2013
DOI: 10.1194/jlr.d032698
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A specific, accurate, and sensitive measure of total plasma malondialdehyde by HPLC

Abstract: The process of lipid peroxidation results in a range of intermediates and end products including lipid hydroperoxides, aldyehydes, and malondialdehyde (MDA). These aldehydes and lipid hydroperoxides form DNA adducts and may result in extensive single-strand and doublestrand breaks ( 4 ). Various intermediates and end products generated during the lipid peroxidation cascade have been assayed, but the most-commonly employed approach continues to be the thiobarbituric acid (TBA) test ( 5 ).Although it is widely a… Show more

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Cited by 137 publications
(101 citation statements)
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References 28 publications
(31 reference statements)
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“…28 Thiobarbituric acid reactive substances assay was used to detect and quantify any MDAs formed. 33,34 It is a common method to measure oxidative change in lipids due to free radicals. 35 It was found that there was a continuous increase in the MDA formation in each hour (Figure 7).…”
Section: Mda Quantificationmentioning
confidence: 99%
“…28 Thiobarbituric acid reactive substances assay was used to detect and quantify any MDAs formed. 33,34 It is a common method to measure oxidative change in lipids due to free radicals. 35 It was found that there was a continuous increase in the MDA formation in each hour (Figure 7).…”
Section: Mda Quantificationmentioning
confidence: 99%
“…60 µl), from which plasma was obtained by centrifuging the sample at 7000 rpm for 5 min. We determined the levels of malondialdehyde (MDA) from the plasma and erythrocyte samples following standard procedures using derivatization with thiobarbituric acid followed by ultra-highperformance liquid chromatography (UHPLC) coupled to fluorescence detection (adapted from Agarwal and Chase, 2002;Moselhy et al, 2013). Over the course of the diquat treatment, we found that MDA levels in plasma gradually increased ( Fig.…”
Section: Materials and Methods Pilot Studymentioning
confidence: 97%
“…Малоновый диальдегид образуется при перекисном окислении ненасыщенных жир-ных кислот, таким образом являясь маркером ок-сидативного разрушения клеточных мембран [11]. Существует методика по хроматографическому определению малонового альдегида в плазме крови или сыворотке с помощью простой изократической жидкостной системы с флюоресцентной детекцией [12]. Флуорофор, получаемый в результате пробо-подготовки (осаждение белков и дериватизация), специфичен и детектируется в очень малых количе-ствах, при длине волны возбуждения 515 нм и длине волны эмиссии 553 нм.…”
Section: диагностика оксидативного стрессаunclassified