Cited by 13 publications

(12 citation statements)

References 15 publications

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“…When the amount of the D‐Ser was compared with the amount of L‐Ser, the L‐Ser concentrations were higher than that of the D‐Ser. The above quantitative results are basically consistent with those already reported . In conclusion, we could use the OTPTHE derivatization reagent to separate and detect a pair of DL‐amino acid enantiomers.…”

Section: Resultssupporting

confidence: 92%

“…The above quantitative results are basically consistent with those already reported. 6,[41][42][43] In conclusion, we could use the OTPTHE derivatization reagent to separate and detect a pair of DL-amino acid enantiomers. These results proved that OTPTHE could be used as a kind of effective derivatization reagent to separate and detect a pair of DL-amino acid enantiomers in real biosamples.…”

Section: Determination Of Dl-ser Enantiomers In Human Plasmamentioning

confidence: 93%

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Evaluation of chiral separation efficiency of a novel OTPTHE derivatization reagent: Applications to liquid‐chromatographic determination of DL‐serine in human plasma

Han

Jin

et al. 2019

Chirality

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A novel chiral derivatization reagent, the N‐[1‐oxo‐5‐(triphenylphosphonium)pentyl]‐ (R)‐1,3‐thiazolidinyl‐4‐N‐hydroxysuccinimide ester bromide salt (OTPTHE), was developed for the separation and selective detection of chiral DL‐amino acids by RP‐HPLC analysis. The OTPTHE reacted with DL‐amino acids at 60°C maintained for 30 minutes in the presence of 100 mM borate buffer (pH 9.5). The separability of the diastereomeric derivatives was evaluated in terms of the resolution value (Rs) using 13 kinds of DL‐amino acids, which were completely separated by reversed‐phase chromatography using C18 column at 254 nm. The Rs of the DL‐amino acids varied from 1.62 to 2.51. As for the application of the DL‐amino acids, the determination of DL‐Ser in the human plasma of healthy volunteers was performed based on our developed method. It was shown that linear calibrations were available with high coefficients of correlation (r2 > 0.9997). The limit of detection (S/N = 3) of the DL‐Ser enantiomers was 5.0 pmol; the relative standard deviations of the intraday and interday variations were below 4.56%; the accuracy ranged between 95.40%‐110.06% and 95.45%‐109.80%, respectively; the mean recoveries (%) of the DL‐Ser spiked in the human plasma were 99.49%‐103.74%. The amounts of DL‐Ser in the human plasma of healthy volunteers were determined.

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Section: Resultssupporting

confidence: 92%

Section: Determination Of Dl-ser Enantiomers In Human Plasmamentioning

confidence: 93%

Evaluation of chiral separation efficiency of a novel OTPTHE derivatization reagent: Applications to liquid‐chromatographic determination of DL‐serine in human plasma

Han

Jin

et al. 2019

Chirality

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“…Characteristic peaks of “pure” PTPS components and their likely assignments reported previously [ 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 ] are listed in Supplementary Materials Table S1 . For example, UC-ENV-specific peak at 1004 cm −1 may reflect the presence of an essential α-amino acid phenylalanine [ 24 ], however enrichment of this compound in plasma ENV compared to total plasma was not detected [ 36 ]. The composition of tested solution included various plasma components and was too complex for precise assigning.…”

Section: Resultsmentioning

confidence: 99%

Formation and Evaluation of a Two-Phase Polymer System in Human Plasma as a Method for Extracellular Nanovesicle Isolation

et al. 2021

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The aim of the study was to explore the polyethylene glycol–dextran two-phase polymer system formed in human plasma to isolate the exosome-enriched fraction of plasma extracellular nanovesicles (ENVs). Systematic analysis was performed to determine the optimal combination of the polymer mixture parameters (molecular mass and concentration) that resulted in phase separation. The separated phases were analyzed by nanoparticle tracking analysis and Raman spectroscopy. The isolated vesicles were characterized by atomic force microscopy and dot blotting. In conclusion, the protein and microRNA contents of the isolated ENVs were assayed by flow cytometry and by reverse transcription followed by quantitative polymerase chain reaction (RT-qPCR), respectively. The presented results revealed the applicability of a new method for plasma ENV isolation and further analysis with a diagnostic purpose.

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“…It is probable that these factors are transferred at least in part by EVs. D-serine, for example, was evidenced by high performance liquid chromatography (HPLC) in exosomes purified from human serum [198]. Similarly, astrocytic EVs contain functional excitatory amino acid transporters (EAAT)-1 and -2, fundamental for glutamate homeostasis, together with Na + /K + -dependent ATPase and glutamine synthetase (GS) [197] ( Figure 6).…”

Section: Synaptic Plasticity: the Possible Role Of Evs And Their Cargoesmentioning

confidence: 99%

Cell-to-Cell Communication in Learning and Memory: From Neuro- and Glio-Transmission to Information Exchange Mediated by Extracellular Vesicles

Schiera

Liegro

2019

IJMS

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Most aspects of nervous system development and function rely on the continuous crosstalk between neurons and the variegated universe of non-neuronal cells surrounding them. The most extraordinary property of this cellular community is its ability to undergo adaptive modifications in response to environmental cues originating from inside or outside the body. Such ability, known as neuronal plasticity, allows long-lasting modifications of the strength, composition and efficacy of the connections between neurons, which constitutes the biochemical base for learning and memory. Nerve cells communicate with each other through both wiring (synaptic) and volume transmission of signals. It is by now clear that glial cells, and in particular astrocytes, also play critical roles in both modes by releasing different kinds of molecules (e.g., D-serine secreted by astrocytes). On the other hand, neurons produce factors that can regulate the activity of glial cells, including their ability to release regulatory molecules. In the last fifteen years it has been demonstrated that both neurons and glial cells release extracellular vesicles (EVs) of different kinds, both in physiologic and pathological conditions. Here we discuss the possible involvement of EVs in the events underlying learning and memory, in both physiologic and pathological conditions.

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Amino acid analyses of the exosome-eluted fractions from human serum by HPLC with fluorescence detection

Cited by 13 publications

References 15 publications

Evaluation of chiral separation efficiency of a novel OTPTHE derivatization reagent: Applications to liquid‐chromatographic determination of DL‐serine in human plasma

Evaluation of chiral separation efficiency of a novel OTPTHE derivatization reagent: Applications to liquid‐chromatographic determination of DL‐serine in human plasma

Formation and Evaluation of a Two-Phase Polymer System in Human Plasma as a Method for Extracellular Nanovesicle Isolation

Cell-to-Cell Communication in Learning and Memory: From Neuro- and Glio-Transmission to Information Exchange Mediated by Extracellular Vesicles

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