Amino acid activation of glyceraldehyde-3-phosphate dehydrogenase from chlorella

The Regulation of Carbohydrate Metabolism

“…The heptose phosphates may inhibit glyceraldehyde-3-P formation causing accumulation of 1,3-Prglycerate and 3-P glycerate which may in turn inhibit ribulose 5-phosphate kinase and ribulose 1,5-diphosphate carboxylase and thus provide a mechanism for control of the Calvin cycle (143). Cysteine and other amino acids such as glycine and alanine are reported to activate the NAD-and NADP-glyceraldehyde 3-phosphate dehydrogenases from Chlorella pyrenoidosa, Scenedesmus aculus, and spinach leaves (154). Duggleby & Dennis (49) purified the NAD-glyceraldehyde 3-phosphate dehydrogenase of pea seeds.…”

Allosteric regulation of NAD(NADP)‐dependent glyceraldehyde‐3‐phosphate dehydrogenase from Chlorella by α‐amino acids, dithiothreitol and ATP

“…Thus, chloroplast GAPD is shown to be activated by light [l-3], as well as by some metabolites such as NADPH [4], ATP [5,6], 1,3-diphosphoglycerate [7]. Previous experiments in our laboratory indicate that a-amino acids (but not p-and y-amino acids) also activate the enzyme in cell-free extracts from Chlorella [B], Scenedesmus and spinach leaves [9]. The activating effect of the amino acids has been proved with pig * To whom correspondence should be addressed Abbreviations: GAPD, D-glyceraldehyde-3-phosphate dehydrogenase;…”