Amine‐malonaldehyde condensation products and their relative color contribution in the thiobarbituric acid test

Buttkus, H.; Bose, R. J.

doi:10.1007/bf02582530

Cited by 57 publications

(26 citation statements)

References 16 publications

(21 reference statements)

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“…Analysis was achieved using a 5 m Hyperclone C18 column (150 × 4.6 mm) and a gradient elution program, as summarized in supplementary Table I. A constant fl ow rate of 1 ml/min was employed, and the eluent was monitored at 532 nm ( 10 ) by DAD; spectral scans were collected over the range of 500 to 580 nm. The fl uorescence detector used an excitation wavelength of 515 nm and an emission wavelength of 553 nm ( 8 ). An Agilent Technologies 1200 series LC coupled to an Agilent 6130 Quadrupole LC-MS was utilized with a 5 m HyperClone C18 analytical column (150 × 4.6 mm) maintained at 40°C, and a fl ow rate of 1 ml/min.…”

Section: Hplc and Lc/ms Analysismentioning

confidence: 99%

A specific, accurate, and sensitive measure of total plasma malondialdehyde by HPLC

Moselhy

Reid

Yousef

et al. 2013

Journal of Lipid Research

141

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The process of lipid peroxidation results in a range of intermediates and end products including lipid hydroperoxides, aldyehydes, and malondialdehyde (MDA). These aldehydes and lipid hydroperoxides form DNA adducts and may result in extensive single-strand and doublestrand breaks ( 4 ). Various intermediates and end products generated during the lipid peroxidation cascade have been assayed, but the most-commonly employed approach continues to be the thiobarbituric acid (TBA) test ( 5 ).Although it is widely acknowledged that TBA reacts with a range of oxidized lipids, both saturated and unsaturated aldehydes, ( 6, 7 ) sucrose, and urea ( 8 ), to form various chromogens, referred to as TBA-reactive substances (TBARS), it is the reaction of TBA with MDA to produce a pink pigment ( 9 ) with an absorption maximum at 532 nm ( 10 ) and mass ion at 323 amu ( 11 ) that is a true indicator of lipid peroxidation. Historically, the TBARS test has been assayed by ultraviolet (UV) spectrophotometry or fl uorescence assays, but the specifi city of the TBA test, enabling the selective determination of MDA, may be achieved via chromatographic separation of the pink TBA 2 -MDA adduct ( 12 ). The adoption of HPLC techniques improves assay specifi city and the sensitivity of MDA determination, and we have previously confi rmed the validity of quantifying the TBA 2 -MDA adduct as a specifi c measure of lipid peroxidation in human biological fl uids ( 13 ).Despite the widely acknowledged limitations of the TBARS test ( 14 ), and in particular its lack of specifi city, it continues to be reported as a true measure of MDA in clinical disease ( 1,15,16 ). It is proposed that the poor assay specifi city associated with TBARS assays may lead to an overestimation of the levels of MDA in human plasma and other biological tissues and fl uids, and this, in turn, may Abstract Malondialdehyde (MDA) is one of the most commonly reported biomarkers of lipid peroxidation in clinical studies. The reaction of thiobarbituric acid (TBA) with MDA to yield a pink chromogen attributable to an MDA-TBA 2 adduct is a common assay approach with products being quantified by ultraviolet-Vis assay as nonspecific TBAreactive substances (TBARS) or chromatographically as MDA. The specifi city of the TBARS assay was compared with both chromatographic assays for total plasma MDA. The levels of total plasma MDA were signifi cantly lower than the plasma TBARS in each of the samples examined, and interestingly, the interindividual variation apparent in the level of plasma MDA was not evident in the plasma TBARS assay. Each of the four online chromatographic detectors yielded a precise, sensitive, and accurate determination of total plasma MDA, and selected-ion monitoring was the most-accurate assay (101.3%, n = 4). The online diode array detectors provided good assay specifi city (peak purity index of 999), sensitivity, precision, and accuracy. This research demonstrates the inaccuracy that is inherent in plasma TBARS assays, which claim to quantify MDA, and it is pro...

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Section: Hplc and Lc/ms Analysismentioning

confidence: 99%

A specific, accurate, and sensitive measure of total plasma malondialdehyde by HPLC

Moselhy

Reid

Yousef

et al. 2013

Journal of Lipid Research

141

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“…Malondialdehyde (MDA) determination was performed as described (Buttkus and Rose, 1972), using crude liver homogenates. Commercially prepared MDA was used as the standard.…”

Section: Lipid Peroxidationmentioning

confidence: 99%

Lysosomal Leakage and Lack of Adaptation of Hepatoprotective Enzyme Contribute to Enhanced Susceptibility to Ethanol‐Induced Liver Injury in Female Rats

Donohue

Curry-McCoy

Nanji

et al. 2007

Alcoholism Clin & Exp Res

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Thus, female rats given ethanol in a diet containing fish oil exhibited more severe liver damage than males. We propose that this difference results, in part, from a greater tendency by females to accumulate hepatic fat, thereby enhancing the potential for oxidative stress, which in turn leads to hepatic inflammation. In addition, our findings indicate that female rats have a higher susceptibility to liver damage because of a reduced capacity for hepatoprotection.

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“…VYNCKE [ll] lists 28 compounds in fish trichloroacetic acid (TCA) extracts that react with 2-thiobarbituric acid. Malonaldehyde can form transverse bonds in proteins [ 121. Malonaldehyde-amine condensation products still give a colour reaction with 2-thiobarbituric acid but in reactions with amino acids, such as citrulline or arginine the recoveries of malonaldehyde by the TBA-colour test were 30 % and 6 %, respectively [6]. Results of the TBA test depend on temperature and duration of the reaction, which in turn depend on malonaldehyde precursors disintegration and release of malonaldehyde from binding with proteins [12, 131. In the process of TBA determination, malonaldehyde is formed from endoperoxides [5].…”

Section: Introductionmentioning

confidence: 99%

Some comments on the usefulness of 2‐thiobarbituric acid (TBA) test for the evaluation of rancidity in frozen fish

Kołakowska¹,

Deutry²

1983

Nahrung

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Rancidity of frozen fish (Baltic cod and herring, mackerel, horse mackerel and hake) was determined by means of TBA value, organoleptical scoring and peroxide value. The correlation between the rancid odour sensory assessment and TBA test results proved insignificant (r = 0.53). Model experiment was undertaken in which promine D with addition of papain was incubated at 50 degrees C over 44 h. It was shown that as accumulation of protein hydrolysis products proceeded the increase of TBA value has taken place without development of rancid odour and with peroxide content below sensibility of sulphocyanide method. With regard to fish, therefore, it seems that the TBA test applied by means of techniques in which the reaction with TBA proceeds in the presence of interfering substances should be treated as a "freshness test" rather than a strictly rancidity one.

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Amine‐malonaldehyde condensation products and their relative color contribution in the thiobarbituric acid test

Cited by 57 publications

References 16 publications

A specific, accurate, and sensitive measure of total plasma malondialdehyde by HPLC

A specific, accurate, and sensitive measure of total plasma malondialdehyde by HPLC

Lysosomal Leakage and Lack of Adaptation of Hepatoprotective Enzyme Contribute to Enhanced Susceptibility to Ethanol‐Induced Liver Injury in Female Rats

Some comments on the usefulness of 2‐thiobarbituric acid (TBA) test for the evaluation of rancidity in frozen fish

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